Results 21 to 30 of about 1,545,419 (198)

Cellular cryobiology - a review of basic concepts and "operating-design" of cryopreserved cells [PDF]

open access: yesVojnosanitetski Pregled, 2023
nema
Balint Bela   +6 more
doaj   +1 more source

Insights into Chemical Interactions and Related Toxicities of Deep Eutectic Solvents with Mammalian Cells Observed Using Synchrotron Macro–ATR–FTIR Microspectroscopy

open access: yesBiophysica, 2023
Deep eutectic solvents (DESs) and ionic liquids (ILs) are highly tailorable solvents that have shown a lot of promise for a variety of applications including cryopreservation, drug delivery, and protein stabilisation.
Saffron J. Bryant   +8 more
doaj   +1 more source

Cryopreservation of Endothelial Cells in Various Cryoprotective Agents and Media - Vitrification versus Slow Freezing Methods. [PDF]

open access: yesPLoS One, 2016
Vitrification of endothelial cells (MHECT-5) has not previously been compared with controlled slow freezing methods under standardized conditions. To identify the best cryopreservation technique, we evaluated vitrification and standardized controlled ...
von Bomhard A   +4 more
europepmc   +4 more sources

Carboxylated Poly-l-Lysine as a Macromolecular Cryoprotective Agent Enables the Development of Defined and Xeno-Free Human Sperm Cryopreservation Reagents

open access: yesCells, 2021
In human sperm cryopreservation, test yolk buffer and human serum albumin have been used as permeating macromolecular-weight cryoprotectants. In clinical reproductive medicine, human serum albumin is frequently used because of low risks of zoonoses and ...
Hiroki Takeuchi   +13 more
doaj   +1 more source

Caffeic Acid Phenethyl Ester Alleviates Cryodamage To Lung Cancer Cells During Cryopreservation

open access: yesHittite Journal of Science and Engineering, 2021
Cryopreservation is widely used technique for long-term preservation of viable cells at low temperature. In this process, considering the effects of cryodamage on cells, the application of safe and efficient cryoprotective agents is very important ...
Ezgi Avşar Abdik
doaj   +1 more source

Aquaporins and Animal Gamete Cryopreservation: Advances and Future Challenges

open access: yesAnimals, 2022
Cryopreservation is globally used as a method for long-term preservation, although freeze-thawing procedures may strongly impair the gamete function.
João C. Ribeiro   +4 more
doaj   +1 more source

Rapid joule heating improves vitrification based cryopreservation

open access: yesNature Communications, 2022
Lower concentrations of toxic cryoprotective agents require rapid and scalable rewarming techniques. Here, the authors report on a joule heating–based platform where samples are rapidly rewarmed by contact with a voltage pulsed electrical conductor and ...
Li Zhan   +5 more
doaj   +1 more source

Yeast Exoglycoproteins Produced Under NaCl-Stress Conditions as Efficient Cryoprotective Agents

open access: yesBiological Procedures Online, 1998
Six extracellular yeast glycoproteins were prepared from three yeast species in osmotic equilibrium and unequilibrium environments and used as non-penetrating cryoadditives. Glycoproteins secreted by the strain Dipodascus australiensis into growth medium
Breierova Emilia
doaj   +1 more source

Optimization of Adipose Tissue Cryopreservation Techniques in a Murine Model

open access: yesPlastic and Reconstructive Surgery, Global Open, 2021
Background:. The aim of this study was to develop an adipose tissue (AT) cryopreservation protocol that is effective, simple, and maintains the functionality and viability of AT after thawing and transplantation. Methods:.
María Eloísa Villaverde-Doménech, PhD   +5 more
doaj   +1 more source

The effect of glycerol as a cryoprotective agent in the cryopreservation of adipose tissue

open access: yesStem Cell Research & Therapy, 2021
Abstract Background Long-term preservation of adipose tissue is crucial for clinical applications. Researchers should consider both efficiency and biosafety when choosing a cryoprotective agent (CPA) for adipose tissue preservation.
Pei-Qi Zhang   +7 more
openaire   +3 more sources

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