Results 171 to 180 of about 4,357,372 (295)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Modeling Spatial Data with Heteroscedasticity Using PLVCSAR Model: A Bayesian Quantile Regression Approach. [PDF]

Entropy (Basel)
Chen R, Chen Z.
europepmc   +1 more source

sptotal: an R package for predicting totals and weighted sums from spatial data. [PDF]

J Open Source Softw, 2023
Higham M, Hoef JV, Frank B, Dumelle M.
europepmc   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

STHD: probabilistic cell typing of single spots in whole transcriptome spatial data with high definition. [PDF]

Genome Biol
Sun C, Zhang Y.
europepmc   +1 more source

The potential of small-scale spatial data in regional science. [PDF]

Jahrb Reg Wiss, 2022
Bergs R, Budde R.
europepmc   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Different Data for Different Goals: Exploring Trade-Offs and Synergies in the Use of Spatial Data Inputs to Optimize Conservation Action in Sagebrush Ecosystems. [PDF]

Ecol Evol
Shyvers JE, Tarbox BC, Monroe AP, Van Lanen NJ, Robb BS, Buchholtz EK, Duchardt CJ, Edmunds DR, O'Donnell MS, Van Schmidt ND, Heinrichs JA, Aldridge CL.   +11 more
europepmc   +1 more source

StarmapVis: An interactive and narrative visualisation tool for single-cell and spatial data. [PDF]

Comput Struct Biotechnol J, 2023
Ma S, Fang X, Yao Y, Li J, Morgan DC, Xia Y, Kwok CSM, Lo MCK, Siu DMD, Tsia KK, Yang A, Ho JWK.   +11 more
europepmc   +1 more source

Blood‐based proteomic profiling reveals context‐dependent changes in BCL2‐associated signaling during taxane therapy in breast cancer patients

FEBS Open Bio, EarlyView.
Chemotherapy side effects significantly impact cancer survivors' quality of life. Using protein levels in blood samples from breast cancer patients before and after 12 weeks of taxane treatment, we detected treatment‐dependent changes in calcium signaling and aging pathways associated with cancer recurrence.
Saira Munshani, Eiman Y. Ibrahim, Rozalyn L. Rodwin, Leah M. Ferrucci, Kim Blenman, Maryam Lustberg, Barbara E. Ehrlich   +6 more
wiley   +1 more source