Results 171 to 180 of about 2,082,662 (312)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

The development of a multi-level intervention to improve data quality of the national quality indicators in Swiss long-term care facilities using an Intervention Mapping approach. [PDF]

Front Health Serv
Osińska M, Braun D, Baumann S, Benkert B, Curreri N, Huang J, Simon M, Van Grootven B, Zúñiga F.   +8 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Artificial Intelligence in Dermatology: New Regulatory Aspects After the European AI Act. [PDF]

Skin Res Technol
Manfredini M, Romito CC, Viggiani S, Longo C.   +3 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Virtual Organizational Learnign in Open Source Software Development Projects [PDF]

We studied the existence of virtual organizational learning in open source software (OSS) development projects. Specifically, our research focused on learning effects of OSS projects and factors that affect the learning process. The number and percentage
Darrell Carpenter, Xiaogang Chen, Jan G. Clark, Yoris A. Au   +3 more
core  

Bridging the gap between public health, academia and policy. [PDF]

BMJ Glob Health
Christen P, Dawa J, Ahmed O, Akinyi F, Ameyo Masakhwe B, Bekalo DB, Biwot C, Bosire AB, Charles G, Gathoni V, Gaythorpe KAM, Geoffrey G, Gowa S, Hamumy FS, Kagiri H, Kamundia G, Kamunya S, Kariuki P, Khalayi L, Kombe I, Kwamboka P, Liétar P, Likalamu N, Lugonzo B, Mariita V, Masinde A, Mbithi M, McCabe R, Monari FN, Ondicho Motiri F, Muchiri AM, Mudamba A, Mugo PW, Mutono N, Muriithi M, Musasia R, Mutunga M, Mwangi W, Nalwa J, Ngeno KI, Nyaberi JM, Nzoka PM, Ojal J, Okech T, Okunga E, Omondi E, Osoro J, Osoro P, Patel A, Sayianka S, Shigoli T, Silali C, Thendu MM, van Elsland SL, Wanjiku KA, Muchemi P, Whittles LK, Winskill P, Ombajo LA, Thumbi SM, Watson OJ.   +60 more
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

User eXperience Perception Insights Dataset (UXPID): Synthetic User Feedback from Public Industrial Forums. [PDF]

Sci Data
Kulyabin M, Joosten J, Ulan Uulu C, Martins Pacheco NM, Ries F, Petridis F, Bosch J, Olsson HH.   +7 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source