Results 101 to 110 of about 542,548 (263)

Research on the influence of passenger spatial perception on non-adaptive evacuation behavior in railway stations

Scientific Reports
This paper explores the mechanism of passengers’ spatial perceptions that influence their non-adaptive evacuation behavior in railway stations. Based on environment-behavior theory and S-O-R theory, it selects spatial perception, security atmosphere ...
Guochang Tang, Jiayue Wang
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Development of Spatial Memory: A Behavioral Study

NeuroSci
Although spatial memory has been widely studied in rodents, developmental studies involving humans are limited in number and sample size. We designed and studied the validity of two simple experimental setups for the evaluation of spatial memory and navigation development. The dataset of this study was composed of 496 schoolchildren, from 4 to 15 years
Konstantinos Kostakos, Alexandra Pliakopanou, Vasileios Meimaridis, Ourania-Natalia (Oriana) Galanou, Aikaterini Argyro Anagnostou, Dimitra Sertidou, Panagiotis Katis, Periklis Anastasiou, Konstantinos Katsoulidis, Yannis Lykogiorgos, Dimitrios Mytilinaios, Andreas P. Katsenos, Yannis V. Simos, Stefanos Bellos, Spyridon Konitsiotis, Dimitrios Peschos, Konstantinos I. Tsamis   +16 more
openaire   +3 more sources

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Identification of functional murine mitochondrial formyl peptides and their effects on myeloid‐derived suppressor cell generation

FEBS Open Bio, EarlyView.
We first identified functional murine mitochondrial N‐formyl peptides (MT‐FPs) and investigated their effects on the in vitro myeloid‐derived suppressor cell (MDSC) generation from bone marrow cells. We demonstrated that MT‐FPs acted directly on bone marrow cells to promote MDSC generation and modulated the polymorphonuclear (PMN)‐MDSC/monocyte (M ...
Miyako Ozawa, Saori Kagoshima, Akira Yamada   +2 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

The influence of garden spatial configuration on tourist behavior: A systematic review based on Space Syntax.

PLoS ONE
As composite spaces that integrate nature and culture, gardens are no longer regarded as merely static objects of visual appreciation in the context of urbanization, but have become essential venues for public cultural tourism and leisure.
Bin Li, Mohammad Mujaheed Hassan, Yan Han, Jasmine Leby Lau   +3 more
doaj   +1 more source

Spatial Optimization of Primary School Campuses from the Perspective of Children’s Emotional Behavior: A Deep Learning and Machine Learning Approach

Buildings
From the perspective of children’s emotional behavior, this study constructs a multidimensional indicator framework—“spatial elements-spatial typologies-spatial color-emotion and behavior.” Integrating behavior mapping, we employ deep- and machine ...
Ruiying Zhang, Binghuan Li, Qian Huang, Zhimou Peng, Yixun Xu, Li Tang, Zhiyue Ouyang, Xinyue Zhang, Lan Shang   +8 more
doaj   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source