Results 91 to 100 of about 3,101 (214)

Highly multiplexed spatial transcriptomics in bacteria

Science
Single-cell decisions made in complex environments underlie many bacterial phenomena. Image-based transcriptomics approaches offer an avenue to study such behaviors, yet these approaches have been hindered by the massive density of bacterial messenger RNA.
Ari Sarfatis, Yuanyou Wang, Nana Twumasi-Ankrah, Jeffrey R. Moffitt   +3 more
openaire   +3 more sources

A Dual‐Bioresponsive and Programmable Microneedle Matrix as a Bioinspired Coupler for Orchestrating Diabetic Bone Regeneration

Advanced Materials, EarlyView.
This project developed a smart bandage‐like patch (a microneedle array) for repairing diabetic bone damage. It intelligently senses signals from infection and inflammation, then releases its medicines in a specific, timed sequence: first an antibacterial agent, then an anti‐inflammatory agent, and finally growth factors.
Yu Wang, Rui Chen, Yu Chen, Haowen Lu, Yuchen Qian, Zhiheng Gao, Zheming Shen, Xiaofei Zheng, Huilin Yang, Jun Zou, Yusen Qiao   +10 more
wiley   +1 more source

Flexible analysis of spatial transcriptomics data (FAST): a deconvolution approach

BMC Bioinformatics
Motivation Spatial transcriptomics is a state-of-art technique that allows researchers to study gene expression patterns in tissues over the spatial domain.
Meng Zhang, Joel Parker, Lingling An, Yiwen Liu, Xiaoxiao Sun   +4 more
doaj   +1 more source

Subcellular level spatial transcriptomics with PHOTON

Nature Communications
Abstract The subcellular localization of RNA is closely linked to its function. Many RNA species are partitioned into organelles and other subcellular compartments for storage, processing, translation, or degradation.
Shreya Rajachandran, Qianlan Xu, Qiqi Cao, Xin Zhang, Fei Chen, Sarah M. Mangiameli, Haiqi Chen   +6 more
openaire   +4 more sources

Cell Adhesion by Design: Engineering Tissue Culture Scaffolds With Adhesion Cues

Advanced Materials Interfaces, EarlyView.
ABSTRACT In scaffold‐based tissue engineering, the matrix should provide adequate adhesion cues for cell attachment, spreading, and function. Given the multitude of adhesion receptors and the diversity of scaffolds, there are many approaches to render scaffolds adhesive, even though they are not all equivalent.
Dalia Dranseike, Dhananjay V. Deshmukh, Mark W. Tibbitt, Céline Labouesse   +3 more
wiley   +1 more source

Protocol for acquiring high-quality fresh mouse lung spatial transcriptomics data

STAR Protocols
Summary: Spatial transcriptomics analysis allows the examination of the biological characteristics and spatial distribution of individual lung cells at a single-cell resolution.
Yujia Jiang, Yixin Li, Mengnan Cheng, Jiangshan Xu, Xiaoyu Wei, Chuanyu Liu   +5 more
doaj   +1 more source

SpotSweeper: spatially-aware quality control for spatial transcriptomics

Nature Methods
Abstract Quality control (QC) is a crucial step to ensure the reliability and accuracy of the data obtained from RNA sequencing experiments, including spatially-resolved transcriptomics (SRT). Existing QC approaches for SRT that have been adopted from single-nucleus RNA sequencing (snRNA-seq) methods are confounded by spatial biology ...
Totty, Michael, Hicks, Stephanie C., Guo, Boyi   +2 more
openaire   +4 more sources

Chromosomal Instability Drives Glioblastoma Heterogeneity and Therapeutic Opportunities

Advanced Science, EarlyView.
ABSTRACT Glioblastoma, the most aggressive and lethal form of brain cancer, is defined by profound genomic instability, with Chromosomal Instability (CIN) playing a central role in driving tumor progression, therapy resistance, and poor prognosis. CIN is characterized by numerical and structural alterations, is driven by mechanisms such as mitotic ...
Amarnath Pal, Milan Patra, Rianita Mondal, Shubhasis Haldar   +3 more
wiley   +1 more source

Recovering single-cell expression profiles from spatial transcriptomics with scResolve

Cell Reports: Methods
Summary: Many popular spatial transcriptomics techniques lack single-cell resolution. Instead, these methods measure the collective gene expression for each location from a mixture of cells, potentially containing multiple cell types.
Hao Chen, Young Je Lee, Jose A. Ovando-Ricardez, Lorena Rosas, Mauricio Rojas, Ana L. Mora, Ziv Bar-Joseph, Jose Lugo-Martinez   +7 more
doaj   +1 more source

GPR124 Alleviates Blood–Brain Barrier Disruption by Enhancing Microvascular Endothelial Function after Traumatic Brain Injury

Advanced Science, EarlyView.
Our study reveals the protective role of GPR124 in maintaining BBB integrity and promoting neurological recovery following TBI. It makes a significant contribution by uncovering a novel molecular interaction between GPR124 and FGFBP1 and linking this to activation of the Wnt/β‐catenin signaling pathway in vascular repair mechanisms.
Chen Wang, Lin Cai, Qiuyuan Gong, Yang Yang, Yuqing Liang, Xinyu Niu, Lai Wei, Ze Liu, Shengju Wu, Yinghui Men, Yaohui Tang, Jun Ding, Hengli Tian, Hao Chen   +13 more
wiley   +1 more source