Results 181 to 190 of about 18,475 (222)

Cryopreservation of Fish Sperm

2007
This chapter provides detailed descriptions of the protocol used for fish sperm cryopreservation. The principles described can be applied to any species, but details are also given for individual fish species. Successful cryopreservation of fish spermatozoa depends on a range of factors including the collection of high quality sperm, equilibration ...
Eugeny, Kopeika, Julia, Kopeika, Tiantian, Zhang   +2 more
openaire   +2 more sources

Sperm cryopreservation: State of the art

Journal of In Vitro Fertilization and Embryo Transfer, 1989
Despite the lack of adequately controlled clinical trials and sophisticated cryopreservation protocols, infertility experts increasingly employ banked semen for TI, satisfied by the expectation of 10% monthly fecunditied and cumulative pregnancy rates of 65–75%.
D P, Wolf, P E, Patton
openaire   +2 more sources

Liposomes for cryopreservation of bovine sperm

Theriogenology, 2011
In this study, the effect of various unilamellar liposomes on cryopreservation of bovine spermatozoa has been investigated. Liposomes were composed of saturated lipids with various acyl chain lengths: DSPC (18:0), DPPC (16:0), DMPC (14:0), or DLPC (12:0). Alternatively, liposomes were prepared using unsaturated egg phosphatidylcholine (EPC) or DOPC (18:
Röpke, T, Oldenhof, H, Leiding, C, Sieme, H, Bollwein, Heiner, Wolkers, W F   +5 more
openaire   +2 more sources

Cryopreservation of Horse Sperm

Cryopreservation is currently the only strategy for long-term conservation of equine sperm. To get optimal post-thaw sperm survival, carefully following each step of the freezing protocol is crucial. First, one needs to obtain and exhaustively analyze an ejaculate of good sperm quality.
Jordi, Miró, Iris, Martínez-Rodero, Marc, Yeste, Jaime, Catalán   +3 more
openaire   +2 more sources

Bacterial risk and sperm cryopreservation

Andrologia, 2004
Prior to sperm cryopreservation, French guidelines only recommend viral screening for serological status towards human immunodeficiency virus, hepatitis B and C viruses and Treponema palidum. The probability of semen infection by other bacterial pathogens is not taken into consideration by the current recommendations. The objective of the present study
R, Levy, F, Grattard, I, Maubon, A, Ros, B, Pozzetto   +4 more
openaire   +2 more sources

Sperm Cryopreservation in Boars

Cryopreservation is the procedure that allows preserving cells, including gametes, for long time periods while maintaining their functionality, through the use of cryogenic temperature. Currently, sperm cryopreservation is a crucial-assisted reproductive biotechnology in pigs since it is the only effective and efficient method for long-term ...
Inmaculada, Parrilla, María A, Gil, Cristina, Cuello, Emilio A, Martinez   +3 more
openaire   +2 more sources

Rabbit Sperm Cryopreservation

Sperm cryopreservation is an excellent way to store genetic materials for a long period. Semen cryopreservation is associated with structural and functional damage of the spermatozoa caused by cold shock, osmotic stress, intracellular ice crystal formation, and the production of reactive oxygen species, which reduce their functional lifespan and ...
Maria Pilar, Viudes de Castro, Francisco, Marco-Jimenez, Jose Salvador, Vicente   +2 more
openaire   +2 more sources

Cryopreservation of epididymal stallion sperm

Cryobiology, 2014
Any event that makes semen collection or mating impossible, such as death, castration, or injury, may terminate a stallion's breeding career. Fortunately, stallion sperm which are capable of fertilization can be harvested from the epididymis, and frozen for future use.
M, Olaciregui, L, Gil, A, Montón, V, Luño, R A, Jerez, J I, Martí   +5 more
openaire   +2 more sources

Cryopreservation of Plagiognathops microlepis sperm

Cryobiology, 2018
Sperm was collected from cultured male fish and cryopreserved in 0.25 ml straws for the study of sperm cryopreservation. Different parameters were evaluated, including extender, dilution ratio, cryoprotectant type and concentration, equilibrium time, cooling height (in a two-step cooling protocol), and thawing temperature.
Chen, Du, Ying-Li, Han, Ying-Xue, Shi, Jun-Quan, Zhu   +3 more
openaire   +2 more sources

Cryopreservation of epididymal dog sperm

Animal Reproduction Science, 2001
A method of cryopreservation was developed for sperm salvaged from the cauda epididymis and vas deferens of domestic dog testes. Four modifications of the glycerol concentration of a buffer used for cryopreservation of dog ejaculates and two freezing rates were assessed for their effect upon post-thaw spermatozoal motility and morphology.
D A, Hewitt, R, Leahy, I M, Sheldon, G C, England   +3 more
openaire   +4 more sources