Results 61 to 70 of about 326,434 (297)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Leaders And Followers In The Effectiveness Of Public Safety Services In European States – A Spatial Frontier Approach [PDF]

open access: yes, 2014
This research indicates that despite many differences among states’ public safety policies, improvement and regional development can be stimulated and achieved by implementing the “learning-from-the-best ...
Żółtaszek, Agata
core   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

The role of non-governmental actors in the spatial development of flood risks in Flanders (Belgium): towards a co-evolutionary approach in flood risk management [PDF]

open access: yes, 2016
Proceedings of the IV World Planning Schools Congress, July 3-8th, 2016 : Global crisis, planning and challenges to spatial justice in the north and in the southRecent challenges in flood risk management, call for the diversification of measures and the ...
Tempels, Barbara
core  

Spatial justice in participatory planning: an integrated framework and lessons from practice

open access: yesFrontiers in Sustainable Cities
Climate change is driving cities to transition toward more sustainable urban systems, often implementing these transitions through spatial interventions.
Juliana E. Gonçalves   +19 more
doaj   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

open access: yesFEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman   +4 more
wiley   +1 more source

Sexual Assault Study: Differences by Victim's Alcohol Use [PDF]

open access: yes, 2004
Poster originally presented to the Anchorage Police Department and the 2004 Alaska Summit on Violence Against Women.This issue of Anchorage Community Indicators Series 2, "Sexual Assault Study," describes the spatial patterning and geographical ...
Rosay, André B., Snodgrass, G. Matthew
core  

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

open access: yesFEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel   +8 more
wiley   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

open access: yesFEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Spatial justice in a world of violence [PDF]

open access: yes, 2017
Andreas Philippopoulos-Mihalopoulos opens the book with his latest contribution to his comprehensive project of re-theorising spatial justice with a piece titled ‘Spatial Justice in a World of Violence’. Through a close reading of the photographic series
Philippopoulos-Mihalopoulos, Andreas   +1 more
core  

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