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Microfluidics for Sperm Selection
2014Traditional methods for sperm selection such as swim-up and isopycnic (density gradient) centrifugation involve prolonged periods of incubation and/or serial centrifugations. Prolonged incubation of sperm with other cellular components of semen and serial centrifugation expose motile sperm to reactive oxygen species.
Gary D. Smith +2 more
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Sex selection by sperm separation and insemination
Fertility and Sterility, 1984The Ericsson albumin filtration technique was used to collect a fraction rich in Y sperm for selective insemination in couples desiring a male infant. Of 35 conceptions in which sex was known at delivery or spontaneous abortion, there were 28 males (80%).
S L, Corson +4 more
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On the selective elimination of Y-bearing sperm
Immunogenetics, 1978The potential use of antibodies that selectively recognize either X-bearing or Y-bearing sperm is self-evident. Thus our attention was directed to the fact that under optimal conditions, H-Y antibody lyses 50% of mouse spermatozoa. Accordingly, we asked whether expression of H-Y antigen is haploid in spermatozoa from XY male mice heterozygous for the ...
Ohno, S, Wachtel, S S
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Sperm Selection Capacity of Cervical Mucus
Archives of Andrology, 1984Seventeen couples who attended the Infertility Clinic for routine fertility investigations were used in the study. Complete morphological examinations were performed on the ejaculates and cervical mucus samples following coitus in order to determine the morphological selective capacity of the mucus.
E, Pretorius +3 more
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Sperm Selection Based on Electrostatic Charge
2012Charge is a fundamental property of all forms of matter that exhibit attraction or repulsion in the presence of another charged particle. This electrokinetic property occurs when the particles exhibiting a net negative or positive charge are subjected to an external electric field that exerts an electrostatic force between them. Sperm surface membranes
Luke, Simon +2 more
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XY Sperm Separation for Sex Selection
Urologic Clinics of North America, 1987At present, laboratory methods that can successfully differentiate X- from Y-bearing sperm do exist; namely, staining with quinacrine mustard and DNA flow cytometry. However, methods that can successfully and reproducibly separate X- from Y-bearing sperm with resultant functionally intact sperm have yet to be developed.
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Sperm Selection by Colloid Centrifugation
Colloid centrifugation has several uses within spermatology. It has traditionally been used as a biomimetic method to select the most robust, fertile spermatozoa from a sperm sample. However, any sub-population can be selected, or the spermatozoa can be separated from seminal plasma, by changing the density of the colloid used.openaire +2 more sources
Intracytoplasmic Morphology-Selected Sperm Injection
2012Approximately 40% of sterility in couples can be attributed to male subfertility and intracytoplasmic sperm injection (ICSI) has become a powerful tool in assisted reproduction to overcome male infertility. Intracytoplasmic morphologically selected sperm injection (IMSI) is an advanced and sophisticated method of ICSI, where prior to sperm injection ...
Luke, Simon +2 more
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[Sperm quality and selection].
Journal de gynecologie, obstetrique et biologie de la reproduction, 2008Selection of a live and morphologically normal spermatozoa is a fundamental stage in ICSI success because of its potential effects on early and late embryo development. In addition to the routine tests such as the spermogram and the spermocytogram, a number of tests have been developed for this purpose : the hyaluronic acid test, which measures the ...
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