gem‐Diethyl Pyrroline Nitroxide Spin Labels: Synthesis, EPR Characterization, Rotamer Libraries and Biocompatibility [PDF]
ChemistryOpen, 2019 The availability of bioresistant spin labels is crucial for the optimization of site‐directed spin labeling protocols for EPR structural studies of biomolecules in a cellular context.
Dr. Stephanie Bleicken +9 more
doaj +6 more sources
A new perspective on membrane-embedded Bax oligomers using DEER and bioresistant orthogonal spin labels [PDF]
Scientific Reports, 2019 Bax is a Bcl-2 protein crucial for apoptosis initiation and execution, whose active conformation is only partially understood. Dipolar EPR spectroscopy has proven to be a valuable tool to determine coarse-grained models of membrane-embedded Bcl-2 ...
Markus Teucher +7 more
doaj +3 more sources
New Developments in Spin Labels for Pulsed Dipolar EPR [PDF]
Molecules, 2014 Spin labelling is a chemical technique that enables the integration of a molecule containing an unpaired electron into another framework for study. Given the need to understand the structure, dynamics, and conformational changes of biomacromolecules ...
Alistair J. Fielding +3 more
doaj +3 more sources
Spin-Lattice Relaxation Rates of Lipid Spin Labels as a Measure of Their Rotational Diffusion Rates in Lipid Bilayer Membranes [PDF]
Membranes, 2022 The spin-lattice relaxation rate (T1−1) of lipid spin labels obtained from saturation recovery EPR measurements in deoxygenated membranes depends primarily on the rate of the rotational diffusion of the nitroxide moiety within the lipid bilayer.
Witold K. Subczynski, Justyna Widomska
doaj +2 more sources
Probing the solution structure of the E. coli multidrug transporter MdfA using DEER distance measurements with nitroxide and Gd(III) spin labels. [PDF]
Sci Rep, 2019 Methodological and technological advances in EPR spectroscopy have enabled novel insight into the structural and dynamic aspects of integral membrane proteins.
Yardeni EH +10 more
europepmc +2 more sources
Pseudocontact shifts from mobile spin labels [PDF]
Physical Chemistry, Chemical Physics - PCCP, 2016 This paper presents a detailed analysis of the pseudocontact shift (PCS) field induced by a mobile spin label that is viewed as a probability density distribution with an associated effective magnetic susceptibility anisotropy.
Kuprov, Ilya, Suturina, Elizaveta
core +4 more sources
A Simple Method of Synthesis of 3-Carboxy-2,2,5,5-Tetraethylpyrrolidine-1-oxyl and Preparation of Reduction-Resistant Spin Labels and Probes of Pyrrolidine Series [PDF]
Molecules, 2021 Stable free radicals are widely used as molecular probes and labels in various biophysical and biomedical research applications of magnetic resonance spectroscopy and imaging.
Sergey A. Dobrynin +11 more
doaj +2 more sources
A Comparison of Cysteine-Conjugated Nitroxide Spin Labels for Pulse Dipolar EPR Spectroscopy [PDF]
Molecules, 2021 The structure-function and materials paradigms drive research on the understanding of structures and structural heterogeneity of molecules and solids from materials science to structural biology.
Katrin Ackermann +2 more
doaj +2 more sources
Simulating the distance distribution between spin-labels attached to proteins. [PDF]
J Phys Chem B, 2015 EPR/DEER spectroscopy is playing an increasingly important role in the characterization of the conformational states of proteins. In this study, force field parameters for the bifunctional spin-label (RX) used in EPR/DEER are parametrized and tested with
Islam SM, Roux B.
europepmc +2 more sources
Flexibilities of isoindoline-derived spin labels for nucleic acids by orientation selective PELDOR.
Physical Chemistry, Chemical Physics - PCCP, 2016 Pulsed electron electron double resonance experiments with rigid spin labels can reveal very detailed information about the structure and conformational flexibility of nucleic acid molecules.
N. Erlenbach +5 more
semanticscholar +3 more sources