Results 91 to 100 of about 1,750,612 (319)

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Array detection enables large localization range for simple and robust MINFLUX

Light: Science & Applications
The MINFLUX concept significantly improves the localization properties of single-molecule localization microscopy (SMLM) by overcoming the limit imposed by the fluorophore’s photon counts.
Eli Slenders, Sanket Patil, Marcus Oliver Held, Alessandro Zunino, Giuseppe Vicidomini   +4 more
doaj   +1 more source

Generation of 22-mJ, 2.0-ps Pulses from a 1-kHz Ho:YLF Regenerative Chirped Pulse Amplifier [PDF]

EPJ Web of Conferences
We report a CW-pumped Ho:YLF regenerative amplifier (RA) delivering pulses with 22.5-mJ energy and 2.0-ps duration at 1 kHz. The RA emitting at 2051 nm is broadband-seeded and implemented in a chirped pulse amplification system. © 2024 The Author(s)
Bock Martin, von Grafenstein Lorenz, Mero Mark, Nagy Tamas, Griebner Uwe   +4 more
doaj   +1 more source

1H, 13C and 15N resonance assignments of wild-type Bacillus subtilis Lipase A and its mutant evolved towards thermostability

, 2013
Previously, we evolved Lipase A from Bacillus subtilis towards increased thermostability. The resulting mutant retains significant catalytic activity upon heating above 60 C (and up to 100 C) and cooling down, whereas wild-type lipase precipitates ...
Reetz, M.T., NMR Spectroscopy, Augustyniak, W., Sub NMR Spectroscopy, Wienk, H., Boelens, R., Reetz, M., Wienk, H.L.J.   +7 more
core   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

In situ and real-time ultrafast spectroscopy of photoinduced reactions in perovskite nanomaterials

Nature Communications
By employing two synchronized mode-locked femtosecond lasers and interferometric detection of the pump-probe spectra—referred to as asynchronous and interferometric transient absorption—we have developed a method for broad dynamic range and rapid data ...
Gi Rim Han, Mai Ngoc An, Hyunmin Jang, Noh Soo Han, JunWoo Kim, Kwang Seob Jeong, Tai Hyun Yoon, Minhaeng Cho   +7 more
doaj   +1 more source

Structural and time-domain peculiarities of the fluorescence excitation spectra of single Mg-TAP in a polymer at low temperatures

EPJ Web of Conferences, 2018
The results of low-temperature measurements of spectral trails (temporal evolutions of individual fluorescence excitation spectra) of single molecules Mg-tetraazaporphyrin embedded in polyisobutylene are presented.
Savostianov Aleksandr, Eremchev Ivan, Gorshelev Alexei, Orlov Sergei, Korotayev Oleg, Naumov Andrei, Starukhin Aleksandr   +6 more
doaj   +1 more source

Shock Waves in Laser-Induced Plasmas

Atoms, 2019
The production of a plasma by a pulsed laser beam in solids, liquids or gas is often associated with the generation of a strong shock wave, which can be studied and interpreted in the framework of the theory of strong explosion.
Beatrice Campanella, Stefano Legnaioli, Stefano Pagnotta, Francesco Poggialini, Vincenzo Palleschi   +4 more
doaj   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Temperature Dependences of Single Dye-Molecules Zero-Phonon Line Widths in a Broad Range of Low Temperatures

EPJ Web of Conferences, 2015
In the present work the individual temperature dependences of zero-phonon spectral line widths of single tetra-tert-butylterrylene dye-molecules in polyisobutylene were measured in a broad range of low temperatures (from 20 K up to 67 K).
Eremchev I. Yu., Gorshelev A.A., Karimullin K.R., Naumov A.V.   +3 more
doaj   +1 more source