Forensic applications of raman spectroscopy [PDF]
The forensic applications of Raman spectroscopy have been explored and extended using the development of novel sampling techniques and task-specific instrumentation described in this thesis.
Thomson, Grant
core +4 more sources
Superresolution concentration measurement realized by sub-shot-noise absorption spectroscopy
Here, the authors use entangled photon pairs as the light source for absorption spectroscopy and demonstrate sub-shot-noise spectra in the entire visible wavelength region.
Korenobu Matsuzaki, Tahei Tahara
doaj +1 more source
Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics
Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt +8 more
wiley +1 more source
1H, 13C and 15N resonance assignments of wild-type Bacillus subtilis Lipase A and its mutant evolved towards thermostability [PDF]
Previously, we evolved Lipase A from Bacillus subtilis towards increased thermostability. The resulting mutant retains significant catalytic activity upon heating above 60 C (and up to 100 C) and cooling down, whereas wild-type lipase precipitates ...
Reetz, M.T. +7 more
core +1 more source
Versatile methods for the quantification of intracellular cholesterol are essential for understanding cellular physiology and for diagnosing disorders linked to cholesterol metabolism.
Enrico Baria +8 more
doaj +1 more source
Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang +2 more
wiley +1 more source
Measurements of collisional broadening coefficients by infrared polarization spectroscopy
We present measurements of collisional broadening coefficients, obtained at atmospheric pressure, by polarization spectroscopy. Using tunable single mode laser radiation at approximately 2 microm, high-resolution infrared polarization spectra were ...
Zetterberg, Johan, +11 more
core +1 more source
Removal of slow-pulsing artifacts in in-phase 15N relaxation dispersion experiments using broadband 1H decoupling [PDF]
Understanding of the molecular mechanisms of protein function requires detailed insight into the conformational landscape accessible to the protein. Conformational changes can be crucial for biological processes, such as ligand binding, protein folding ...
NMR Spectroscopy +4 more
core +1 more source
Formation of the β-barrel assembly machinery complex in lipid bilayers as seen by solid-state NMR
The β-barrel assembly machinery (BAM) catalyzes β-barrel protein insertion into the outer membrane of E.coli. Here authors employ high-sensitivity solid-state NMR to reveal how the lipid environment and formation of the BamA-BamCDE complex affect BamA ...
Cecilia Pinto +5 more
doaj +1 more source
A eukaryotic cell is a microscopic world within which efficient material transport is essential. Yet, how a cell manages to deliver cellular cargos efficiently in a crowded environment remains poorly understood.
Jin-Sung Park +4 more
doaj +1 more source

