Results 71 to 80 of about 71,340 (293)

Single-cell transcriptomics : a high-resolution avenue for plant functional genomics [PDF]

, 2019
Plant function is the result of the concerted action of single cells in different tissues. Advances in RNA-seq technologies and tissue processing allow us now to capture transcriptional changes at single-cell resolution.
Finch, Jessica A., Lucas, Emma S., Ott, Sascha, Rich-Griffin, Charlotte, Schäfer, Patrick, Stechemesser, Annika   +5 more
core   +1 more source

Hijacking emergency granulopoiesis: Neutrophil ontogeny and reprogramming in cancer

Molecular Oncology, EarlyView.
Neutrophils are highly plastic innate immune cells; their functions in cancer extend beyond the tumour microenvironment. This Review summarises current understanding of neutrophil maturation and heterogeneity and highlights tumour‐induced granulopoiesis as a systemic programme that expands immature, immunosuppressive neutrophils via tumour‐derived ...
Gabriela Marinescu, Yi Feng
wiley   +1 more source

Integrating multiple spatial transcriptomics data using community-enhanced graph contrastive learning.

PLoS Computational Biology
Due to the rapid development of spatial sequencing technologies, large amounts of spatial transcriptomic datasets have been generated across various technological platforms or different biological conditions (e.g., control vs. treatment).
Wenqian Tu, Lihua Zhang
doaj   +1 more source

Spatial transcriptomics in breast cancer: providing insight into tumor heterogeneity and promoting individualized therapy

Frontiers in Immunology
A comprehensive understanding of tumor heterogeneity, tumor microenvironment and the mechanisms of drug resistance is fundamental to advancing breast cancer research. While single-cell RNA sequencing has resolved the issue of “temporal dynamic expression”
Junsha An, Junsha An, Junsha An, Yajie Lu, Yuxi Chen, Yuling Chen, Zhaokai Zhou, Jianping Chen, Cheng Peng, Ruizhen Huang, Fu Peng, Fu Peng   +11 more
doaj   +1 more source

SiGra: single-cell spatial elucidation through an image-augmented graph transformer

Nature Communications, 2023
Recent advances in high-throughput molecular imaging have pushed spatial transcriptomics technologies to subcellular resolution, which surpasses the limitations of both single-cell RNA-seq and array-based spatial profiling.
Ziyang Tang, Zuotian Li, Tieying Hou, Tonglin Zhang, Baijian Yang, Jing Su, Qianqian Song   +6 more
doaj   +1 more source

Metastasis on pause: How dormant tumor cells stay hidden within the tumor microenvironment and evade immune surveillance

Molecular Oncology, EarlyView.
Dormant cancer cells can hide in distant organs for years, evading treatment and the immune system. This review highlights how signals from the surrounding tissue and immune environment keep these cells inactive or trigger their reawakening. Understanding these mechanisms may help develop therapies to eliminate or control dormant cells and prevent ...
Kanishka Tiwary, Jose Javier Bravo‐Cordero   +1 more
wiley   +1 more source

Molecular landscape of the mouse adrenal gland and adjacent adipose tissue by spatial transcriptomics

Folia Histochemica et Cytobiologica
INTRODUCTION: . The adrenal glands are central regulators of endocrine homeostasis and stress adaptation. Despite decades of research, the molecular mechanisms governing adrenal zonation and cortical renewal remain incompletely understood.
Malgorzata Blatkiewicz, Szymon Hryhorowicz, Marta Szyszka, Joanna Suszynska-Zajczyk, Andrzej Plawski, Adam Plewinski, Andrea Porzionato, Ludwik K. Malendowicz, Marcin Rucinski   +8 more
doaj   +1 more source

Systems analysis of host-parasite interactions. [PDF]

, 2015
Parasitic diseases caused by protozoan pathogens lead to hundreds of thousands of deaths per year in addition to substantial suffering and socioeconomic decline for millions of people worldwide.
Jamshidi, Neema, Lewis, Nathan E, Swann, Justine, Winzeler, Elizabeth A   +3 more
core   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source