Results 51 to 60 of about 10,311 (264)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Design and implementation of a waterless solar panel cleaning system

Unconventional Resources
Manual cleaning of large solar installations is often labor-intensive and time-consuming, primarily due to the accumulation of dust on solar panels, which significantly impairs their efficiency.
Charity M. Nkinyam, Chika O. Ujah, Kingsley C. Nnakwo, Obiora Ezeudu, Daramy V.V. Kallon, Ikechukwu Ike-Eze C. Ezema   +5 more
doaj   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Advances in MoS2-Based ternary nanocomposites for high-performance electrochemical energy storage

Hybrid Advances
The field of rechargeable energy storage devices is actively exploring nanostructured materials with intricate morphologies due to several advantages. The integration of nanostructured materials into various electrodes, and rechargeable systems holds the
Seyoum A. Getaneh, Abdudin G. Temam, Getachew A. Workneh, Assumpta C. Nwanya, Paul M. Ejikeme, Fabian I. Ezema   +5 more
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

To make SPED a success

, 2012
This is a short letter published in the Mediacorp tabloid "Today" under the 'Voices' section , p. 12, on 12 November 2012. It touches on the writer's suggestion how to make Special Education (SPED) a success in Singapore: what to expect in more positive contructive engagement and positive changes in the SPED landscape in Singapore.  
openaire   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Taxonomia XBRL-SPED: simplificação e auditoria no âmbito do Sistema Público de Escrituração Digital (SPED)

iSys - Brazilian Journal of Information Systems, 2018
Empresas necessitam de dados operacionais, financeiros e não financeiros que possuam credibilidade, confiabilidade, integridade e disponibilidade para os processos decisórios e para o intercâmbio via web. Extrair e processar dados de fontes diversas não é tarefa trivial.
José Geraldo Luciano, Paulo Caetano da Silva, Clóvis Belbute Peres   +2 more
openaire   +2 more sources

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source