Results 131 to 140 of about 6,621,117 (310)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Integration of AR and deep learning–based image classification using CNN for construction project monitoring

Scientific Reports
With manual update, progress monitoring of a construction project is notably challenging due to its labor-intensive and time-consuming nature. The issue of manual update has been proposed to be alleviated by automatic update, but most existing AI-based ...
Su-Ling Fan, Bao-Yu Lin, Chun-Tin Wu, Hee Yong Youn, Faustyn Recha, Jakub Świerzawski, Marek Malucha   +6 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Final report : your building [PDF]

, 2007
The Your Building Project to build a website as the business and technical guide to\ud sustainable commercial buildings has now been completed. The site is available at\ud www.yourbuilding.org.

core  

Software Construction of Next-generation Locomotive Display Platform TPX20

机车电传动, 2010
This paper introduced the software of TPX20 display unit system which using linux operation system. It described the construction and kernel of the software system, discussed the device drivers and file system, as well as the optimization work focus on ...
谷丰, 刘群欣
doaj  

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Big data system software eco-system and platform construction

大数据, 2018
In view of the bottlenecks in common technology and engineering practice faced by big data system software and applications development,the key technological innovations of the National Engineering Laboratory for Big Data System Software(NEL-BDSS) were ...
Jianmin WANG, Chen WANG, Yingbo LIU, Lin LIU   +3 more
doaj  

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Typologies of offsite construction

, 2019
In the 21st century, where smart and modern technologies are developed at an expeditious rate, construction industry has survived over centuries, despite its slow rate of technology adaptations, poor productivity, lower sustainability and vastly reported
Ginigaddara, Buddhini (S34716), Perera, Srinath (R18622), Feng, Yingbin (R16766), Rahnamayiezekavat, Payam R. (R19191)   +3 more
core  

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source