SmallTalk: a novel small‐sized fusion tag for peptide expression and purification
FEBS Open Bio, EarlyView.The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq +3 more
wiley +1 more source
Somatic cell count in dairy goats II: udder health monitoring at goat and herd level. [PDF]
BMC Vet ResSmistad M +6 more
europepmc +1 more source
Autophagosome marker, LC3, is released extracellularly via several distinct pathways
FEBS Open Bio, EarlyView.This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito +3 more
wiley +1 more source
Association with Elevated Somatic Cell Counts and Characterization of Aerococcus viridans Isolates from Bovine Mastitis Milk in South Korea. [PDF]
Curr MicrobiolKang HJ +7 more
europepmc +1 more source
FEBS Open Bio, EarlyView.This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso +4 more
wiley +1 more source
The Role of Somatic Cell Synchronization in Nuclear Transfer and Induced Pluripotent Stem Cells for Wild Felids. [PDF]
Zoo BiolViana JVS, Pereira AF.
europepmc +1 more source
Pathogenic Neurofibromatosis type 1 gene variants in tumors of non‐NF1 patients and role of R1276
FEBS Open Bio, EarlyView.Somatic variants of the neurofibromatosis type 1 (NF1) gene occur across neoplasms without clinical manifestation of the disease NF1. We identified emerging somatic pathogenic NF1 variants and hotspots, for example, at the arginine finger 1276. Those missense variants provide fundamental information about neurofibromin's role in cancer.
Mareike Selig +7 more
wiley +1 more source
Machine Learning Approach for Early Lactation Mastitis Diagnosis Using Total and Differential Somatic Cell Counts. [PDF]
Animals (Basel)Zecconi A +5 more
europepmc +1 more source
Factors affecting somatic cell count in dairy goats: a review
, 2014 Rocío Jiménez-Granado +3 more
openalex +2 more sources