Results 231 to 240 of about 4,581,706 (333)

Construction of hyperthermostable d‐allulose 3‐epimerase from Arthrobacter globiformis M30 using the sequence information from Arthrobacter psychrolactophilus

FEBS Open Bio, EarlyView.
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Kensaku Shimada, Kouhei Ohtani, Pushpa Kiran Gullapalli, Kazuhiko Ishikawa   +3 more
wiley   +1 more source

Applying a system dynamics approach for decision-making in software testing projects. [PDF]

PLoS One
Li W, Fang CC.
europepmc   +1 more source

Earthing effects on mitochondrial function: ATP production and ROS generation

FEBS Open Bio, EarlyView.
In contrast to sham and naive controls, grounded mitochondria not only exhibit significantly enhanced energy production but also demonstrate a remarkable reduction in membrane potential and oxidative stress. This suggests a profound improvement in mitochondrial health, presenting a promising avenue for therapeutic interventions.
Cecilia Giulivi, Richard Kotz
wiley   +1 more source

Heron, a Knowledge Graph editor for intuitive implementation of Python-based experimental pipelines. [PDF]

Elife
Dimitriadis G, Svahn E, MacAskill AF, Akrami A.   +3 more
europepmc   +1 more source

Biophysical characterization and ion transport with cell‐based and proteoliposome reconstitution assays of invertebrate K+‐Cl− cotransporters

FEBS Open Bio, EarlyView.
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo, Marina Verkhovskaya, Coralie Di Scala, Claudio Rivera, Tommi Kajander   +4 more
wiley   +1 more source

IDENTIFYING THE VALUES OF CUSTOMERS IN SOFTWARE DEVELOPMENT PROCESS [PDF]

Issues in Information Systems, 2012
Jeff Widman, Stella Hua, Steve Ross
doaj  

Live Cell Imaging to Monitor Axonal Pruning in <i>Drosophila</i> Motor Neurons. [PDF]

Bio Protoc
Long K, Xu W, Miao X, Wang S, Rui M.
europepmc   +1 more source

PROCESS MODELS AND DISTRIBUTION OF WORK IN OFFSHORING APPLICATION SOFTWARE DEVELOPMENT

, 2009
Karl Kurbel
openalex   +1 more source

Developing a Taxonomy for Software Process Context

Proceedings of the 13th International Conference on Software Technologies, 2018
James Buchan, Diana Kirk
openaire   +2 more sources

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source