Results 101 to 110 of about 67,513 (334)

Oct4-Transfection한 중간엽줄기세포 유래 핵이식 배반포의 Oct4 발현 분포 및 세포 자멸사의 변화에 관한 연구

Journal of Animal Reproduction and Biotechnology, 2016
There are various factors i.e. donor cell type, culture system as well as technical procedures which influence the pre-implantation embryonic development; however, may attempts have been made and still it is under investigation to improve the cloning ...
Won-Jae Lee, Jeong-Hyeon Lee, Gyu-Jin Rho , Sung-Lim Lee   +3 more
doaj   +1 more source

Somatic Cell Nuclear Transfer Followed by CRIPSR/Cas9 Microinjection Results in Highly Efficient Genome Editing in Cloned Pigs

International Journal of Molecular Sciences, 2016
The domestic pig is an ideal “dual purpose” animal model for agricultural and biomedical research. With the availability of genome editing tools such as clustered regularly interspaced short palindromic repeat (CRISPR) and associated nuclease Cas9 ...
Timothy P. Sheets, Chi-Hun Park, Ki‐Eun Park, A. Powell, D. Donovan, B. Telugu   +5 more
semanticscholar   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Cloned ferrets produced by somatic cell nuclear transfer

Developmental Biology, 2006
Somatic cell nuclear transfer (SCNT) offers great potential for developing better animal models of human disease. The domestic ferret (Mustela putorius furo) is an ideal animal model for influenza infections and potentially other human respiratory diseases such as cystic fibrosis, where mouse models have failed to reproduce the human disease phenotype.
Li, Ziyi, Sun, Xingshen, Chen, Juan, Liu, Xiaoming, Wisely, Samantha M., Zhou, Qi, Renard, Jean Paul, J. P., Leno, Gregory H., Engelhardt, John F.   +8 more
openaire   +3 more sources

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

High histone crotonylation modification in bovine fibroblasts promotes cell proliferation and the developmental efficiency of preimplantation nuclear transfer embryos

Scientific Reports
Lysine crotonylation (Kcr) is a recently discovered histone acylation modification that is closely associated with gene expression, cell proliferation, and the maintenance of stem cell pluripotency and indicates the transcriptional activity of genes and ...
Xiaoyu Zhao, Mengxin Du, Shanshan Wu, Zhiwen Du, Shuqin Liu, Lei Yang, Haoran Ma, Liguo Zhang, Lishuang Song, Chunling Bai, Guanghua Su, Guangpeng Li   +11 more
doaj   +1 more source

Histone Demethylase Expression Enhances Human Somatic Cell Nuclear Transfer Efficiency and Promotes Derivation of Pluripotent Stem Cells [PDF]

, 2015
Young Gie Chung, Shogo Matoba, Yuting Liu, Jin Hee Eum, Falong Lu, Wei Jiang, Jeoung Eun Lee, Vicken Sepilian, Kwang Yul, Dong Ryul Lee, Yi Zhang   +10 more
openalex   +1 more source

FGFR Like1 drives esophageal cancer progression via EMT, PI3K/Akt, and notch signalling: insights from clinical data and next‐generation sequencing analysis

FEBS Open Bio, EarlyView.
Clinical analysis reveals significant dysregulation of FGFRL1 in esophageal cancer (EC) patients. RNAi‐coupled next‐generation sequencing (NGS) and in vitro study reveal FGFRL1‐mediated EC progression via EMT, PI3K/Akt, and Notch pathways. Functional assays confirm its role in tumor growth, migration, and invasion.
Aprajita Srivastava, Anoop Saraya, Deepak Gunjan, Rinu Sharma   +3 more
wiley   +1 more source

Various nuclear reprogramming systems using egg and oocyte materials

The Journal of Reproduction and Development, 2019
Maternal factors stored in eggs and oocytes are necessary for reprogramming sperm for embryonic development. This reprogramming activity of maternal factors also works towards somatic cells, including terminally differentiated cells.
Kei MIYAMOTO
doaj   +1 more source

The effect of salubrinal on the endoplasmic reticulum stress pathway in heat‐stressed spermatogonial cells in vitro

FEBS Open Bio, EarlyView.
This study investigates the protective role of salubrinal against heat‐induced endoplasmic reticulum (ER) stress in mouse spermatogenic cells (GC1 and GC2). By modulating the ER stress pathway, salubrinal alleviates cellular stress and supports spermatogenic cell survival, suggesting its potential as a therapeutic candidate for heat‐related infertility.
Suna Karadeniz Saygili, Mustafa Oztatlici, Mahmut Kemal Ozbilgin   +2 more
wiley   +1 more source