Results 11 to 20 of about 3,957 (196)

A Dilution-Filtration System for Removing Cryoprotective Agents

Journal of Biomechanical Engineering, 2011
In most cryopreservation applications, the final concentrations of cryoprotective agents (CPAs) must be reduced to biocompatible levels. However, traditional methods for removing CPAs usually have disadvantages of operation complexity, time consumption ...
Xiaoming Zhou, Zhong Liu, Zhiquan Shu
exaly   +4 more sources

Deep eutectic solvents as cryoprotective agents for mammalian cells

Journal of Materials Chemistry B, 2022
Cryopreservation has facilitated numerous breakthroughs including assisted reproductive technology, stem cell therapies, and species preservation. Successful cryopreservation requires the addition of cryoprotective agents to protect against freezing ...
Saffron J Bryant, Miyah N Awad, Aaron Elbourne   +2 more
exaly   +4 more sources

Toxicity of cryoprotective agents at 30°

Journal of Pharmacy and Pharmacology, 1968
The toxicity of four cryoprotective agents (glycerol, dimethylsulfoxide, dextran, magnesium ion) as a function of time was assessed at 30° on isolated rabbit atria. Glycerol, 2·0 M, in Ringer solution killed all atria in less than 30 min. Atria tolerated
Armand M Karow, Oliver Carrier, Ben R Clower   +2 more
core   +2 more sources

Cryopreservation of cells using defined serum-free cryoprotective agents

Current Directions in Biomedical Engineering, 2016
For regenerative purposes, there is a high demand for viable and active cells. A big issue is to have enough viable cells available at any given time. One solution is cryopreservation.
Volbers Jan-Cedric, Lauterböck Lothar, Hofmann Nicola, Glasmacher Birgit   +3 more
doaj   +4 more sources

Permeability and Toxicity of Cryoprotective Agents in Silkworm Embryos: Impact on Cryopreservation. [PDF]

Int J Mol Sci
The permeation of cryoprotectants into insect embryos is critical for successful cryopreservation. However, the permeability of silkworm embryos to cryoprotectants and the effects of cryopreservation remain poorly studied. In this study, we evaluated the
Urbán-Duarte D, Tomita S, Sakai H, Sezutsu H, Álvarez-Gallardo H, Kainoh Y, Furukawa S, Uchino K.   +7 more
europepmc   +2 more sources

Synthetic Polyampholytes as Macromolecular Cryoprotective Agents

Langmuir, 2018
A series of polyampholytes based on different molar ratios on N,N-dimethylaminopropyl methacrylamide (DMAPMA), acrylic acid (AA), and optionally, N-tert-butylacrylamide (t-BuAAm), were prepared by free radical copolymerization, and tested as DMSO-free ...
R. Fisher, J. Zhao, N. A. D. Burke, N. A. D. Burke (5814218), M. A. Johnson, H. D. H. Stöver, R. Fisher (5814215), H. D. H. Stöver (5814221), J. Zhao (2086252), M. A. Johnson (2337901)   +9 more
core   +3 more sources

Ultra-Fast Vitrification: Minimizing the Toxicity of Cryoprotective Agents and Osmotic Stress in Mouse Oocyte Cryopreservation. [PDF]

Int J Mol Sci
Globally, women have been adopting oocyte cryopreservation (OC) for fertility preservation for various reasons, such as inevitable gonadotoxic treatment for specific pathologic states and social preferences.
Cho JR, Yu EH, Lee HJ, Kim IH, Jeong JH, Lee DB, Cho SK, Joo JK.   +7 more
europepmc   +2 more sources

CELT^PLUS Fat Increases the Metabolic Activity as Well as the SVF-Yield Significantly When Compared to CELT Fat, Even After Cryopreservation with DMSO [PDF]

Cells
Lipofilling has far more applications than cosmetic surgery alone. Due to its high content of stromal vascular fraction (SVF) cells, lipoaspirate can also be used to treat wounds, as its cellular components may accelerate wound healing.
Tom Schimanski, Lukas Prantl, Andreas Eigenberger, Oliver Felthaus, Rafael Loucas, Kirsten Utpatel, Kerstin Steer   +6 more
doaj   +2 more sources

A circular exploration of cryoprotective agents for stem cells using computer-aided molecular design approaches

Digital Chemical Engineering
This work presents a circular exploration of cryoprotective agents (CPAs) for stem cells using computer-aided molecular design approaches that can comprehensively consider compounds.
Rei Tamaki, Yusuke Hayashi, Yuki Uno, Masahiro Kino-oka, Hirokazu Sugiyama   +4 more
doaj   +2 more sources

Cryopreservation of ovarian tissue – what's known so far and future perspectives [PDF]

Frontiers in Reproductive Health
BackgroundCryopreservation is widely used across the life sciences to enable long-term storage of living cells and tissues for research or later clinical use. Its core principle is the arrest of biological activity at extremely low temperatures.
Sabine Eberhart, Hazem Khalifa, Laura Rafensteiner, Josef Lehner, Katharina Hancke, Karin Bundschu   +5 more
doaj   +2 more sources