Results 61 to 70 of about 17,993 (239)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

A ESTRUTURA DO SISTEMA PÚBLICO DE ESCRITURAÇÃO DIGITAL (SPED): um estudo das novas obrigações

Navus: Revista de Gestão e Tecnologia, 2012
O Sistema Público de Escrituração Digital (SPED) é um instrumento de acompanhamento e fiscalização criado pelo governo, cujo principal objetivo é promover a integração dos fiscos, mediante a padronização e compartilhamento das informações contábeis e ...
LIZOTE, Suzete Antonieta, MARIOT, Diego Menin   +1 more
doaj   +3 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Out-of-field Teaching: English as SPED Teachers Lived Experiences [PDF]

Out-of-field teaching remains a persistent issue in education, especially when teachers are assigned to teach and support students with disabilities in Special Education (SPED) classrooms without the necessary training or qualifications.
R. Amodia, Daphil Mae, P. Calesa, Kith Bryan, Bacus, Remedios C., Segubia, Angela G., Juguilon, Grazella Mae C.   +4 more
core   +2 more sources

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Prediction of Nanoscale Grain-Boundary Migration in Pure Aluminum Through Assimilation of Time-Resolved TEM-SPED Orientation Maps

Journal of Manufacturing and Materials Processing
Scanning precession electron diffraction in transmission electron microscopy (TEM-SPED) enables highly accurate nanoscale orientation mapping and facilitates time-resolved tracking of microstructural changes, including grain boundary migration in ...
Yuyao Zhu, Shiro Ihara, Mitsuhiro Murayama   +2 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Design and implementation of a waterless solar panel cleaning system

Unconventional Resources
Manual cleaning of large solar installations is often labor-intensive and time-consuming, primarily due to the accumulation of dust on solar panels, which significantly impairs their efficiency.
Charity M. Nkinyam, Chika O. Ujah, Kingsley C. Nnakwo, Obiora Ezeudu, Daramy V.V. Kallon, Ikechukwu Ike-Eze C. Ezema   +5 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source