gem‐Diethyl Pyrroline Nitroxide Spin Labels: Synthesis, EPR Characterization, Rotamer Libraries and Biocompatibility [PDF]
ChemistryOpen, 2019 The availability of bioresistant spin labels is crucial for the optimization of site‐directed spin labeling protocols for EPR structural studies of biomolecules in a cellular context.
Dr. Stephanie Bleicken +9 more
doaj +5 more sources
Spin-Lattice Relaxation Rates of Lipid Spin Labels as a Measure of Their Rotational Diffusion Rates in Lipid Bilayer Membranes [PDF]
Membranes, 2022 The spin-lattice relaxation rate (T1−1) of lipid spin labels obtained from saturation recovery EPR measurements in deoxygenated membranes depends primarily on the rate of the rotational diffusion of the nitroxide moiety within the lipid bilayer.
Witold K. Subczynski, Justyna Widomska
doaj +4 more sources
New Developments in Spin Labels for Pulsed Dipolar EPR [PDF]
Molecules, 2014 Spin labelling is a chemical technique that enables the integration of a molecule containing an unpaired electron into another framework for study. Given the need to understand the structure, dynamics, and conformational changes of biomacromolecules ...
Alistair J. Fielding +3 more
doaj +4 more sources
A Simple Method of Synthesis of 3-Carboxy-2,2,5,5-Tetraethylpyrrolidine-1-oxyl and Preparation of Reduction-Resistant Spin Labels and Probes of Pyrrolidine Series [PDF]
Molecules, 2021 Stable free radicals are widely used as molecular probes and labels in various biophysical and biomedical research applications of magnetic resonance spectroscopy and imaging.
Sergey A. Dobrynin +11 more
doaj +2 more sources
A Comparison of Cysteine-Conjugated Nitroxide Spin Labels for Pulse Dipolar EPR Spectroscopy [PDF]
Molecules, 2021 The structure-function and materials paradigms drive research on the understanding of structures and structural heterogeneity of molecules and solids from materials science to structural biology.
Katrin Ackermann +2 more
doaj +2 more sources
A new perspective on membrane-embedded Bax oligomers using DEER and bioresistant orthogonal spin labels [PDF]
Scientific Reports, 2019 Bax is a Bcl-2 protein crucial for apoptosis initiation and execution, whose active conformation is only partially understood. Dipolar EPR spectroscopy has proven to be a valuable tool to determine coarse-grained models of membrane-embedded Bcl-2 ...
Markus Teucher +7 more
doaj +2 more sources
Structural dynamics of calmodulin-ryanodine receptor interactions: electron paramagnetic resonance using stereospecific spin labels [PDF]
Scientific Reports, 2018 We have used electron paramagnetic resonance, with rigid and stereospecific spin labels, to resolve structural states in calmodulin (CaM), as affected by binding of Ca and a CaM-binding peptide (RyRp) derived from the ryanodine receptor (RyR), the Ca ...
Cheng Her +3 more
doaj +2 more sources
Curcumin–Lipid Interactions in PEGylated vs. Conventional Liposomes: A Combined Fluorescence and EPR Study [PDF]
MembranesCurcumin, a natural polyphenol derived from Curcuma longa, is widely recognized for its therapeutic properties. However, its clinical utility is limited because of poor solubility, rapid degradation and hence low bioavailability. To overcome these issues,
Namra Fatima +2 more
doaj +2 more sources
Membrane Depth Measurements of E Protein by 2H ESEEM Spectroscopy in Lipid Bilayers [PDF]
BiophysicaA topological analysis was performed by taking ESEEM measurements of site-specifically labeled E protein from SARS-CoV-2. The intensity of deuterium modulation arising from either deuterated solvent or deuterated lipid acyl chains revealed exposure to ...
Andrew K. Morris +2 more
doaj +2 more sources
Spin Labeling of Surface Cysteines Using a Bromoacrylaldehyde Spin Label [PDF]
Applied Magnetic Resonance, 2021 AbstractStructural investigations of proteins and their biological complexes are now frequently complemented by distance constraints between spin labeled cysteines generated using double electron–electron resonance (DEER) spectroscopy, via site directed spin labeling (SDSL).
Graham Heaven +3 more
openaire +3 more sources