Results 31 to 40 of about 67,105 (263)

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

open access: yesFEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa   +5 more
wiley   +1 more source

Space Charge Accumulation at Material Interfaces in HVDC Cable Insulation Part II—Simulations of Charge Transport

open access: yesEnergies, 2020
Extruded high voltage direct current (HVDC) cable systems contain interfaces with poorly understood microscopic properties, particularly surface roughness.
Espen Doedens   +3 more
doaj   +1 more source

Simulation study of the space charge limit in heavy-ion synchrotrons

open access: yesPhysical Review Accelerators and Beams, 2022
The SIS100 synchrotron as a part of the new Facility for Antiproton and Ion Research (FAIR) accelerator facility at GSI should be operated at the “space charge limit” for light- and heavy-ion beams.
Adrian Oeftiger   +5 more
doaj   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

open access: yesFEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Electron transfer between complexes III and IV in S. cerevisiae mitochondrial membranes

open access: yesFEBS Letters, EarlyView.
Mitochondrial oxidative phosphorylation in S. cerevisiae mitoplasts is limited by complex IV catalytic capacity, rather than two‐dimensional cytochrome c diffusion. At physiological cytochrome c : supercomplex ratios at salinity equivalent to that of 20 mm monovalent salt, activity is maximized, indicating that this low ionic strength accurately mimics
Ana Paula Lobez   +2 more
wiley   +1 more source

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

open access: yesFEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Space Charge Accumulation in Silicone Rubber Influenced by Poole-Frenkel Effect

open access: yesMATEC Web of Conferences, 2018
With the rapid increase of electrical energy consumption in metropolises, more and more power cables are utilized in power grid or urban power network to transmit and distribute electrical energy.
Kang Wenbin   +7 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

open access: yesFEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler   +2 more
wiley   +1 more source

Investigation on space charge and charge trap characteristics of gamma-irradiated epoxy micro–nano composites

open access: yesHigh Voltage, 2019
Epoxy nano–micro composite specimen prepared with micro silica and ion trapping nanoparticle, by shear mixing process, was exposed to gamma radiation and its performance for space charge and charge trap characteristics were analysed.
Myneni Sukesh Babu   +3 more
doaj   +1 more source

High-Precision Inertial Sensor Charge Management Based on Ultraviolet Discharge: A Comprehensive Review

open access: yesSensors, 2023
The charge accumulation caused by cosmic rays and solar energetic particles poses a significant challenge as a source of noise for inertial sensors used in space gravitational wave detection. To address this issue, the implementation of charge management
Tao Yu, Yuhua Wang, Yang Liu, Zhi Wang
doaj   +1 more source

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