Results 111 to 120 of about 68,356 (275)

Subcellular level spatial transcriptomics with PHOTON

Nature Communications
AbstractThe subcellular localization of RNA is closely linked to its function. Many RNA species are partitioned into organelles and other subcellular compartments for storage, processing, translation, or degradation. Thus, capturing the subcellular spatial distribution of RNA would directly contribute to the understanding of RNA functions and ...
Shreya Rajachandran, Qianlan Xu, Qiqi Cao, Xin Zhang, Fei Chen, Sarah M. Mangiameli, Haiqi Chen   +6 more
openaire   +4 more sources

Controlling 3D Contractility via Engineered Fibrous Hydrogel Composites

Advanced Functional Materials, EarlyView.
A tunable composite contractile assembly (CCA) is developed that can permit or resist contraction without changing cell‐adhesion and density, unlike collagen whose inherent coupling of these ECM cues limits its use as a platform in contractility studies.
Karen L. Xu, Yuqi Zhang, Alysse DeFoe, Georgios Kotsaris, Brendan Stoeckl, Matthew D. Davidson, Jason A. Burdick, Robert L. Mauck   +7 more
wiley   +1 more source

Computational modeling for deciphering tissue microenvironment heterogeneity from spatially resolved transcriptomics

Computational and Structural Biotechnology Journal
Spatial transcriptomics techniques, while measuring gene expression, retain spatial location information, aiding in situ studies of organismal tissue architecture and the progression of pathological processes.
Chuanchao Zhang, Lequn Wang, Qianqian Shi   +2 more
doaj   +1 more source

Synergistic All‐in‐One Electroceutical Platform Utilizing a Plasma–Photodynamic Hybrid Approach for Enhanced Wound Healing

Advanced Functional Materials, EarlyView.
A flexible hybrid patch integrating a robust cold atmospheric plasma (CAP) system with a high‐power, ultrathin bio‐OLED was developed to enable synergistic wound healing. This multimodal therapy, combining plasma and photodynamic treatment, enhanced angiogenesis, cell proliferation, and collagen deposition, demonstrating superior in vivo efficacy and ...
Jun‐Yeop Song, Hyun Cheol Bang, Young Hyun Son, Dae‐In Ha, Eun‐Ju Hong, Sun‐Woo Lee, Jeongsook Kim, Doyeong Kim, Sang‐Min Park, Kyu Pil Lee, Hyo‐Jung Kwon, Jeong Hyun Kwon   +11 more
wiley   +1 more source

Airway Spatial Transcriptomics in Smoking

AbstractBackgroundCigarette smoking has a significant impact on global health. Although cessation has positive health benefits, some molecular changes to intercellular communications may persist in the lung. In this study we created a framework to generate hypotheses by predicting altered cell-cell communication in smoker lungs using single-cell and ...
Jarrett D. Morrow, Zaid W. El-Husseini, Jeong H. Yun, Craig P. Hersh   +3 more
openaire   +2 more sources

SpotSweeper: spatially-aware quality control for spatial transcriptomics

Nature Methods
AbstractQuality control (QC) is a crucial step to ensure the reliability and accuracy of the data obtained from RNA sequencing experiments, including spatially-resolved transcriptomics (SRT). Existing QC approaches for SRT that have been adopted from single-nucleus RNA sequencing (snRNA-seq) methods are confounded by spatial biology and are ...
Totty, Michael, Hicks, Stephanie C., Guo, Boyi   +2 more
openaire   +2 more sources

SpaNorm: spatially-aware normalization for spatial transcriptomics data

Genome Biology
Normalization of spatial transcriptomics data is challenging due to spatial association between region-specific library size and biology. We develop SpaNorm, the first spatially-aware normalization method that concurrently models library size effects and the underlying biology, segregates these effects, and thereby removes library size effects without ...
Agus Salim, Dharmesh D. Bhuva, Carissa Chen, Chin Wee Tan, Pengyi Yang, Melissa J. Davis, Jean Y. H. Yang   +6 more
openaire   +3 more sources

Generation of an Induced Pluripotent Stem Cell‐Derived Alveolar Type II In Vitro Model to Study Influenza A Virus Infection and Drug Treatments

Advanced Healthcare Materials, EarlyView.
Patient‐specific induced pluripotent stem cells (iPSCs) can be differentiated into alveolar type II cells (iAT2s), expanded as 3D alveolospheres, and grown at physiologically relevant air–liquid interface (ALI). This study shows for the first time the infectability of iAT2s by the influenza A virus (IAV) and proves their responsiveness to the well ...
Lena Gauthier, Hristina Koceva, Yann Bachelot, Rosanne W. Koutstaal, Puck B. van Kasteren, Marc Thilo Figge, Christian Eggeling, Alexander Sandy Mosig   +7 more
wiley   +1 more source

Precise Control of Drug Release in Machine Learning‐Designed Antibody‐Eluting Implants for Postoperative Scarring Inhibition in Glaucoma

Advanced Healthcare Materials, EarlyView.
We developed a micro‐sized, biocompatible implant for postoperative sustained delivery of anti‐fibrotic antibodies in glaucoma surgery. Machine learning‐guided optimization of polymer composition, implant geometry, and porosity enabled precise control of drug release.
Mengqi Qin, Wenbing Jiang, Kai Xin Thong, Zeynep Ulker, Brihitejas Patel, Cynthia Yu‐Wai‐Man   +5 more
wiley   +1 more source

Flexible analysis of spatial transcriptomics data (FAST): a deconvolution approach

BMC Bioinformatics
Motivation Spatial transcriptomics is a state-of-art technique that allows researchers to study gene expression patterns in tissues over the spatial domain.
Meng Zhang, Joel Parker, Lingling An, Yiwen Liu, Xiaoxiao Sun   +4 more
doaj   +1 more source