Results 81 to 90 of about 12,963 (292)
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley +1 more source
Rice Plant Development: from Zygote to Spikelet [PDF]
Rice is becoming a model plant in monocotyledons and a model cereal crop. For better understanding of the rice plant, it is essential to elucidate the developmental programs of the life cycle. To date, several attempts have been made in rice to categorize the developmental processes of some organs into substages.
Jun-Ichi, Itoh +7 more
openaire +2 more sources
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Technical Notes: The grass spikelet formula: an aid in teaching and identification [PDF]
The structure and arrangement of the grass spikelet may be summarized by use of a spikelet formula. The parts of the formula are stacked vertically to correspond to the parts of the grass spikelet.
Columbus, J. T., Allred, K. W.
core +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Histological analyses of spikelet hulls at maturity. [PDF]
(A). Spikelet hulls of the WT control and hgw mutant. Dotted lines indicate positions of cross-sections. (B). Scanning electron micrographs showing cross-sections of the WT control and hgw mutant. The yellow line outlines the overall circumference of the
Yonghong Zhang (20918) +6 more
core +1 more source
Despite its very recent history of cultivation in Ethiopia, rice is one of the potential grain crops that could contribute to the efforts for the realization of food security in the country.
Heluf Gebrekidan, Mulugeta Seyoum
doaj
With global warming, rice plants may be subjected to heat stress more regularly during the heat-sensitive flowering stage, causing spikelet sterility and grain yield loss.
Chao Wu +6 more
doaj +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source

