Results 51 to 60 of about 1,589,502 (297)

The IQ‐compete assay for measuring mitochondrial protein import efficiencies in living yeast cells

FEBS Letters, EarlyView.
The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Yasmin Hoffman, Annika Egeler, Saskia Rödl, Johannes M. Herrmann   +3 more
wiley   +1 more source

Spectral Characterization of Avocado Persea Americana Mill. Cv. Hass Using Spectrometry and Imagery from the Visible to Near-Infrared Range

TecnoLógicas, 2023
Remote sensing technologies, such as spectral imaging, have great potential for crop monitoring. Spectral systems measure the energy reflected and emitted by a surface, typically between the visible and near-infrared regions of the electromagnetic ...
Maria C. Torres-Madronero, Tatiana Rondón, Ricardo Franco, Maria Casamitjana, Johana Trochez González   +4 more
doaj   +1 more source

The Caenorhabditis elegans DPF‐3 and human DPP4 have tripeptidyl peptidase activity

FEBS Letters, EarlyView.
The dipeptidyl peptidase IV (DPPIV) family comprises serine proteases classically defined by their ability to remove dipeptides from the N‐termini of substrates, a feature that gave the family its name. Here, we report the discovery of a previously unrecognized tripeptidyl peptidase activity in DPPIV family members from two different species.
Aditya Trivedi, Rajani Kanth Gudipati
wiley   +1 more source

MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis

Frontiers in Microbiology, 2015
Currently microorganisms are best identified using 16S rRNA and 18S rRNA gene sequencing. However, in recent years matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a potential tool for microbial ...
N. Singhal, Manish Kumar, P. Kanaujia, J. S. Virdi   +3 more
semanticscholar   +1 more source

Peptide‐based ligand antagonists block a Vibrio cholerae adhesin

FEBS Letters, EarlyView.
The structure of a peptide‐binding domain of the Vibrio cholerae adhesin FrhA was solved by X‐ray crystallography, revealing how the inhibitory peptide AGYTD binds tightly at its Ca2+‐coordinated pocket. Structure‐guided design incorporating D‐amino acids enhanced binding affinity, providing a foundation for developing anti‐adhesion therapeutics ...
Mingyu Wang, Grace Du, Charity Yongo‐Luwawa, Angelina Lu, Brett Kinrade, Kim Munro, Karl E. Klose, William D. Lubell, Peter Davies, Shuaiqi Guo   +9 more
wiley   +1 more source

Iron Forms Fe(II) and Fe(III) Determination in Pre-Roman Iron Age Archaeological Pottery as a New Tool in Archaeometry

Molecules, 2021
This article presents studies on iron speciation in the pottery obtained from archaeological sites. The determination of iron forms Fe(II) and Fe(III) has been provided by a very simple test that is available for routine analysis involving the technique ...
Lidia Kozak, Andrzej Michałowski, Jedrzej Proch, Michal Krueger, Octavian Munteanu, Przemyslaw Niedzielski   +5 more
doaj   +1 more source

Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer

FEBS Letters, EarlyView.
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani, Noemi E. Nwosu, Viktoria Gocke, Müge Kasanmascheff, Hannes Mutschler   +4 more
wiley   +1 more source

Rapid Determination of Benzylpenicillin Resistance in Staphylococcus aureus Bacteraemia Model

Infection and Drug Resistance, 2020
JeongWoo Kang,1,2 Md Akil Hossain,1 Hae-chul Park,1 Yong-sang Kim,1 Sung-won Park,1 Tae-Wan Kim2 1Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency (APQA), Gimcheon-si, Gyeongsangbuk-do, Republic of Korea; 2Department of ...
Kang J, Hossain MA, Park H, Kim Y, Park S, Kim TW   +5 more
doaj  

Synthesis of Deuterated Methylphosphonic Acid Derivatives for Use as Standard Compounds in the Analysis of Toxic Chemicals

Вестник войск РХБ защиты, 2021
One of the ways to ensure high reliability of detection, identification and accuracy of quantitative determination of toxic chemicals (TC) in environmental and biomedical samples by gas and liquid chromatography-mass spectrometry methods is to use ...
V. А. Yashkir, V. I. Krylov, I. I. Krylov, A. V. Brown, D. V. Oreshkin, I. V. Rybalchenko, V. N. Fateenkov   +6 more
doaj   +1 more source

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source