Vitrification Ability of Combined and Single Cryoprotective Agents [PDF]
Plants, 2021 Cryoprotective agents (CPA) are an important part of many current vitrification methods. The vitrification ability of CPAs influences the probability of the glass transition and water crystallization occurrence.
Milos Faltus +2 more
doaj +6 more sources
Natural Cryoprotective and Cytoprotective Agents in Cryopreservation: A Focus on Melatonin
Molecules, 2022 Cryoprotective and cytoprotective agents (Cytoprotective Agents) are fundamental components of the cryopreservation process. This review presents the essentials of the cryopreservation process by examining its drawbacks and the role of cytoprotective ...
Giada Marcantonini +10 more
doaj +6 more sources
Polyampholytes as Cryoprotective Agents for Mammalian Cell Cryopreservation [PDF]
Cell Transplantation, 2010 Cryoprotective agents (CPAs) such as dimethyl sulfoxide (DMSO), glycerol, ethylene glycol, and propylene glycol have been used for the cryopreservation of cells and tissues.
Kazuaki Matsumura +2 more
doaj +7 more sources
The Role of Cryoprotective Agents in Liposome Stabilization and Preservation. [PDF]
Int J Mol Sci, 2022 To improve liposomes’ usage as drug delivery vehicles, cryoprotectants can be utilized to prevent constituent leakage and liposome instability. Cryoprotective agents (CPAs) or cryoprotectants can protect liposomes from the mechanical stress of ice ...
Boafo GF +5 more
europepmc +4 more sources
Impact of cryoprotective agents on human gut microbes and in vitro stabilized artificial gut microbiota communities [PDF]
Microbial BiotechnologyThe availability of microbial biobanks for the storage of individual gut microbiota members or their derived and artificially assembled consortia has become fundamental for in vitro investigation of the molecular mechanisms behind microbe–microbe and/or ...
Giulia Alessandri +7 more
doaj +3 more sources
Ultrasonics Sonochemistry, 2023 Lactic acid bacteria (LAB) are being used for probiotic and starter cultures to prevent global damage to microbial cells. To retain the benefits of LAB in the commercially used powdered form, highly efficient cryoprotective agents are required during the
Yeong Yeol Kim +5 more
doaj +2 more sources
Vitrification for cryopreservation of 2D and 3D stem cells culture using high concentration of cryoprotective agents. [PDF]
BMC Biotechnol, 2020 Background Vitrification is the most promising technology for successful cryopreservation of living organisms without ice crystal formation. However, high concentrations (up to ~ 6–8 M) of cryoprotective agents (CPAs) used in stem cell induce osmotic and
Jeong YH +9 more
europepmc +3 more sources
Loading equine oocytes with cryoprotective agents captured with a finite element method model. [PDF]
Sci Rep, 2021 Cryopreservation can be used to store equine oocytes for extended periods so that they can be used in artificial reproduction technologies at a desired time point.
Içli S +5 more
europepmc +2 more sources
Cryopreservation of Endothelial Cells in Various Cryoprotective Agents and Media - Vitrification versus Slow Freezing Methods. [PDF]
PLoS One, 2016 Vitrification of endothelial cells (MHECT-5) has not previously been compared with controlled slow freezing methods under standardized conditions. To identify the best cryopreservation technique, we evaluated vitrification and standardized controlled ...
von Bomhard A +4 more
europepmc +3 more sources
Tailoring Freeze-Drying for Starter Cultures Preservation: A Case Study with Lactiplantibacillus plantarum [PDF]
FoodsFreeze-drying is a crucial technique for preserving bacterial strains, yet its efficiency depends heavily on the precise selection of protective agents.
Antoni Miecznikowski +3 more
doaj +2 more sources