Results 11 to 20 of about 7,269 (173)

A Cryoprotectant‐Compatible Nanoporous Platform for Stable and Scalable Delivery of Biopharmaceuticals

Advanced Materials, EarlyView.
A cryoprotectant‐compatible nanoporous platform enables ambient‐stable and scalable delivery of gene editing therapeutics. By combining hierarchical pore architecture with optimized lyophilization chemistry, the system preserves Cas9‐RNP activity post‐freeze‐drying.
Sian Lee, Seongchan Kim, Jooyeun Chong, Min‐gyeong Kim, Kayeong Lim, Nan‐Ee Lee, Jahyun Koo, Jiheong Kang, Hyojin Lee   +8 more
wiley   +1 more source

Kinsenoside Targets IDH1 to Restore Microglial Immune‐Metabolic Homeostasis for Alzheimer's Disease Therapy

Advanced Science, EarlyView.
Dysregulated TCA cycle contributes to Alzheimer's disease (AD) pathogenesis. Here, we show that microglial isocitrate dehydrogenase 1 (IDH1) is a critical driver. Elevated IDH1 disrupts citrate metabolism and mitochondrial function, exacerbating AD pathology.
Qianqian Li, Yajin Liao, Yan‐bo Zhao, Hongxing Wu, Tong Jin, Shuoshuo Li, Yuhan Liu, Peng Li, Songying Ouyang, Zekai Li, YuTing Xia, Qian Hua, Rui‐Yuan Pan, Zengqiang Yuan   +13 more
wiley   +1 more source

Multidimensional and Multifunctional Laser‐Induced Graphene (LIG) for Point‐of‐Care and Wearable Biosensing, Theranostics, and Bioactive Interfaces Toward Personalized Healthcare and Regenerative Medicine

Advanced Science, EarlyView.
Multidimensional laser‐induced graphene (LIG) spanning from 0D to 3D architectures is comprehensively reviewed for multifunctional biomedical platforms, including biosensing, theranostics, and bioactive interface applications, which highlights its potentials for point‐of‐care diagnostics, wearable health monitoring, smart drug delivery, and tissue ...
Li Zhang, Yurou Yuan, Xiaohong Ding, Wing Cheung Mak   +3 more
wiley   +1 more source

Space biomedicine‐oriented bioink and biomimetic blood‒brain barrier

VIEW, EarlyView.
Bioinks and bioprinting play a pivotal role in the successful delivery of cell models and the fabrication of tissue models for space biomedicine research. This study investigated the effects of hypergravity and vibration on two‐dimensional adherent cells and three‐dimensional embedded cells and provided methods for storing hybrid bioink and high ...
Ranran Yan, Ge Gao, YuJuan Li, YuLin Deng   +3 more
wiley   +1 more source

Cold hardening and dehardening in Salix [PDF]

, 2003
The variation in cold hardiness in Salix in the autumn was investigated using clones of different geographic origins. In late growing season, the variation was small and inversely related to a phenotypic variation in potential growth rate.
Lennartsson, Mattias
core  

Improvement of Mouse Spermatozoa Freezing at ‐80°C With Ascorbic Acid 2‐Glucoside at Thawing Phase

Andrology, EarlyView.
ABSTRACT Background Sperm cryopreservation is the most common method to maintain a great number of mutant mouse lines. However, the use of liquid nitrogen (LN2) for freezing presents considerable problems in terms of cost, safety, and accessibility. For this reason, the storage of semen samples at ‐80° in ultra‐freezers has been increasingly used in ...
Alessia Paradiso, Renata Paoletti, Simone Cassetti, Nicola Bernabò, Barbara Barboni, Marcello Raspa, Ferdinando Scavizzi   +6 more
wiley   +1 more source

Preservation of bovine preantral follicle viability and ultra-structure after cooling and freezing of ovarian tissue [PDF]

, 2008
Bovine preantral follicles within ovarian fragments were exposed and cryopreserved in absence or presence of 1.5 M glycerol (GLY), ethylene glycol (EG), propanediol (PROH) or dimethyl sulfoxide (DMSO), undergoing a previous cooling at 20 °C for 1 h ...
Báo, Sônia Nair, Celestino, Juliana Jales de Hollanda, Figueiredo, José Ricardo de, Lopes, Cláudio Afonso Pinho, Martins, Fabrício Sousa, Matos, Maria Helena Tavares, Melo, Mônica Aline Parente, Rodrigues, Ana Paula Ribeiro, Santos, Regiane Rodrigues dos, Silva, José Roberto Viana   +9 more
core   +1 more source

Biopreservation of hepatocytes: current concepts on hypothermic preservation, cryopreservation, and vitrification [PDF]

, 2013
Isolated liver cells (primarily isolated hepatocytes) have found important applications in science and medicine over the past 40 years in a wide range of areas, including physiological studies, investigations on liver metabolism, organ preservation and ...
Balaban, Cecilia Lucía, Fuller, Barry J., Guibert, Edgardo Elvio, Petrenko, Alexander Y., Rodriguez, Joaquin Valentin, Somov, Alexander Y.   +5 more
core  

Serum‐derived albumin as a cryoprotective agent for the long‐term storage of red blood cells: A preliminary examination

Transfusion, EarlyView.
Abstract Background Cryopreservation extends the shelf life of red blood cells (RBC) from weeks to years, offering major advantages for blood banking logistics, especially in remote environments. However, current glycerol‐based methods, though effective, are limited in scope due to complex post‐thaw washing and incompatibility with routine clinical ...
Thomas Bailey‐Schmidt, Christine V. Saunders, Chloë E. George, Thomas G. Scorer, Lynn M. R. McCallum, Tracey E. Madgett   +5 more
wiley   +1 more source

Evaluation on Utilization of Small Marine Fish to Produce Surimi Using Different Cryoprotective Agents to Increase the Quality of Surimi [PDF]

, 2008
Quality of surimi (minced meat of fish) usually decrease due to denaturation of protein. Addition of cryoprotective agent such as reducing sugar as anti denaturation of protein is very important during storage of frozen surimi.
Agustini, T. W. (Tri), Darmanto, Y. S. (Y), Putri, D. P. (Danar)   +2 more
core