Results 291 to 300 of about 63,552 (337)

Somatic cell nuclear transfer

Australian Journal of Experimental Agriculture, 2004
Nuclear transfer research became front-page news when the birth of Dolly, the cloned ewe, was reported by Ian Wilmut and Keith Campbell in 1997. Since Dolly’s birth, offspring from many other species have been produced using somatic cell nuclear transfer.
openaire   +3 more sources

Cloning of Monkeys by Somatic Cell Nuclear Transfer

2020
Somatic cell nuclear transfer (SCNT) is a promising method to establish genetically modified monkeys with identical genetic background as models in biomedical research. We have recently cloned monkeys by optimization of the SCNT protocols and inclusion of the epigenetic modulator.
Zhen Liu, Shiyu Sun, Zhaodi Liao, Qiang Sun   +3 more
openaire   +3 more sources

MC1568 Enhances Histone Acetylation During Oocyte Meiosis and Improves Development of Somatic Cell Nuclear Transfer Embryos in Pig.

Cellular Reprogramming, 2018
An increasing number of studies have revealed that histone deacetylase (HDAC) mediated histone deacetylation is important for mammalian oocyte development.
Huili Wang, Wei Cui, C. Meng, Jun Zhang, Yinxia Li, Y. Qian, Guangdong Xing, Dongmin Zhao, Shaoxian Cao   +8 more
semanticscholar   +1 more source

Human somatic cell nuclear transfer and cloning

Fertility and Sterility, 2012
This document presents arguments that conclude that it is unethical to use somatic cell nuclear transfer (SCNT) for infertility treatment due to concerns about safety; the unknown impact of SCNT on children, families, and society; and the availability of other ethically acceptable means of assisted reproduction.
Judith Daar, Leslie Francis, Sean Tipton, Paula Amato, Robert W. Rebar, Mark Gibson, John A. Robertson, Jean Benward, Bonnie Steinbock, Richard H. Reindollar, Mark Sauer, Steven Ralston, Monique Spillman, Robert Brzyski, Andrea Stein, Andrea Mechanick Braverman, Senait Fisseha, Dolores J. Lamb, Bruce Wilder   +18 more
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Cattle Cloning by Somatic Cell Nuclear Transfer

2023
Cloning by somatic cell Nuclear Transfer (SCNT) is a powerful technology capable of reprograming terminally differentiated cells to totipotency for generating whole animals or pluripotent stem cells for use in cell therapy, drug screening, and other biotechnological applications. However, the broad usage of SCNT remains limited due to its high cost and
Juliano Rodrigues, Sangalli, Rafael Vilar, Sampaio, Tiago Henrique Camara, De Bem, Lawrence Charles, Smith, Flávio Vieira, Meirelles   +4 more
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Establishment of a Somatic Cell Bank for Indian Buffalo Breeds and Assessing the Suitability of the Cryopreserved Cells for Somatic Cell Nuclear Transfer.

Cellular Reprogramming, 2018
Biobanks of cryopreserved gametes and embryos of domestic animals have been utilized to spread desired genotypes and to conserve the animal germplasm of endangered breeds. In principle, somatic cells can be used for the same purposes, and for reviving of
N. Selokar, Papori Sharma, A. Krishna, Deepak Kumar, Dharmendra Kumar, M. Saini, Arpna Sharma, K. Vijayalakshmy, P. Yadav   +8 more
semanticscholar   +1 more source

Animal Cloning by Somatic Cell Nuclear Transfer

2009
Animal cloning is becoming increasingly useful for its applications in biological inquiry and for its potential use in pharmaceutical, medical, and agricultural fields. Due to the complexity of the numerous steps required in reconstructing oocytes by nuclear transfer, detailed protocols are required to minimize the developmental damages inflicted ...
Jae Gyu Yoo, Lawrence C. Smith
openaire   +3 more sources

Procedures for Somatic Cell Nuclear Transfer in the Rat

2009
Somatic cell nuclear transfer (SCNT) is a powerful tool for the investigation of the mechanisms of nuclear remodeling. In addition, SCNT may offer the possibility of introducing targeted mutations by homologous recombination in species for which ES cell technology is not available.
Yacine Cherifi, Eryao Wang, Christelle Jacquet, Alexandre Fraichard, Qi Zhou, Jean Cozzi   +5 more
openaire   +3 more sources

Progress in Human Somatic-Cell Nuclear Transfer

New England Journal of Medicine, 2005
The efficiency of deriving blastocysts through the nuclear transfer of embryonic stem cells has been improved by a factor of more than 14.
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Method for somatic cell nuclear transfer in zebrafish

2016
This chapter presents a detailed methodology for somatic cell nuclear transfer-cloning of zebrafish. We aim to place the reader in a virtual lab experience to assist acquisition of the technical skills required for reproducing the published protocol.
Jose B. Cibelli, Jose B. Cibelli, Kannika Siripattarapravat   +2 more
openaire   +3 more sources