Results 91 to 100 of about 6,162,711 (284)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Solution-processed conjugated polymer organic p-i-n light-emitting diodes with high built-in potential by solution- and solid-state doping

, 2009
10.1063/1.3257979Applied Physics Letters9521 ...
Chua, Lay-Lay, Mi Zhou, Peter K. H. Ho, Kumar, A.C., Lay-Lay Chua, Chandiran, Aravind Kumar, Png, Rui-Qi, Sankaran, Sivaramakrishnan, Zhou, M., Aravind C. Kumar, Png, R.-Q., Mi, Zhou, Sivaramakrishnan, S., Chen, Zhi-Li, Chen, Z.-L., K. H. Ho, Peter, Chua, L.-L., Sankaran Sivaramakrishnan, Zhi-Li Chen, Rui-Qi Png, Ho, P.K.H.   +20 more
core   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Editorial: Driving towards a more diverse space physics research community—perspectives, initiatives, strategies, and actions

Frontiers in Astronomy and Space Sciences, 2023
Michael W. Liemohn, Mcarthur Jones, Xochitl Blanco-Cano, John Coxon, Alexa J. Halford, Chigomezyo Ngwira, Chigomezyo Ngwira   +6 more
doaj   +1 more source

Unraveling the Mystery of Earth's Space Radiation Environment Loss Processes: Meeting Report

Earth and Space Science
On 10 June and 27 September 2024, two workshops were held at GFZ Potsdam under the umbrella of the Geo. X Research Network of Geosciences to discuss the unresolved question of the overestimation and lack of scattering of modeled ring current electrons ...
Bernhard Haas, Alexander Y. Drozdov, Jerry Goldstein, Raluca Ilie, Alwin Roy, Yangyang Shen, Katja Stoll, Ivan Vasko, Dedong Wang, Wei Wang   +9 more
doaj   +1 more source

Classical-physics applications for Finsler b space

, 2015
The classical propagation of certain Lorentz-violating fermions is known to be governed by geodesics of a four-dimensional pseudo-Finsler b space parametrized by a prescribed background covector field.
Ralf Lehnert, Joshua Foster
core   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Computational Physics for Space Flight Applications

, 2004
This paper presents viewgraphs on computational physics for space flight applications. The topics include: 1) Introduction to space radiation effects in microelectronics; 2) Using applied physics to help NASA meet mission objectives; 3) Example of ...
Reed, Robert A.
core  

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source