Results 91 to 100 of about 67,513 (334)

Microsurgical nuclear transfer by intraooplasmic karyoplast injection as an alternative embryo reconstruction method in somatic cloning of pigs and other mammal species; application value of the method and its technical advantages: a review

Czech Journal of Animal Science, 2005
The introduction of cell nuclei into enucleated recipient cells, beyond enucleation, is the most significant stage of somatic cloning procedure. Microsurgical transfer of somatic nuclei can be an alternative method of clonal nuclear-cytoplasmic hybrid ...
M. Samiec, M. Skrzyszowska
doaj   +1 more source

Interplay between RNA‐protein interactions and RNA structures in gene regulation

FEBS Open Bio, EarlyView.
Methodological advances in mapping transcriptome‐wide RNA‐protein interactions and RNA structures have started to uncover the potential of RNP conformations in gene regulation. Competing RNA–RNA, RNA‐protein and protein–protein interactions shape the compaction and function of RNPs throughout their lifetime and may provide novel therapeutic targets in ...
Jenni Rapakko, Mauro Scaravilli, Minna‐Liisa Änkö   +2 more
wiley   +1 more source

25th ANNIVERSARY OF CLONING BY SOMATIC-CELL NUCLEAR TRANSFER: Current applications of SCNT in advanced breeding and genome editing in livestock [PDF]

, 2021
Cesare Galli, Giovanna Lazzari
openalex   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Reproduction of Sheep through Nuclear Transfer of Somatic Cells: A Bibliometric Approach [PDF]

, 2023
José Roberto Vázquez-Avendaño, César Cortez‐Romero, Ángel Bravo-Vinaja, Demetrio Alonso Ambriz-García, Alfredo Trejo-Córdova, María del Carmen Navarro-Maldonado   +5 more
openalex   +1 more source

Molecular determinants of signal transduction in tropomyosin receptor kinases

FEBS Open Bio, EarlyView.
Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley   +1 more source

Long‐term culture of skin biopsies: maintenance of fibroblast production and competency of reprogramming

FEBS Open Bio, EarlyView.
Skin biopsies taken from a patient with an ultra‐rare disorder as well as controls were cultured for up to 473 days. The chunks of skin were serially transferred to a new culture plate when confluent with fibroblasts. Different generations of fibroblasts were analyzed for cell and molecular properties, proliferation, and competence for reprogramming to
Sudiksha Rathan‐Kumar, Michael A. Ripperger, Grant M. Westlake, Kevin C. Ess   +3 more
wiley   +1 more source

Telomere Elongation and Naive Pluripotent Stem Cells Achieved from Telomerase Haplo-Insufficient Cells by Somatic Cell Nuclear Transfer

Cell Reports, 2014
Summary: Haplo-insufficiency of telomerase genes in humans leads to telomere syndromes such as dyskeratosis congenital and idiopathic pulmonary fibrosis.
Li-Ying Sung, Wei-Fang Chang, Qian Zhang, Chia-Chia Liu, Jun-Yang Liou, Chia-Chun Chang, Huan Ou-Yang, Renpeng Guo, Haifeng Fu, Winston T.K. Cheng, Shih-Torng Ding, Chuan-Mu Chen, Maja Okuka, David L. Keefe, Y. Eugene Chen, Lin Liu, Jie Xu   +16 more
doaj   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Design, Screening, and Impact of sgRNAs Targeting Bovine Prolactin Gene Receptor on Embryonic Development Using Stably Transfected Cell Lines

Biology
This study designed three sgRNAs (sgRNA139, sgRNA128, and sgRNA109) targeting the prolactin gene receptor (PRLR) in fetal cattle, utilized Cas9 to cleave endogenous DNA, and screened stable cell lines for somatic cell nuclear transfer experiments to ...
Daqing Wang, Guifang Cao, Xin Li, Xin Cheng, Zhihui Guo, Lu Li, Hong Su, Kai Zhang, Yuanyuan Zhang, Min Zhang, Feifei Zhao, Yifan Zhao, Junxi Liang, Yiyi Liu, Yong Zhang   +14 more
doaj   +1 more source