Results 111 to 120 of about 1,750,612 (319)

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source

Biodegradable Hydrogel Pellets for Efficient Dye Adsorption: A Sustainable Approach to Industrial Effluent Treatment

Journal of Chemistry
Hydrogels are a special network of polymer chains capable of absorbing and retaining large volumes of water that are composed of naturally occurring polymers, synthetic polymers, or a combination of both.
Dhanalakshmi Vadivel, Nithishkumar Kameswaran, Daniele Dondi   +2 more
doaj   +1 more source

DNP-Supported Solid-State NMR Spectroscopy of Proteins Inside Mammalian Cells

, 2019
Elucidating at atomic level how proteins interact and are chemically modified in cells represents a leading frontier in structural biology. We have developed a tailored solid-state NMR spectroscopic approach that allows studying protein structure inside ...
Sub NMR Spectroscopy, Narasimhan, Siddarth, Folkers, Gert E., Paioni, A.L., Johan van der Zwan, Scherpe, Stephan, Ovaa, H., Marc Baldus, Gert E. Folkers, Baldus, Marc, NMR Spectroscopy, Scherpe, S., Folkers, G.E., Baldus, M., Alessandra Lucini Paioni, Narasimhan, S., Lucini Paioni, Alessandra, Stephan Scherpe, Huib Ovaa, van der Zwan, Johan, Siddarth Narasimhan, Zwan, J. van der, Ovaa, Huib   +22 more
core   +1 more source

SPECTROSCOPY OF CATALASE [PDF]

Journal of General Physiology, 1937
Catalase is resistant to oxidizing agents; e.g., ferricyanide. It is also resistant to reducing agents; e.g., catalytically activated hydrogen, hydrosulfite, ferrotartrate, cysteine. The hemin group of the enzyme will combine with cyanide, sulfides, nitric oxide, fluoride. It will not combine with carbon monoxide. Catalase is therefore a ferric complex.
openaire   +2 more sources

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Data-driven docking for the study of biomolecular complexes

, 2005
With the amount of genetic information available, a lot of attention has focused on systems biology, in particular biomolecular interactions. Considering the huge number of such interactions, and their often weak and transient nature, conventional ...
Boelens, Rolf, NMR Spectroscopy, Bonvin, Alexandre M. J. J., Sub NMR Spectroscopy, Van Dijk, Aalt D. J.   +4 more
core   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

NMR-based structural biology of microtubules and of microtubule-associated proteins

, 2019
The dynamic instability of microtubules (MT) is an essential feature in eukaryotic cells and related to basic cellular processes such as intracellular transport, cell division, migration, or polarization.
NMR Spectroscopy, Sub NMR Spectroscopy, Luo, Yanzhang   +2 more
core  

Label-free haemogram using wavelength modulated Raman spectroscopy for identifying immune-cell subset

, 2014
The authors thank the UK EPSRC the CR-UK/EPSRC/MRC/DoH (England) imaging Program and the European Union project FAMOS (FP7 ICT, contract no. 317744) for funding, K.D. is a Royal Society-Wolfson Merit Award Holder.Leucocytes in the blood of mammals form a
Bavishna B. Praveen, Powis, S.J., Campbell, E.C., Ashok, P.C., Praveen, B.B., Simon J. Powis, Dholakia, K., Praveen C. Ashok, Elaine C. Campbell, Kishan Dholakia   +9 more
core   +1 more source