Results 91 to 100 of about 45,113 (260)

An Ion‐Based Strategy Toward Synergistic Surface Functionalization Combining the Osteogenic Properties and NIR‐Mediated Antibacterial Activity of PEEK

Advanced Materials, EarlyView.
A multifunctional bioactive PEEK‐based implant (Mn@p/MBG‐SPEEK) is engineered to combat implant‐associated infections while promoting osteogenesis. The hybrid surface activates PI3K/Akt/mTOR and AP‐1 signaling pathways for osteogenic differentiation, while Mn coordination induces electronic coupling that narrows the HOMO‐LUMO energy gap and enhances ...
Zhiyan Xu, Ondřej Adam, Marek Doubrava, Ana M. Beltrán, Ivo Dlouhý, Xin Liu, Baiyan Sui, Aldo R. Boccaccini   +7 more
wiley   +1 more source

Recovering single-cell expression profiles from spatial transcriptomics with scResolve

Cell Reports: Methods
Summary: Many popular spatial transcriptomics techniques lack single-cell resolution. Instead, these methods measure the collective gene expression for each location from a mixture of cells, potentially containing multiple cell types.
Hao Chen, Young Je Lee, Jose A. Ovando-Ricardez, Lorena Rosas, Mauricio Rojas, Ana L. Mora, Ziv Bar-Joseph, Jose Lugo-Martinez   +7 more
doaj   +1 more source

Highly multiplexed spatial transcriptomics in bacteria

Science
Single-cell decisions made in complex environments underlie many bacterial phenomena. Image-based transcriptomics approaches offer an avenue to study such behaviors, yet these approaches have been hindered by the massive density of bacterial messenger RNA.
Ari Sarfatis, Yuanyou Wang, Nana Twumasi-Ankrah, Jeffrey R. Moffitt   +3 more
openaire   +3 more sources

Anisotropic Masked Mycobacterium Potentiates Amplified Antitumor Trained Immunity via Spleen Targeting and Myelopoiesis Conversion

Advanced Materials, EarlyView.
An engineered Trojan Mycobacterium, developed via a “peeling‐off and masking‐up” strategy, functions as a powerful splenic myelopoiesis converter. This spleen‐targeted nanomedicine induces trained immunity to reprogram immunosuppressive myeloid reservoirs into antitumoral effectors.
Jin‐Ho Choi, Sang Nam Lee, Jang Hun Heo, Soyoung Park, Sei Hyun Park, Yong Taik Lim   +5 more
wiley   +1 more source

Unsupervised spatially embedded deep representation of spatial transcriptomics

Genome Medicine
Optimal integration of transcriptomics data and associated spatial information is essential towards fully exploiting spatial transcriptomics to dissect tissue heterogeneity and map out inter-cellular communications.
Hang Xu, Huazhu Fu, Yahui Long, Kok Siong Ang, Raman Sethi, Kelvin Chong, Mengwei Li, Rom Uddamvathanak, Hong Kai Lee, Jingjing Ling, Ao Chen, Ling Shao, Longqi Liu, Jinmiao Chen   +13 more
doaj   +1 more source

Subcellular level spatial transcriptomics with PHOTON

Nature Communications
Abstract The subcellular localization of RNA is closely linked to its function. Many RNA species are partitioned into organelles and other subcellular compartments for storage, processing, translation, or degradation.
Shreya Rajachandran, Qianlan Xu, Qiqi Cao, Xin Zhang, Fei Chen, Sarah M. Mangiameli, Haiqi Chen   +6 more
openaire   +4 more sources

Engineering Pluripotent Stem Cells‐Derived Inner Ear Organoids With Enhanced Maturation and Reproducibility by Micro‐Topographical Cues

Advanced Materials, EarlyView.
Micro‐topographical cues applied through temporally controlled microscale confinement improve the reproducibility, spatial organization, and neurosensory‐associated features of pluripotent stem cell‐derived inner ear organoids. Integration with a vascularized organoid platform further enables controlled investigation of vascular‐epithelial interactions
Harshita Sharma, Jungeun Lim, Woochan Kim, Yeon Ju Kim, Dream Kim, Shinyull Lee, Chaeyeon Park, Stephen Rhee, Shruthy Kuttapan, Jungho Ha, Sang Wook Park, Kyunghoon Kim, Noo Li Jeon, Sunho Park, Yun‐Hoon Choung, Jangho Kim   +15 more
wiley   +1 more source

Flexible analysis of spatial transcriptomics data (FAST): a deconvolution approach

BMC Bioinformatics
Motivation Spatial transcriptomics is a state-of-art technique that allows researchers to study gene expression patterns in tissues over the spatial domain.
Meng Zhang, Joel Parker, Lingling An, Yiwen Liu, Xiaoxiao Sun   +4 more
doaj   +1 more source

SpotSweeper: spatially-aware quality control for spatial transcriptomics

Nature Methods
Abstract Quality control (QC) is a crucial step to ensure the reliability and accuracy of the data obtained from RNA sequencing experiments, including spatially-resolved transcriptomics (SRT). Existing QC approaches for SRT that have been adopted from single-nucleus RNA sequencing (snRNA-seq) methods are confounded by spatial biology ...
Totty, Michael, Hicks, Stephanie C., Guo, Boyi   +2 more
openaire   +4 more sources

GPR124 Alleviates Blood–Brain Barrier Disruption by Enhancing Microvascular Endothelial Function after Traumatic Brain Injury

Advanced Science, EarlyView.
Our study reveals the protective role of GPR124 in maintaining BBB integrity and promoting neurological recovery following TBI. It makes a significant contribution by uncovering a novel molecular interaction between GPR124 and FGFBP1 and linking this to activation of the Wnt/β‐catenin signaling pathway in vascular repair mechanisms.
Chen Wang, Lin Cai, Qiuyuan Gong, Yang Yang, Yuqing Liang, Xinyu Niu, Lai Wei, Ze Liu, Shengju Wu, Yinghui Men, Yaohui Tang, Jun Ding, Hengli Tian, Hao Chen   +13 more
wiley   +1 more source