Detection and discovery of plant viruses in soybean by metagenomic sequencing
Background Viruses negatively impact soybean production by causing diseases that affect yield and seed quality. Newly emerging or re-emerging viruses can also threaten soybean production because current control measures may not be effective against them.
Manjula G. Elmore +10 more
doaj +1 more source
Transposons as Tools for Future Genome Engineering in Yeasts and Filamentous Fungi
Applications of transposon‐based tools for genome engineering in fungi include transposon‐mediated mutagenesis and targeted genomic integration. ABSTRACT Transposons are fundamental genetic elements that have profoundly shaped the architecture of eukaryotic genomes.
Bingyin Peng +4 more
wiley +1 more source
Genetic characterization of Pepino mosaic virus isolates from Belgian greenhouse tomatoes reveals genetic recombination [PDF]
Over a period of a few years, Pepino mosaic virus (PepMV) has become one of the most important viral diseases in tomato production worldwide. Infection by PepMV can cause a broad range of symptoms on tomato plants, often leading to significant financial ...
Wittemans, Lieve +18 more
core +1 more source
Plant phosphate (Pi) homeostasis relies on coordinated activities of the inositol polyphosphate kinases IPK1 and ITPK1, which balance localized InsP7 biosynthesis to control COP9 signalosome (CSN)‐dependent deneddylation of cullin 1 (CUL1). Perturbation of this equilibrium affects the stability of SPX4, a key negative regulator of phosphate starvation ...
Yashika Walia +12 more
wiley +1 more source
Detection of Soybean mosaic virus by Reverse Transcription Loop-mediated Isothermal Amplification
Soybean mosaic virus (SMV) is a prevalent pathogen that causes significant yield reduction in soybean production worldwide. SMV belongs to potyvirus and causes typical symptoms such as mild mosaic, mosaic and necrosis.
Yeong-Hoon Lee +9 more
doaj +1 more source
Natural Variation in GmSL20 Improves Seed Size and Quality in Soybean
ABSTRACT Seed size plays an important role in determining crop yield, yet the genetic mechanisms underlying the regulation of this agronomic trait remain incompletely understood in soybean. Here, we perform a genome‐wide association study mapping with seed length variations and identify a major quantitative trait locus (QTL) on chromosome 20, named ...
Yuechuan Luo +12 more
wiley +1 more source
Evidence for RNA recombination between distinct isolates of Pepino mosaic virus. [PDF]
Genetic recombination plays an important role in the evolution of virus genomes. In this study we analyzed publicly available genomic sequences of Pepino mosaic virus (PepMV) for recombination events using several bioinformatics tools.
Leunissen, Jack +9 more
core +1 more source
RNA‐Guided Engineering of the Chloroplast Genome Enabled by Plastid‐Expressed Guide RNAs
ABSTRACT Our goal is to develop RNA‐guided engineering of the chloroplast genome using the CRISPR/Cas9 system. We designed chloroplast minigenes to obtain properly sized single guide RNAs (sgRNAs) in tobacco chloroplasts. The sgRNA 5′ end is defined by transcription from an rRNA operon promoter, and its 3′ end by processing a downstream tRNA (trnG) or ...
Malihe Mirzaee +3 more
wiley +1 more source
Generation of Herbicide‐Resistant Alfalfa Germplasm via Prime Editing
ABSTRACT Alfalfa (Medicago sativa) is the most widely cultivated forage crop worldwide, owing to its high protein content, balanced amino acid profile, and abundance of vitamins and minerals. However, weed infestation severely threatens alfalfa yield and quality.
Shihao Li +5 more
wiley +1 more source
Coalescence of the sites of cowpea mosaic virus RNA replication into a cytopathic structure [PDF]
Cowpea mosaic virus (CPMV) replication induces an extensive proliferation of endoplasmic reticulum (ER) membranes, leading to the formation of small membranous vesicles where viral RNA replication takes place.
Carette, J.E. +3 more
core +1 more source

