Results 171 to 180 of about 28,256 (215)
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Quarterly Reviews of Biophysics, 1981
The spin label method developed by McConnell 15 years ago is now widely used in studies of the structure and dynamic properties of a variety of the biological systems such as proteins and protein complexes, lipids and membranes, nucleic acids, nucleoproteins, etc.The ESR spectrum of the nitroxide radcal – the spin label – is very sensitive to its ...
S G, Kamzolova, G B, Postnikova
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The spin label method developed by McConnell 15 years ago is now widely used in studies of the structure and dynamic properties of a variety of the biological systems such as proteins and protein complexes, lipids and membranes, nucleic acids, nucleoproteins, etc.The ESR spectrum of the nitroxide radcal – the spin label – is very sensitive to its ...
S G, Kamzolova, G B, Postnikova
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Biochemical and Biophysical Research Communications, 1973
The compound 2,2,6,6-tetramethyl-4-[β-N-ethyleneiminopropionyl] oxypiperidine-I-oxyl is used as a spin-label for RNA. The reaction, effected under rather mild conditions, results in 50–70 nucleotides per spin-label. The temperature dependence of the ESR spectra of spin-labeled RNA is used to estimate temperatures corresponding to the beginning of ...
S, Zavriev +3 more
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The compound 2,2,6,6-tetramethyl-4-[β-N-ethyleneiminopropionyl] oxypiperidine-I-oxyl is used as a spin-label for RNA. The reaction, effected under rather mild conditions, results in 50–70 nucleotides per spin-label. The temperature dependence of the ESR spectra of spin-labeled RNA is used to estimate temperatures corresponding to the beginning of ...
S, Zavriev +3 more
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Electron Spin Resonance: Spin Labels
1981In general biological membranes possess no intrinsic paramagnetism and hence in the unlabeled state do not give rise to an electron spin resonance (ESR) spectrum. The introduction of a stable free radical (“spin label”) thus enables one to use ESR spectroscopy to study specific environments within the membrane.
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Spin-labelled Au nanoparticles
Faraday Discussions, 2004A series of Au nanoparticles functionalised with nitroxide spin labels has been prepared and studied by EPR spectroscopy. Samples with low coverage of the spin label were used to investigate the dynamics of the surface-attached labels at different distances from the Au surface. The rotational correlation times of spin labels vary from 10(-10) s to more
Victor, Chechik +6 more
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Spin Labeling of Potassium Channels
2015Potassium channels are the ion channels most extensively studied by structural techniques. Whereas high-resolution crystal structures have provided key insights into the molecular architecture of these channels, spin labeling studies have helped to unveil the dynamic structural aspects underlying their function.
Dylan, Burdette, Adrian, Gross
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Thrombosis Research, 1976
Abstract Human platelets were labelled with a variety of lipophilic and thiol reagent spin probes. Aggregation was not affected by the lipid probes but was blocked by the covalent protein-directed probes. ESR spectra before and after thrombin treatment indicated that lipid structure of the platelet membrane was not affected to any measurable extent ...
F, Sachs, R D, Feinman
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Abstract Human platelets were labelled with a variety of lipophilic and thiol reagent spin probes. Aggregation was not affected by the lipid probes but was blocked by the covalent protein-directed probes. ESR spectra before and after thrombin treatment indicated that lipid structure of the platelet membrane was not affected to any measurable extent ...
F, Sachs, R D, Feinman
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Physics and chemistry of spin labels
Quarterly Reviews of Biophysics, 1970Biological systems provide the physical chemist with an abundance of interesting, challenging and significant problems. One example is the problem of the molecular basis of co-operative or allosteric interactions between distant ligand or substrate binding sites in hemoglobin and in enzymes.
H M, McConnell, B G, McFarland
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Biochemical and Biophysical Research Communications, 1972
Summary The facultative yeast cells have been spin-labeled biosynthetically with the 12-nitroxide stearic acid label under aerobic as well as anaerobic conditions. ESR spectra of the aerobic cells were different from those of the anaerobic cells. The signal intensity of the aerobic cells decreased upon removal of oxygen from the culture.
M, Nakamura, S I, Onishi
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Summary The facultative yeast cells have been spin-labeled biosynthetically with the 12-nitroxide stearic acid label under aerobic as well as anaerobic conditions. ESR spectra of the aerobic cells were different from those of the anaerobic cells. The signal intensity of the aerobic cells decreased upon removal of oxygen from the culture.
M, Nakamura, S I, Onishi
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Recent developments in spin labelling
Physics in Medicine and Biology, 1998Enhancements in spin-lattice relaxation rates of spin-labelled systems are considered both in terms of recent advances in nonlinear EPR methodology and in terms of the structural and dynamic biophysical information that can be obtained from such measurements at the molecular level.
Marsh, D., Livshits, V.
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Spin-labeling of immunoglobulin carbohydrates
Immunochemistry, 1978Abstract The oligosaccharide units of different immunoglobulins were spin-labeled after periodate oxidation. Electron spin resonance spectra of the spin-labeled immunoglobulins point out on the marked freedom of rotation of bound spin-label. Addition of antigen to the spin-labeled anti-human IgG antibodies does not result in any change of spectra.
R S, Nezlin +3 more
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