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Sperm Cryopreservation Methods

2012
There are multiple clinical situations where cryopreservation of sperm is required including sperm banking prior to chemotherapy or radiation treatment for cancer, donor sperm for couples without a male partner, and various etiologies of male factor infertility. Bunge and Sherman presented the first pregnancy resulting from the thawing of cryopreserved
Tiffany, Justice, Greg, Christensen
openaire   +2 more sources

Zebrafish Sperm Cryopreservation

2004
Publisher Summary This chapter reviews the important elements that should be considered when developing a sperm cryopreservation protocol in zebrafish. It also presents an alternative freezing method and discusses further considerations and future directions for the continued optimization of zebrafish cryopreservation. The goal of cryopreservation is
Stéphane, Berghmans   +3 more
openaire   +2 more sources

Cryopreservation of echinoderm sperm

Canadian Journal of Zoology, 1973
A simple method for the cryopreservation of sea urchin, sand dollar, and starfish sperm is described. Using dimethyl sulfoxide (DMSO) as the protective agent, sperm were stored up to 30 days at — 196 °C. These preparations all retained some motility after thawing.
R S, Dunn, J, McLachlan
openaire   +2 more sources

Cryopreservation of human sperm

Human Fertility, 2001
Freezing of human sperm is considered a routine procedure in assisted reproductive technology (ART) laboratories. This article considers various aspects of cryopreservation of human sperm. Human sperm show a specific cryophysical behaviour and different sperm freezing protocols have been developed to avoid damage to the sperm cells.
M, Nijs, W, Ombelet
openaire   +2 more sources

Cryopreservation of Pooled Sperm Samples

2021
Cryopreservation of sperm cells is currently the most efficient tool for managing large and small collections of valuable genetic resources. Cryopreservation minimizes expenses for animal and facility maintenance such as personnel, water, power, and space.
Jennifer L, Matthews, Zoltan M, Varga
openaire   +2 more sources

Cryopreservation and Sperm DNA Integrity

Cell and Tissue Banking, 2006
Cryopreservation of sperm is an extremely important issue in the field of male infertility as freezing can have detrimental effects on a variety of sperm functions, some of them not accessible to the traditional semen quality analysis. In this study, chromatin structure variations in human spermatozoa in semen were studied with the sperm chromatin ...
GANDINI, Loredana   +4 more
openaire   +3 more sources

Rabbit Sperm Cryopreservation

Sperm cryopreservation is an excellent way to store genetic materials for a long period. Semen cryopreservation is associated with structural and functional damage of the spermatozoa caused by cold shock, osmotic stress, intracellular ice crystal formation, and the production of reactive oxygen species, which reduce their functional lifespan and ...
Maria Pilar, Viudes de Castro   +2 more
openaire   +2 more sources

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