Results 91 to 100 of about 9,088 (236)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Médicos recém-formados: sólida formação geral ou sólida formação especializada?

Revista Brasileira de Educação Médica
OBJETIVO: Investigar os efeitos da especialização precoce - dedicação seletiva a uma especialidade médica no período de graduação - sobre a formação geral dos médicos recém-formados.
Rafael Casali Ribeiro, Cristina Helena Ferreira Fonseca-Guedes, Maria do Patrocínio Tenório Nunes   +2 more
doaj   +1 more source

Developing physical education as a 'connective specialism': is sport education the answer?

, 2002
This paper addresses the challenge identified by Penney and Chandler [(2000) Physical education: what future(s)? Sport, Education and Society, 5(1), pp. 71-87] for physical education to develop as a 'connective specialism' [Young (1998) The Curriculum of
Penney, Dawn, Kinchin, Gary, Clarke, Gill, Gary Kinchin, Gill Clarke, Dawn Penney   +5 more
core   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

Growing a primary science specialism: assembling people, places, materials and ideas

, 2015
This paper derives from the authors’ experiences of the development of a successful science specialism, implemented in a large primary school in regional Victoria, Australia, since 2012.
L Sharp (13316577), K McCarthy (13679029), N Frankel (13679026), Julianne Lynch (13076376)   +3 more
core  

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Managing febrile neutropenia in adult cancer patients: an integrative review of the literature

Revista Brasileira de Enfermagem
Objective: To analyze the interventions performed by health professionals with a view to managing chemotherapy-induced febrile neutropenia. Method: Integrative literature review, the sample of 12 primary articles was selected from the following ...
Juliana Nunes Ferreira, Lury Renata Barbosa Ribeiro Correia, Renata Moreira de Oliveira, Silvia Naomi Watanabe, João Francisco Possari, Antônio Fernandes Costa Lima   +5 more
doaj   +1 more source