Results 41 to 50 of about 5,188,114 (289)

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Longitudinal space charge debunching and compensation in high-frequency accelerators

Physical Review Special Topics. Accelerators and Beams, 1999
Space charge debunching is a major issue for future high-gradient, high-frequency accelerator techniques. Space charge will set limits on the maximum six-dimensional phase space density obtainable in optical or plasma based accelerators.
L. C. Steinhauer, W. D. Kimura
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Improved space charge transport model in bi-layer dielectrics—considering carrier dynamic equilibrium

High Voltage, 2020
The Maxwell–Wagner model and bipolar charge transport model both aim at describing the charge transport behaviours in dielectrics. The Maxwell–Wagner model performs well in calculating the polarisation current and electric field distribution, but it is ...
Hucheng Liang, Boxue Du, Jin Li, Hang Yao, Hang Yao, Zehua Wang   +5 more
doaj   +1 more source

Identification of a Shiga toxin A‐derived peptide internalized into Gb3 receptor‐bearing cells via interaction with the Shiga toxin B subunit

FEBS Letters, EarlyView.
The process of internalization of the Shiga toxin A subunit via formation of a complex with the Shiga toxin B subunit, which specifically binds to the Gb3 receptor. The peptide is designed to act as a carrier of drugs into cancer cells. Here, we explored the potential of peptides derived from the catalytic A subunit of Shiga toxin (STxA) to be drug ...
Giulia Opassi, João C. Encarnação, Valeria Napolitano, Grzegorz Dubin, Grzegorz Popowicz, Karl Andersson, U. Helena Danielson   +6 more
wiley   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Effect of DC Pre-stress on Space Charge and Electrical Tree in XLPE with Needle-Plate Electrodes

Zhongguo dianli, 2021
Space charge is one of the main factors that affect the electrical tree characteristics of HVDC cable insulation. Based on the bipolar charge transport model, this paper simulates the space charge distribution characteristics of the two-dimensional ...
Xiaobin XU, Aijing LIU, Yanjie LE, Hechen LIU, Zhanpeng GUO, Yunpeng LIU   +5 more
doaj   +1 more source

Discerning protein pools by selective staining with self‐labeling tags

FEBS Letters, EarlyView.
Cell surface proteins have an intra‐ and extracellular pool. Combining genetic fusion to self‐labeling tags that can be addressed with small molecule fluorophores allows separating these pools. We highlight recent developments and techniques for state‐of‐the‐art interrogation of cell surface proteins in the complex tissue setting.
Kati Fischermanns, Johannes Broichhagen
wiley   +1 more source

Dynamics of space charge and electroluminescence modelling in polyethylene

, 2012
Space charge has been recognized as an important factor contributing to the electrical failure of the cable insulation. Extensive efforts have been made to investigate space charge dynamics within polymeric insulations under electric stresses.
Zhao, Junwei
core   +1 more source

Space Charge Accumulation Characteristics in HVDC Cable under Temperature Gradient

Energies, 2020
One of the main issues that affect the development of high-voltage direct-current (HVDC) cable insulation is the accumulation of space charge. The load operation of an HVDC cable leads to the formation of a radially distributed temperature gradient (TG ...
Yifan Zhou, Wei Wang, Tailong Guo
doaj   +1 more source