Results 151 to 160 of about 6,621,117 (310)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

a process-agent construction method for software process modeling in softpm

, 2006
Software development, unlike manufacturing industry, is highly dependent on the capabilities of individual software engineers and software development teams.
Xiao Junchao, Yuan Rong, Wang Qing, Li Mingshu, Zhang Lei, Nisar M. Wasif   +5 more
core   +1 more source

A tutorial on selecting, translating, and reporting variance priors in Bayesian multilevel models. [PDF]

Behav Res Methods
Liu L.
europepmc   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Other title: 2013 construction administration manual of procedures; Other title: Title from Office of Construction Administration home page: 2013 manual of procedures for construction inspection

, 2013
Accompanied by: Construction inspection forms booklet.This manual provides construction personnel with information to effectively administer the project and to perform accurate inspections and documentation of the construction work ...
Ohio. Department of Transportation. Office of Construction Administration.
core  

Vision and Development of a Design, Implementation, and Verification Automation (DIVA) Software Platform for DNA Construction. [PDF]

ACS Synth Biol
Plahar H, Lane SD, Stawski N, Simirenko L, Tapaswi M, Kuo R, Goyal G, Hillson NJ, Petzold CJ.   +8 more
europepmc   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Correction: Transcriptomic insights into the molecular regulatory mechanism of staminody in Chinese narcissus (<i>Narcissus tazetta</i> subsp. <i>chinensis</i>). [PDF]

Front Plant Sci
Zhao P, Yang Y, Zhang N, Fan K, Chang Y, Yan B, Tian H, Jiang Z, Ma Y, Hu T.   +9 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

PySimi: a unified framework for similarity measure evaluation in spectral clustering with applications to omics data. [PDF]

Front Genet
Shi T, Ye X, Zou Z, Xi W, Sakurai T.
europepmc   +1 more source