Results 101 to 110 of about 1,232,276 (317)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Tandem Native Mass-Spectrometry on Antibody-Drug Conjugates and Submillion da Antibody-Antigen Protein Assemblies on an Orbitrap EMR Equipped with a High-Mass Quadrupole Mass Selector [PDF]

, 2015
Native mass spectrometry is emerging as a powerful tool for the characterization of intact antibodies and antibody-based therapeutics. Here, we demonstrate new possibilities provided by the implementation of a high mass quadrupole mass selector on the ...
Wang, Guanbo, Alexander Makarov (1319898), Heck, Albert J R, Albert J. R. Heck (9680), Andrey Dyachenko (1405822), Ewald T. J. van den Bremer (1583836), Sub Biomol.Mass Spectrometry & Proteom., Biomolecular Mass Spectrometry and Proteomics, Guanbo Wang (1329408), Sub Biomol.Mass Spect. and Proteomics, Mike Belov (1583833), Dyachenko, Andrey, Makarov, Alexander, Parren, Paul W H I, Paul W. H. I. Parren (215516), Belov, Mike, De Jong, Rob N., Van Den Bremer, Ewald T J, Rob N. de Jong (843705)   +18 more
core   +1 more source

Medication adherence during laboratory workup for primary aldosteronism: pilot study

Patient Preference and Adherence, 2018
Friederike A Sandbaumhüter,1,2 Manuel Haschke,1,2 Bruno Vogt,3 Jürgen M Bohlender1,2 1Institute of Pharmacology, University of Bern, Bern, Switzerland, 2Department of Clinical Pharmacology, Inselspital, Bern University Hospital, University of ...
Sandbaumhüter FA, Haschke M, Vogt B, Bohlender JM   +3 more
doaj  

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Parsimonious Charge Deconvolution for Native Mass Spectrometry

, 2018
Charge deconvolution infers the mass from mass over charge (m/z) measurements in electrospray ionization mass spectra. When applied over a wide input m/z or broad target mass range, charge-deconvolution algorithms can produce artifacts, such as false ...
Christopher Becker (1298982), Carlson, Eric, Doron Kletter, Yong J. Kil, Tomislav Caval, Franc, Vojtech, K. Ilker Sen (2311798), Dominique Hagemans, K. Ilker Sen, Becker, Christopher, Nicolas Galy, Marshall Bern (802724), Dominique Hagemans (4840668), Kil, Yong J, Eric Carlson, Eric Carlson (3928799), Nicolas Galy (4840665), Vojtech Franc, Caval, Tomislav, Christopher Becker, Hagemans, Dominique, Sen, K Ilker, Heck, Albert J R, Albert J. R. Heck (9680), Kletter, Doron, Doron Kletter (802722), Vojtech Franc (3817549), Galy, Nicolas, Yong J. Kil (2058790), Marshall Bern, Sub Biomol.Mass Spectrometry & Proteom., Biomolecular Mass Spectrometry and Proteomics, Wilfred Tang (4840671), Bern, Marshall, Tomislav Caval (3622946), Wilfred Tang, Albert J. R. Heck, Tang, Wilfred, Afd Biomol.Mass Spect. and Proteomics   +38 more
core   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Influence of Water on Aging Phenomena of Calendric Stored and Cycled Li-Ion Batteries

Nanoenergy Advances
We examine the impact of water (160 ± 41 ppm of reference) on the anode, cathode, separator and electrolyte in two aging scenarios: calendric aging (60 °C, 80 days, charged state), resulting in a triggered current interrupt device (CID), and cycling 1680
Gudrun Wilhelm, Ute Golla-Schindler, Katharina Wöhrl, Christian Geisbauer, Graham Cooke, Timo Bernthaler, Hans-Georg Schweiger, Gerhard Schneider   +7 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

FEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu, Lanlan Li, Wenjuan Jia, Hanzhong Feng, Xiaoquan Yu, Youli Zhao, Fengzhi Yuan, Hui Ding, Junqiang Tian, Yong‐Xing He, Zhiping Wang   +10 more
wiley   +1 more source

Investigating the interactions and conformations of metalloproteins by means of mass spectrometry [PDF]

The use of mass spectrometry for the study of proteins and their non-covalent interactions has expanded rapidly since the introduction of ionisation techniques capable of transferring intact complexes into the gas phase.
Kondrat, Frances D. L.
core