Results 81 to 90 of about 31,024 (234)

Diagnostic yield of OCT3/4 reflex testing in testicular biopsy

Histopathology, EarlyView.
GCNIS detection frequency was 48/3353 (1.4%) testicular biopsies from 42/1870 (2.2%) men who underwent testicular biopsy with or without testicular sperm extraction (TESE). OCT3/4 was particularly helpful for detecting interstitial seminoma, GCNIS coexisting with mature spermatogenesis, and focal GCNIS.
Roselyne Choiniere, Willem PA Boellaard, Geert JLH van Leenders   +2 more
wiley   +1 more source

Quantitative proteomic characterization of human sperm cryopreservation: using data-independent acquisition mass spectrometry

BMC Urology, 2019
Background Human sperm cryopreservation is a simple and effective approach for male fertility preservation. Methods To identify potential proteomic changes in this process, data-independent acquisition (DIA), a technology with high quantitative accuracy ...
Longlong Fu, Qi An, Kaishu Zhang, Ying Liu, Yue Tong, Jianfeng Xu, Fang Zhou, Xiaowei Wang, Ying Guo, Wenhong Lu, Xiaowei Liang, Yiqun Gu   +11 more
doaj   +1 more source

Targeting Cellular Lipid Rafts for Dynamic Nuclear Polarization Nuclear Magnetic Resonance

ChemBioChem, Volume 27, Issue 5, 13 March 2026.
Illuminating lipid rafts within intact cells using dynamic nuclear polarization (DNP). Targeting lipid rafts with the protein ostreolysin A allows the selective delivery of DNP‐compatible radicals, generating hyperpolarization of nuclear spins in the lipid raft. Lipid rafts serve as important platforms for membrane and signaling proteins.
Sarah A. Overall, Agnes Eck, Ancy T. Wilson, Dorothea Pinotsi, Sina J. Hartmann, Katja Packebusch, Snorri Th. Sigurdsson, Alexander B. Barnes   +7 more
wiley   +1 more source

Low-density Lipoprotein Improves Motility and Plasma Membrane Integrity of Cryopreserved Canine Epididymal Spermatozoa [PDF]

Asian-Australasian Journal of Animal Sciences, 2016
Cryopreservation of caudal epididymal spermatozoa is an effective technique to conserve genetic potentials of superior dogs when it is not possible to collect ejaculated spermatozoa. Although hen egg yolk is commonly supplemented into the semen extender,
N. Prapaiwan, T. Tharasanit, S. Punjachaipornpol, D. Yamtang, A. Roongsitthichai, W. Moonarmart, K. Kaeoket, S. Manee-in   +7 more
doaj   +1 more source

Bottom‐Up Ice Growth Geometry Attenuates Shear Stress and Improves the Cryopreservation of Hematopoietic Stem/Progenitor Cells Under Low DMSO Concentrations

Biotechnology and Bioengineering, Volume 123, Issue 3, Page 582-597, March 2026.
ABSTRACT Studies on cell cryopreservation have been limited by the complexity of the freezing process and challenges on controlling ice formation, managing cooling rates, and optimizing cryoprotectant concentrations. The objective of this study is to evaluate the impact of bottom‐up and conventional radial freezing on the viability of mammalian cells ...
Rafaela Ouro Neves, Pedro Sena Rego, Marta H. G. Costa, Isabel Bogalho, Andreia Duarte, Claúdia L. da Silva, Frederico Castelo Ferreira, Vitor Geraldes, Miguel A. Rodrigues   +8 more
wiley   +1 more source

Cryopreservation enhances vacuolization in human spermatozoa

Asian Pacific Journal of Reproduction, 2017
Objective: To evaluate the impact of freezing–thawing on the human sperm head vacuoles and the potential value of motile sperm organelle morphology examination for selection of frozen-thaw spermatozoa.Methods: In 30 sperm samples from infertile men ...
Nahid Yari, Sahabeh Etebary, Mohammad Ali Khalili, Ali Reza Talebi   +3 more
doaj   +1 more source

Protective effect and mechanisms of marine antifreeze protein product on sperm cryodamage in livestock

浙江大学学报. 农业与生命科学版, 2020
Antifreeze proteins (AFPs), a class of antifreeze substances, were initially found to bind to ice crystals in vivo, thereby contributing to inhibition of the growth of ice crystals that enabled polar fish to survive at the temperatures below freezing ...
HENG Nuo, GUO Yong, CHEN Yu, WANG Liang, SHENG Xihui, WANG Xiangguo, XING Kai, NI Hemin, QI Xiaolong   +8 more
doaj   +1 more source

Fertility preservation in women with sarcoma: A real‐world experience

International Journal of Cancer, Volume 158, Issue 5, Page 1178-1183, 1 March 2026.
What's New? Sarcomas frequently affect adolescent girls and young women, but limited data exist on ovarian function following sarcoma treatment. This observational cohort study evaluated the feasibility, timing, and clinical relevance of fertility preservation in young women treated for high‐grade sarcomas or desmoid tumors.
Guillaume Beinse, Pascaline Boudou‐Rouquette, Sixtine de Percin, Anne Fortin, Johanna Noel, Sarah Kreps, Jérémy Sroussi, Virginie Barraud‐Lange, Jérôme Alexandre, François Goldwasser, Camille Tlemsani, Catherine Poirot   +11 more
wiley   +1 more source

Post‐Thawing Viability of the Microalgae Scenedesmus dimorphus: The Importance of Species‐Specific Cryopreservation

New Zealand Journal of Marine and Freshwater Research, Volume 60, Issue 1, March 2026.
Cryopreservation of microalgae is crucial for preserving strains that are valuable for biotechnology and industry. The cryopreservation of Scenedesmus dimorphus is particularly relevant due to its emerging role in sustainable biotechnology, including its high lipid accumulation for biofuel production, adaptability to variable lighting conditions, and ...
Martha Prieto‐Guevara, César Jiménez‐Velásquez, Yamid Hernández‐Julio, José Espinosa‐Araujo, Víctor Atencio‐García   +4 more
wiley   +1 more source

Cryopreservation of epididymal dog sperm

Animal Reproduction Science, 2001
A method of cryopreservation was developed for sperm salvaged from the cauda epididymis and vas deferens of domestic dog testes. Four modifications of the glycerol concentration of a buffer used for cryopreservation of dog ejaculates and two freezing rates were assessed for their effect upon post-thaw spermatozoal motility and morphology.
D A, Hewitt, R, Leahy, I M, Sheldon, G C, England   +3 more
openaire   +4 more sources