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Sperm Cryopreservation Methods
2012There are multiple clinical situations where cryopreservation of sperm is required including sperm banking prior to chemotherapy or radiation treatment for cancer, donor sperm for couples without a male partner, and various etiologies of male factor infertility. Bunge and Sherman presented the first pregnancy resulting from the thawing of cryopreserved
Tiffany, Justice, Greg, Christensen
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Sperm cryopreservation in adolescents with cancer
European Journal of Obstetrics & Gynecology and Reproductive Biology, 2021To evaluate whether sperm banking is under-used in adolescents with cancer.We reviewed our 33 years long experience of semen cryopreservation and identified male subjects with cancer aged 14-21 years who banked their sperm. We hypothesized as a referring model a constant rate of referral in every age category (uniform distribution).
Marco Reschini +9 more
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Zebrafish Sperm Cryopreservation
2004Publisher Summary This chapter reviews the important elements that should be considered when developing a sperm cryopreservation protocol in zebrafish. It also presents an alternative freezing method and discusses further considerations and future directions for the continued optimization of zebrafish cryopreservation. The goal of cryopreservation is
Stéphane, Berghmans +3 more
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Cryopreservation of Pooled Sperm Samples
2021Cryopreservation of sperm cells is currently the most efficient tool for managing large and small collections of valuable genetic resources. Cryopreservation minimizes expenses for animal and facility maintenance such as personnel, water, power, and space.
Jennifer L, Matthews, Zoltan M, Varga
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Cryopreservation of Fish Sperm
2007This chapter provides detailed descriptions of the protocol used for fish sperm cryopreservation. The principles described can be applied to any species, but details are also given for individual fish species. Successful cryopreservation of fish spermatozoa depends on a range of factors including the collection of high quality sperm, equilibration ...
Eugeny, Kopeika +2 more
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Sperm cryopreservation: State of the art
Journal of In Vitro Fertilization and Embryo Transfer, 1989Despite the lack of adequately controlled clinical trials and sophisticated cryopreservation protocols, infertility experts increasingly employ banked semen for TI, satisfied by the expectation of 10% monthly fecunditied and cumulative pregnancy rates of 65–75%.
D P, Wolf, P E, Patton
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Liposomes for cryopreservation of bovine sperm
Theriogenology, 2011In this study, the effect of various unilamellar liposomes on cryopreservation of bovine spermatozoa has been investigated. Liposomes were composed of saturated lipids with various acyl chain lengths: DSPC (18:0), DPPC (16:0), DMPC (14:0), or DLPC (12:0). Alternatively, liposomes were prepared using unsaturated egg phosphatidylcholine (EPC) or DOPC (18:
Röpke, T +5 more
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Cryopreservation of Horse Sperm
Cryopreservation is currently the only strategy for long-term conservation of equine sperm. To get optimal post-thaw sperm survival, carefully following each step of the freezing protocol is crucial. First, one needs to obtain and exhaustively analyze an ejaculate of good sperm quality.Jordi, Miró +3 more
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Bacterial risk and sperm cryopreservation
Andrologia, 2004Prior to sperm cryopreservation, French guidelines only recommend viral screening for serological status towards human immunodeficiency virus, hepatitis B and C viruses and Treponema palidum. The probability of semen infection by other bacterial pathogens is not taken into consideration by the current recommendations. The objective of the present study
R, Levy +4 more
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Sperm Cryopreservation in Boars
Cryopreservation is the procedure that allows preserving cells, including gametes, for long time periods while maintaining their functionality, through the use of cryogenic temperature. Currently, sperm cryopreservation is a crucial-assisted reproductive biotechnology in pigs since it is the only effective and efficient method for long-term ...Inmaculada, Parrilla +3 more
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