Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Gas Chromatography-Mass Spectrometry (GC-MS) and evaluation of antioxidant and antimicrobial activities of essential oil of Campomanesia adamantium (Cambess.) O. Berg (Guavira) [PDF]
, 2009 Isabel Duarte Coutinho +6 more
openalex +1 more source
Mass spectrometry-based metabolomics in microbiome investigations
Nature Reviews Microbiology, 2021 A. Bauermeister +4 more
semanticscholar +1 more source
Thrombolytic proteins profiling: High‐throughput activity, selectivity, and resistance assays
FEBS Open Bio, EarlyView.We present optimized biochemical protocols for evaluating thrombolytic proteins, enabling rapid and robust screening of enzymatic activity, inhibition resistance, and fibrin affinity, stimulation, and selectivity. The outcome translates to key clinical indicators such as biological half‐life and bleeding risk. These assays streamline the development of
Martin Toul +3 more
wiley +1 more source
In Silico Optimization of Mass Spectrometry Fragmentation Strategies in Metabolomics [PDF]
, 2019 Joe Wandy +5 more
openalex +1 more source
Molecular determinants of signal transduction in tropomyosin receptor kinases
FEBS Open Bio, EarlyView.Tropomyosin receptor kinases control critical neuronal functions, but how do the same receptors produce diverse cellular responses? This review explores the structural mechanisms behind Trk signaling diversity, focusing on allosteric modulation and ligand bias.
Giray Enkavi
wiley +1 more source
Edible oils and fats authentication by Fourier transform Raman spectrometry. Analytical methods for the authentification of agro-food products. Gembloux (Belgium). 20 Oct 1999. [PDF]
Biotechnologie, Agronomie, Société et Environnement, 2000 The European project FAIR-CT96-5053 concerned the application of the Fourier transform Raman and infrared spectroscopy in food chemistry and quality control.
Baeten, V., Aparicio R.
doaj
FEBS Open Bio, EarlyView.The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan +6 more
wiley +1 more source
Spectroscopic methods for authentification. An overview. Analytical methods for the authentification of agro-food products. Gembloux (Belgium). 20 Oct 1999. [PDF]
Biotechnologie, Agronomie, Société et Environnement, 2000 An analysis by the probability shows the criterion that an analysis method has to satisfy to allow the authentication of a product. There are two distinct ways of achieving this criterion.
Belton P.
doaj