Studies of transmembrane peptides by pulse dipolar spectroscopy with semi-rigid TOPP spin labels. [PDF]
Eur Biophys J, 2021 Electron paramagnetic resonance (EPR)-based pulsed dipolar spectroscopy measures the dipolar interaction between paramagnetic centers that are separated by distances in the range of about 1.5–10 nm.
Tkach I, Diederichsen U, Bennati M.
europepmc +2 more sources
Strategies to identify and suppress crosstalk signals in double electron–electron resonance (DEER) experiments with gadoliniumIII and nitroxide spin-labeled compounds [PDF]
Magnetic Resonance, 2020 Double electron–electron resonance (DEER) spectroscopy applied to orthogonally spin-labeled biomolecular complexes simplifies the assignment of intra- and intermolecular distances, thereby increasing the information content per sample.
M. Teucher +5 more
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Light-induced pulsed dipolar spectroscopy and other electron spin resonance studies of photogenerated paramagnetic species [PDF]
, 2023 This thesis is concerned with the development of new methodologies in the field of Light-induced Pulsed Electron Spin Resonance Dipolar Spectroscopy (LiPDS), with the aim of applying them to the structural investigation of complex biological ...
Bertran, Arnau
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Site-specific structural order in Alzheimer's Aβ42 fibrils [PDF]
Royal Society Open Science, 2018 Deposition of amyloid fibrils is a pathological hallmark of Alzheimer's disease. Aβ42 is the major protein whose aggregation leads to the formation of these fibrils.
Hongsu Wang +3 more
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Analytical Biochemistry, 2008 Lipopolysaccharide (LPS), a major component of the outer membranes of gram-negative bacteria, is composed of a polysaccharide chain attached to a lipid A base that contains a disaccharide headgroup with two negative phosphate groups and at least four acyl chains.
Lynn E, Bretscher +2 more
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RosettaEPR: rotamer library for spin label structure and dynamics. [PDF]
PLoS ONE, 2013 An increasingly used parameter in structural biology is the measurement of distances between spin labels bound to a protein. One limitation to these measurements is the unknown position of the spin label relative to the protein backbone. To overcome this
Nathan S Alexander +5 more
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chiLife: An open-source Python package for in silico spin labeling and integrative protein modeling.
PLoS Computational Biology, 2023 Here we introduce chiLife, a Python package for site-directed spin label (SDSL) modeling for electron paramagnetic resonance (EPR) spectroscopy, in particular double electron-electron resonance (DEER).
Maxx H Tessmer, Stefan Stoll
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Spin-labeled polyribonucleotides [PDF]
Nucleic Acids Research, 1980 Poly (U), poly (C) and poly (A) were spin labeled with N-(2,2,5,5-tetramethyl-3-carbonylpyrroline-1-oxyl)-imidazole. This spin label interacts selectively with 2' OH ribose groups of polynucleotides and does not modify the nucleic acid bases. The extent of spin labeling is not dependent upon the nature of the base and is entirely determined by rigidity
A I, Petrov, B I, Sukhorukov
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Molecular Oxygen as a Probe Molecule in EPR Spin Labeling Studies of Membrane Structure and Dynamics
Oxygen, 2022 Molecular oxygen (O2) is the perfect probe molecule for membrane studies carried out using the saturation recovery EPR technique. O2 is a small, paramagnetic, hydrophobic enough molecule that easily partitions into a membrane’s different phases and ...
Witold K. Subczynski +3 more
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Structural Impact of Selected Retinoids on Model Photoreceptor Membranes
Membranes, 2023 Photoreceptor membranes have a unique lipid composition. They contain a high level of polyunsaturated fatty acids including the most unsaturated fatty acid in nature, docosahexaenoic acid (22:6), and are enriched in phosphatidylethanolamines.
Szymon Radzin +8 more
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