Results 121 to 130 of about 68,356 (275)

Spatially informed graph transformers for spatially resolved transcriptomics

Communications Biology
Spatially resolved transcriptomics (SRT) has emerged as a powerful technique for mapping gene expression landscapes within spatial contexts. However, significant challenges persist in effectively integrating gene expression with spatial information to elucidate the heterogeneity of biological tissues.
Xinyu Bao, Xiaosheng Bai, Xiaoping Liu, Qianqian Shi, Chuanchao Zhang   +4 more
openaire   +3 more sources

Preliminary Study of a Ninj1‐Loaded Bimodal Ultrasound/NIR Fluorescence Targeted Molecular Probe for Diagnosing Early‐Stage Inflammation in Coronary Microvascular Dysfunction

Advanced Healthcare Materials, EarlyView.
Coronary microvascular dysfunction (CMD) targeting remains a challenge for precise diagnosis. This work presents a dual‐modal nanoprobe (T‐IR780‐NBs) that combines ultrasound contrast with near‐infrared fluorescence. This technology utilizes proteomics‐derived antibodies that specifically localize to inflamed and injured cardiac tissue, enabling ...
Xiaohui Xu, Lina Guan, Baihetiya Tayier, Chen Yuan, Shangke Chen, Qi Shi, Yuming Mu   +6 more
wiley   +1 more source

Designing spatial transcriptomic experiments

Nature Methods, 2023
Dario Righelli, Andrea Sottosanti, Davide Risso   +2 more
openaire   +5 more sources

Infusible Extracellular Matrix Biomaterial Enhances Cell‐Specific Pro‐Repair Responses Following Acute Myocardial Infarction

Advanced Healthcare Materials, EarlyView.
We measure the cell‐specific responses of administering infusible ECM (iECM) in acute myocardial infarction (MI) across multiple timepoints. Using single‐nucleus RNA sequencing and spatial transcriptomics, we measure macrophage activation, fibroblast remodeling, increased vascular development, lymphangiogenesis, cardioprotection, and neurogenesis ...
Joshua M. Mesfin, Alexander Chen, Quincy P. Lyons, Michael B. Nguyen, Maria L. Karkanitsa, Justin Yu, Van K. Ninh, Emily Gardner, Elyse G. Wong, Sriya N. Paleti, Julian Cheng, Benjamin D. Bridgelal, Kate E. Reimold, Selena Cao, Connor Uhre, Colin G. Luo, Zhenxing Fu, Kevin R. King, Karen L. Christman   +18 more
wiley   +1 more source

Protocol for acquiring high-quality fresh mouse lung spatial transcriptomics data

STAR Protocols
Summary: Spatial transcriptomics analysis allows the examination of the biological characteristics and spatial distribution of individual lung cells at a single-cell resolution.
Yujia Jiang, Yixin Li, Mengnan Cheng, Jiangshan Xu, Xiaoyu Wei, Chuanyu Liu   +5 more
doaj   +1 more source

Rosetta Brains: A Strategy for Molecularly-Annotated Connectomics [PDF]

, 2014
We propose a neural connectomics strategy called Fluorescent In-Situ Sequencing of Barcoded Individual Neuronal Connections (FISSEQ-BOINC), leveraging fluorescent in situ nucleic acid sequencing in fixed tissue (FISSEQ).
Boyden, Edward S, Church, George M, Daugharthy, Evan R, Kalhor, Reza, Kebschull, Justus M, Kording, Konrad P, Lee, Je Hyuk, Marblestone, Adam H, Mishchenko, Yuriy, Peikon, Ian D, Shipman, Seth L, Zador, Anthony M   +11 more
core  

SiO2‐CaOCME/Poly(Tetrahydrofuran)/Poly(Caprolactone) 3D‐Printed Scaffolds Drive Human‐Bone Marrow Stromal Cell Osteogenic Differentiation

Advanced Healthcare Materials, EarlyView.
3D printed hybrid scaffolds combining bioactive silica–calcium chemistry with elastic polymers guide human bone stem cells to form bone. The scaffolds support cell survival, organization, and invasion while releasing osteogenic ions. Together, architecture and composition drive bone‐specific gene expression, extracellular matrix organization, and ...
David R. Sory, Agathe C.M. Heyraud, Julian R. Jones, Sara M. Rankin   +3 more
wiley   +1 more source

Recovering single-cell expression profiles from spatial transcriptomics with scResolve

Cell Reports: Methods
Summary: Many popular spatial transcriptomics techniques lack single-cell resolution. Instead, these methods measure the collective gene expression for each location from a mixture of cells, potentially containing multiple cell types.
Hao Chen, Young Je Lee, Jose A. Ovando-Ricardez, Lorena Rosas, Mauricio Rojas, Ana L. Mora, Ziv Bar-Joseph, Jose Lugo-Martinez   +7 more
doaj   +1 more source

Biomimetic Copper‐Doped Nano‐Aluminum Adjuvant Potentiates Therapy in Chemoresistant Acute Myeloid Leukemia

Advanced Healthcare Materials, EarlyView.
We developed a novel copper‐doped aluminum nano‐adjuvant (CuNA) to overcome cytarabine resistance in acute myeloid leukemia (AML). CuNA effectively sensitizes drug‐resistant AML cells to cytarabine by inducing mitochondrial dysfunction and inhibiting HMGCR/GPX4 to amplify ferroptosis.
Chao He, Lingxiao Zhang, Yue Xiong, Yu Wang, Yuqing Zeng, Dandan Su, Ran Wang, Ying Li, Hongbo Chen, Fang Cheng, Zhi Ping Xu   +10 more
wiley   +1 more source

stGRL: spatial domain identification, denoising, and imputation algorithm for spatial transcriptome data based on multi-task graph contrastive representation learning

BMC Biology
Background Spatial transcriptomics now enables sequencing while preserving the spatial location of cells. This significantly enhances researchers' understanding of cellular and tissue functions in their spatial context.
Xin Lu, Murong Zhou, Bo Gao, Fang Wang, Shuilin Jin, Qiaoming Liu, Guohua Wang   +6 more
doaj   +1 more source