Results 61 to 70 of about 10,333 (261)

Taxonomia XBRL-SPED: simplificação e auditoria no âmbito do Sistema Público de Escrituração Digital (SPED)

iSys - Brazilian Journal of Information Systems, 2018
Empresas necessitam de dados operacionais, financeiros e não financeiros que possuam credibilidade, confiabilidade, integridade e disponibilidade para os processos decisórios e para o intercâmbio via web. Extrair e processar dados de fontes diversas não é tarefa trivial.
José Geraldo Luciano, Paulo Caetano da Silva, Clóvis Belbute Peres   +2 more
openaire   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

User-Centric Cloud RAN: An Analytical Framework for Optimizing Area Spectral and Energy Efficiency

IEEE Access, 2018
In this article, we develop a statistical framework to quantify the area spectral efficiency (ASE) and the energy efficiency (EE) performance of a user-centric cloud based radio access network (UC-RAN) downlink.
Umair Sajid Hashmi, Sped Ali Raza Zaidi, Ali Imran   +2 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

PERCEPÇÃO DOS CONTADORES QUANTO ÀS DIFICULDADES DE COMPETÊNCIA TÉCNICA PARA A IMPLANTAÇÃO DO SPED NAS ENTIDADES DO TERCEIRO SETOR, EM CUMPRIMENTO À INSTRUÇÃO NORMATIVA Nº 1.510/2014

Revista Ambiente Contábil, 2018
O objetivo deste estudo é analisar a percepção dos contadores quanto às dificuldades de competência técnica para a implantação do Sistema Público de Escrituração Digital (SPED) nas entidades do terceiro setor.
Amanda de Lima Marques da Silva, Elayne Patrícia Ribeiro de Santana Azevedo, Ana Lúcia Fontes de Souza Vasconcelos, Márcia Ferreira Neves Tavares, Marcelo Jota Gomes   +4 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Development of solar photovoltaic as the mainstream source of energy in the fourth industrial revolution (4IR)

International Journal of Thermofluids
The fourth industrial revolution (4IR) era is characterized by the adoption of Innovative and emerging renewable technologies, such as solar photovoltaic (PV), which are pivotal in driving the transition to green energy. This study examines the synergies
Charity Mokom, Chika Oliver Ujah, Christian O. Asadu, Peter A. Olubambi   +3 more
doaj   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

FEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks, Garth L. Maker, Emily C. Davies, Berin A. Boughton, Samantha Lodge   +4 more
wiley   +1 more source