Spin-Lattice Relaxation Rates of Lipid Spin Labels as a Measure of Their Rotational Diffusion Rates in Lipid Bilayer Membranes [PDF]
Membranes, 2022 The spin-lattice relaxation rate (T1−1) of lipid spin labels obtained from saturation recovery EPR measurements in deoxygenated membranes depends primarily on the rate of the rotational diffusion of the nitroxide moiety within the lipid bilayer.
Witold K. Subczynski, Justyna Widomska
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gem‐Diethyl Pyrroline Nitroxide Spin Labels: Synthesis, EPR Characterization, Rotamer Libraries and Biocompatibility [PDF]
ChemistryOpen, 2019 The availability of bioresistant spin labels is crucial for the optimization of site‐directed spin labeling protocols for EPR structural studies of biomolecules in a cellular context.
Dr. Stephanie Bleicken +9 more
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New Developments in Spin Labels for Pulsed Dipolar EPR [PDF]
Molecules, 2014 Spin labelling is a chemical technique that enables the integration of a molecule containing an unpaired electron into another framework for study. Given the need to understand the structure, dynamics, and conformational changes of biomacromolecules ...
Alistair J. Fielding +3 more
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A Simple Method of Synthesis of 3-Carboxy-2,2,5,5-Tetraethylpyrrolidine-1-oxyl and Preparation of Reduction-Resistant Spin Labels and Probes of Pyrrolidine Series [PDF]
Molecules, 2021 Stable free radicals are widely used as molecular probes and labels in various biophysical and biomedical research applications of magnetic resonance spectroscopy and imaging.
Sergey A. Dobrynin +11 more
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A Comparison of Cysteine-Conjugated Nitroxide Spin Labels for Pulse Dipolar EPR Spectroscopy [PDF]
Molecules, 2021 The structure-function and materials paradigms drive research on the understanding of structures and structural heterogeneity of molecules and solids from materials science to structural biology.
Katrin Ackermann +2 more
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A new perspective on membrane-embedded Bax oligomers using DEER and bioresistant orthogonal spin labels [PDF]
Scientific Reports, 2019 Bax is a Bcl-2 protein crucial for apoptosis initiation and execution, whose active conformation is only partially understood. Dipolar EPR spectroscopy has proven to be a valuable tool to determine coarse-grained models of membrane-embedded Bcl-2 ...
Markus Teucher +7 more
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Membrane Depth Measurements of E Protein by 2H ESEEM Spectroscopy in Lipid Bilayers [PDF]
BiophysicaA topological analysis was performed by taking ESEEM measurements of site-specifically labeled E protein from SARS-CoV-2. The intensity of deuterium modulation arising from either deuterated solvent or deuterated lipid acyl chains revealed exposure to ...
Andrew K. Morris +2 more
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Structural dynamics of calmodulin-ryanodine receptor interactions: electron paramagnetic resonance using stereospecific spin labels [PDF]
Scientific Reports, 2018 We have used electron paramagnetic resonance, with rigid and stereospecific spin labels, to resolve structural states in calmodulin (CaM), as affected by binding of Ca and a CaM-binding peptide (RyRp) derived from the ryanodine receptor (RyR), the Ca ...
Cheng Her +3 more
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Strategies to identify and suppress crosstalk signals in double electron–electron resonance (DEER) experiments with gadoliniumIII and nitroxide spin-labeled compounds [PDF]
Magnetic Resonance, 2020 Double electron–electron resonance (DEER) spectroscopy applied to orthogonally spin-labeled biomolecular complexes simplifies the assignment of intra- and intermolecular distances, thereby increasing the information content per sample.
M. Teucher +5 more
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Site-specific structural order in Alzheimer's Aβ42 fibrils [PDF]
Royal Society Open Science, 2018 Deposition of amyloid fibrils is a pathological hallmark of Alzheimer's disease. Aβ42 is the major protein whose aggregation leads to the formation of these fibrils.
Hongsu Wang +3 more
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