Results 11 to 20 of about 1,545,419 (198)
Polyampholytes as Cryoprotective Agents for Mammalian Cell Cryopreservation [PDF]
Cell Transplantation, 2010 Cryoprotective agents (CPAs) such as dimethyl sulfoxide (DMSO), glycerol, ethylene glycol, and propylene glycol have been used for the cryopreservation of cells and tissues.
Kazuaki Matsumura +2 more
doaj +6 more sources
The Role of Cryoprotective Agents in Liposome Stabilization and Preservation. [PDF]
Int J Mol Sci, 2022 To improve liposomes’ usage as drug delivery vehicles, cryoprotectants can be utilized to prevent constituent leakage and liposome instability. Cryoprotective agents (CPAs) or cryoprotectants can protect liposomes from the mechanical stress of ice by vitrifying at a specific temperature, which forms a glassy matrix.
Boafo GF +5 more
europepmc +4 more sources
Toxicity of cryoprotective agents at 30°
Journal of Pharmacy and Pharmacology, 1968 Abstract The toxicity of four cryoprotective agents (glycerol, dimethylsulfoxide, dextran, magnesium ion) as a function of time was assessed at 30° on isolated rabbit atria. Glycerol, 2·0 M, in Ringer solution killed all atria in less than 30 min. Atria tolerated 2·1 M dimethylsulphoxide in Ringer for 1 hr, 0·7 M glycerol in Ringer for 4
Armand M Karow +2 more
openaire +2 more sources
Synthetic Polyampholytes as Macromolecular Cryoprotective Agents
Langmuir, 2018 A series of polyampholytes based on different molar ratios on N, N-dimethylaminopropyl methacrylamide (DMAPMA), acrylic acid (AA), and optionally, N- tert-butylacrylamide ( t-BuAAm), were prepared by free radical copolymerization, and tested as DMSO-free cryoprotective agents for 3T3 fibroblast cells by using a standard freeze-rethaw protocol ...
J. Zhao +4 more
openaire +3 more sources
Digital Chemical EngineeringThis work presents a circular exploration of cryoprotective agents (CPAs) for stem cells using computer-aided molecular design approaches that can comprehensively consider compounds.
Rei Tamaki +4 more
doaj +2 more sources
Research Progresses in Vitrification by Minimum Volume Method
Zhileng xuebao, 2018 Vitrification is an effective method in the field of cryopreservation since it can prevent the formation of ice crystals. Vitrification requires ultra-fast cooling rates and high concentrations of cryoprotective agents.
Zhang Xiaomin, Zhou Xinli
doaj +2 more sources
Analysis of Thermal and Crystallization in Cryoprotective Agents
Zhileng xuebao, 2014 Ice crystal formation is one of the factors that causes cell damage during crvopreservation process．Ice crystal will provide fundamental information for the development of appropriate cryopreservation protocols．Effects of concentration and properties of ...
Yang Bo +3 more
doaj +1 more source
Vitrification for cryopreservation of 2D and 3D stem cells culture using high concentration of cryoprotective agents. [PDF]
BMC Biotechnol, 2020 Background Vitrification is the most promising technology for successful cryopreservation of living organisms without ice crystal formation. However, high concentrations (up to ~ 6–8 M) of cryoprotective agents (CPAs) used in stem cell induce osmotic and
Jeong YH +9 more
europepmc +3 more sources
Modification of cellular membranes conveys cryoprotection to cells during rapid, non-equilibrium cryopreservation. [PDF]
PLoS ONE, 2018 Rapid cooling and re-warming has been shown promising to cryopreserve living cells, which cannot be preserved by conventional slow freezing methods. However, success is limited by the cytotoxicity of highly concentrated cryoprotective agents.
Jan Huebinger
doaj +1 more source
Effect of antifreeze on the quality of cooked crawfish tail meat after freeze-thawed
Shipin yu jixie, 2023 Objective: The study aimed to improve the storage quality of frozen cooked crawfish tail meat. Methods: Crawfish tail meat were immersed in different proportions of complex phosphate, trehalose and sorbitol antifreeze agents. The thawing loss rate, water
HU Xiao-bo +5 more
doaj +1 more source