Vitrification Ability of Combined and Single Cryoprotective Agents. [PDF]
Plants (Basel), 2021 Cryoprotective agents (CPA) are an important part of many current vitrification methods. The vitrification ability of CPAs influences the probability of the glass transition and water crystallization occurrence. Thermal characteristics and the vitrification ability of two combined CPAs (PVS2 and PVS3), common plant vitrification solutions, and four ...
Faltus M, Bilavcik A, Zamecnik J.
europepmc +5 more sources
Cryopreservation of equine oocytes: looking into the crystal ball [PDF]
, 2020 In vitro embryo production has evolved rapidly in the horse over the past decade, but blastocyst rates from vitrified equine oocytes remain quite poor and further research is needed to warrant application.
Angel Velez, Daniel +4 more
core +2 more sources
Combinatorial biomaterials discovery strategy to identify new macromolecular cryoprotectants [PDF]
, 2020 Cryoprotective agents (CPAs) are typically solvents or small molecules, but there is a need for innovative CPAs to reduce toxicity and increase cell yield, for the banking and transport of cells.
Gibson, Matthew I. +4 more
core +2 more sources
Polyampholytes as emerging macromolecular cryoprotectants [PDF]
, 2020 Cellular cryopreservation is a platform technology which underpins cell biology, biochemistry, biomaterials, diagnostics, and the cold chain for emerging cell-based therapies.
Bailey, Trisha L. +3 more
core +2 more sources
Pressure in isochoric systems containing aqueous solutions at subzero Centigrade temperatures. [PDF]
, 2017 ObjectivePreservation of biological materials at subzero Centigrade temperatures, cryopreservation, is important for the field of tissue engineering and organ transplantation.
Năstase, Gabriel +3 more
core +6 more sources
Cryopreservation: Methods, equipment and critical concerns [PDF]
, 2016 The technique of cryopreservation aims to maintain the biological samples in a metabolic suspension state, by which all chemical, physical and biological changes are diminished.
Herrmann, J +3 more
core +1 more source
Polyampholytes as Cryoprotective Agents for Mammalian Cell Cryopreservation [PDF]
Cell Transplantation, 2010 Cryoprotective agents (CPAs) such as dimethyl sulfoxide (DMSO), glycerol, ethylene glycol, and propylene glycol have been used for the cryopreservation of cells and tissues. DMSO is the most effective CPA but shows high cytotoxicity and can effect differentiation.
Kazuaki Matsumura +2 more
openaire +4 more sources
Stepped vitrification technique for human ovarian tissue cryopreservation [PDF]
, 2019 The advantage of stepped vitrification (SV) is avoiding ice crystal nucleation, while decreasing the toxic effects of high cryoprotectant concentrations. We aimed to test this method for human ovarian tissue cryopreservation.
Amorin, C.A. +9 more
core +1 more source
Cryopreservation of swine ovarian tissue : effect of different cryoprotectants on the structural preservation of preantral follicle oocytes [PDF]
, 2009 The present study aimed to test different cryoprotectants on cryopreservation of pig ovarian tissue. Pig ovaries (n = 3) were collected at a local slaughterhouse. From each ovary, ten cortex samples were taken.
Amorima, C. A. +6 more
core +1 more source
Toxicity of cryoprotective agents at 30°
Journal of Pharmacy and Pharmacology, 1968 Abstract The toxicity of four cryoprotective agents (glycerol, dimethylsulfoxide, dextran, magnesium ion) as a function of time was assessed at 30° on isolated rabbit atria. Glycerol, 2·0 M, in Ringer solution killed all atria in less than 30 min. Atria tolerated 2·1 M dimethylsulphoxide in Ringer for 1 hr, 0·7 M glycerol in Ringer for 4
Armand M Karow +2 more
openaire +1 more source