Results 41 to 50 of about 130,477 (208)

Lipid Nanoparticles for the Delivery of CRISPR/Cas9 Machinery to Enable Site‐Specific Integration of CFTR and Mutation‐Agnostic Disease Rescue

Advanced Functional Materials, EarlyView.
Lipid nanoparticles (LNPs) are optimized to co‐deliver Cas9‐encoding messenger RNA (mRNA), a single guide RNA (sgRNA) targeting the endogenous cystic fibrosis transmembrane conductance regulator (CFTR) gene, and homologous linear double‐stranded donor DNA (ldsDNA) templates encoding CFTR.
Ruth A. Foley, Paul G. Ayoub, Vrishti Sinha, Colin Juett, Alicia Sanoyca, Emily C. Duggan, Lindsay E. Lathrop, Priyanka Bhatt, Kevin Coote, Beate Illek, Brigitte N. Gomperts, Donald B. Kohn, Steven J. Jonas   +12 more
wiley   +1 more source

Development and application of EpitopeScan, a Python3 toolset for mutation tracking in SARS-CoV-2 immunogenic epitopes

Frontiers in Immunology
IntroductionOutbreaks of coronaviruses and especially the recent COVID-19 pandemic emphasize the importance of immunological research in this area to mitigate the effect of future incidents.
Alexander Kovalenko, Sebastien Viatte, Sebastien Viatte, Sebastien Viatte   +3 more
doaj   +1 more source

Towards building hybrid biological/in silico neural networks for motor neuroprosthetic control

Frontiers in Neurorobotics, 2015
In this article, we introduce the Bioinspired Neuroprosthetic Design Environment (BNDE) as a practical platform for the development of novel brain machine interface (BMI) controllers which are based on spiking model neurons.
Mehmet eKocaturk, Mehmet eKocaturk, Halil Ozcan Gulcur, Resit eCanbeyli   +3 more
doaj   +1 more source

Enhancing Direct Solar Water Splitting via ALD of Multifunctional TiO2/Pt Nanoparticle Coatings With Engineered Interfaces to GaAs/GaInP Tandem Cells

Advanced Functional Materials, EarlyView.
Multifunctional atomic layer deposited coatings and interface treatments enhance direct solar water splitting on GaAs/GaInP tandem cells. Optimized TiO2/Pt nanoparticle bilayers ensure durability and catalytic efficiency with minimal optical losses, while H2 plasma pretreatments maximize photovoltage and interfacial charge extraction.
Tim F. Rieth, Verena Streibel, Oliver Bienek, Sergej Levashov, Johannes Dittloff, Kristof Möller, Johanna Eichhorn, Matthias M. May, Ian D. Sharp   +8 more
wiley   +1 more source

Next‐Generation Bio‐Reducible Lipids Enable Enhanced Vaccine Efficacy in Malaria and Primate Models

Advanced Functional Materials, EarlyView.
Structure–activity relationship (SAR) optimization of bio‐reducible ionizable lipids enables the development of highly effective lipid nanoparticle (LNP) mRNA vaccines. Lead LNPs show superior tolerability and antibody responses in rodents and primates, outperforming approved COVID‐19 vaccine lipids.
Ruben De Coen, Sabah Kasmi, Shrinivas Dumbre, Lies Baekens, Elise Seynaeve, Louise Van den Hauwe, Niccolò Cantini, Aello Garantoudi, Filipe Branco Madeira, Alain Marrot, Emma Tuypens, Sofie Seghers, Anna Kuchmiy, Elisabeth Brabants, Bart Vanderborght, Ine Maes, Sarah Vandenberghe, Jessica Filtjens, Alícia Gordún Peiró, Daliya Kancheva, Ayla Debraekeleer, Florence Lambolez, Emily De Lombaerde, Yong Chen, Rafael Miyazawa Martins, Pauline Formaglio, Damya Laoui, Sofie Deschoemaeker, Bruno G. De Geest, Rogerio Amino, Stefaan De Koker   +30 more
wiley   +1 more source

A spike is a spike: On the universality of spike features in four epilepsy models

Epilepsia Open
Objective Frequency properties of the EEG characteristics of different seizure types including absence seizures have been described for various rodent models of epilepsy.
Armen Sargsyan, Pablo M. Casillas‐Espinosa, Dmitri Melkonian, Terence J. O'Brien, Gilles vanLuijtelaar   +4 more
doaj   +1 more source

Neuromorphic Readout for Hadron Calorimeters

Particles
We simulate hadrons impinging on a homogeneous lead tungstate (PbWO4) calorimeter using GEANT4 software to investigate how the resulting light yield and its temporal structure, as detected by an array of light-sensitive sensors, can be processed by a ...
Enrico Lupi, Abhishek, Max Aehle, Muhammad Awais, Alessandro Breccia, Riccardo Carroccio, Long Chen, Abhijit Das, Andrea De Vita, Tommaso Dorigo, Nicolas Ralph Gauger, Ralf Keidel, Jan Kieseler, Anders Mikkelsen, Federico Nardi, Xuan Tung Nguyen, Fredrik Sandin, Kylian Schmidt, Pietro Vischia, Joseph Willmore   +19 more
doaj   +1 more source

CRISPR/Cas9‐Assisted Microrobots for Fast and Ultrasensitive “On‐The‐Fly” Next‐Generation DNA Detection

Advanced Functional Materials, EarlyView.
This work presents self‐propelled CRISPR/Cas9‐functionalized Au–MRs for rapid, amplification‐free, “on‐the‐fly” DNA detection. By harnessing motion‐assisted signal recovery, the platform achieved the limit of detection in low fM DNA concentrations, enabling detection across a wide dynamic range within only 5 min, which is significantly faster than any ...
Jyoti, Petr Michálek, Zbyněk Heger, Martin Pumera   +3 more
wiley   +1 more source

Transparent Inorganic–Organic Bilayer Neural Electrode Array and Integration to Miniscope System for In Vivo Calcium Imaging and Electrophysiology

Advanced Functional Materials, EarlyView.
This study presents the BioCLEAR system, a highly transparent and conductive neural electrode array composed of silver nanowires (AgNWs) and doped PEDOT:PSS, enabling neural recordings with minimal optical artifacts. When integrated with a GRIN lens, this cost‐effective neural implant allows simultaneous electrophysiological recording and GCaMP6‐based ...
Dongjun Han, Kevin Cheng, Swathy Sampath Kumar, Anil Bollimunta, Elizabeth E. Torr, Juhwan Kim, Kyoungmin Kim, Hyungjun Choi, Seongkwon Hwang, Sarah Brodnick, David Cheng, Lindsay Gray, Yei Hwan Jung, William L. Murphy, Aaron J. Suminski, Jonathan J. Nassi, Justin C. Williams, Dong‐Wook Park   +17 more
wiley   +1 more source

Empirical methods for controlling false positives and estimating confidence in ChIP-Seq peaks

BMC Bioinformatics, 2008
Background High throughput signature sequencing holds many promises, one of which is the ready identification of in vivo transcription factor binding sites, histone modifications, changes in chromatin structure and patterns of DNA methylation across ...
Courdy Samir J, Nix David A, Boucher Kenneth M   +2 more
doaj   +1 more source