Results 61 to 70 of about 10,311 (264)

Implementation Planning: SPED Referral Flowchart

open access: yes, 2021
No description available.
Haßler, Björn   +3 more
openaire   +2 more sources

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

open access: yesFEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks   +4 more
wiley   +1 more source

PERCEPÇÃO DOS CONTADORES QUANTO ÀS DIFICULDADES DE COMPETÊNCIA TÉCNICA PARA A IMPLANTAÇÃO DO SPED NAS ENTIDADES DO TERCEIRO SETOR, EM CUMPRIMENTO À INSTRUÇÃO NORMATIVA Nº 1.510/2014

open access: yesRevista Ambiente Contábil, 2018
O objetivo deste estudo é analisar a percepção dos contadores quanto às dificuldades de competência técnica para a implantação do Sistema Público de Escrituração Digital (SPED) nas entidades do terceiro setor.
Amanda de Lima Marques da Silva   +4 more
doaj   +1 more source

Fluid Biomarkers of Disease Burden and Cognitive Dysfunction in Progressive Supranuclear Palsy

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Identifying objective biomarkers for progressive supranuclear palsy (PSP) is crucial to improving diagnosis and establishing clinical trial and treatment endpoints. This study evaluated fluid biomarkers in PSP versus controls and their associations with regional 18F‐PI‐2620 tau‐PET, clinical, and cognitive outcomes.
Roxane Dilcher   +10 more
wiley   +1 more source

User-Centric Cloud RAN: An Analytical Framework for Optimizing Area Spectral and Energy Efficiency

open access: yesIEEE Access, 2018
In this article, we develop a statistical framework to quantify the area spectral efficiency (ASE) and the energy efficiency (EE) performance of a user-centric cloud based radio access network (UC-RAN) downlink.
Umair Sajid Hashmi   +2 more
doaj   +1 more source

Structure–Function Decoupling of the Sensorimotor and Default Mode Networks in Black Americans With MS

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Background and Objectives Multiple sclerosis (MS) exhibits racially disparate rates of disease progression. Black people with MS (B‐PwMS) experience a more severe disease course than non‐Hispanic White people with MS (NHW‐PwMS). Here we investigated structural and functional connectivity as well as structure–function decoupling in the ...
Emilio Cipriano   +11 more
wiley   +1 more source

Development of solar photovoltaic as the mainstream source of energy in the fourth industrial revolution (4IR)

open access: yesInternational Journal of Thermofluids
The fourth industrial revolution (4IR) era is characterized by the adoption of Innovative and emerging renewable technologies, such as solar photovoltaic (PV), which are pivotal in driving the transition to green energy. This study examines the synergies
Charity Mokom   +3 more
doaj   +1 more source

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