Results 301 to 310 of about 2,427,104 (377)
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Photosynthesis Research, 2009
Site-directed spin labeling in combination with electron paramagnetic resonance spectroscopy has emerged as an efficient tool to elucidate the structure and conformational dynamics of biomolecules under native-like conditions. This article summarizes the basics as well as recent progress of site-directed spin labeling.
Johann P, Klare +1 more
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Site-directed spin labeling in combination with electron paramagnetic resonance spectroscopy has emerged as an efficient tool to elucidate the structure and conformational dynamics of biomolecules under native-like conditions. This article summarizes the basics as well as recent progress of site-directed spin labeling.
Johann P, Klare +1 more
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Triplet-state spin labels for highly sensitive pulsed dipolar spectroscopy
Molecular Physics, 2018Pulsed dipolar spectroscopic methods allow nanometer distance measurements between pairs of spin labels. We have proposed a new spin labelling approach, based on the population of a chromophore triplet under light excitation, testing it on a peptide ...
M. G. D. Farra +4 more
semanticscholar +1 more source
Vision Research, 1975
Iodoacetamide and its spin-labeled derivative in high concentration ranges (10−2−10−1M) react with unexposed SH-groups of rhodopsin in the dark. The spin-labels bound to these SH-groups are moderately immobilized. While rhodopsin is not spin-labeled with the iodoacetamide at a lower concentration (5 × 10−4 m) in the dark, photobleached rhodopsin is ...
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Iodoacetamide and its spin-labeled derivative in high concentration ranges (10−2−10−1M) react with unexposed SH-groups of rhodopsin in the dark. The spin-labels bound to these SH-groups are moderately immobilized. While rhodopsin is not spin-labeled with the iodoacetamide at a lower concentration (5 × 10−4 m) in the dark, photobleached rhodopsin is ...
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Alkylating spin labels for spin-labelling of DNA
International Journal of Biochemistry, 1983Abstract 1. 1. Five alkylating spin labels, termed SL1, SL2, SL3, SL4 and SL5, have been synthesized and their suitability for spin-labelling of DNA was studied. 2. 2. It was found that one of them (SL5) lost its free radical moiety under the conditions of spin-labelling, thus giving DNA with no EPR signal. 3. 3.
I. Ivanov +4 more
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Spin-labelled Au nanoparticles
Faraday Discussions, 2004A series of Au nanoparticles functionalised with nitroxide spin labels has been prepared and studied by EPR spectroscopy. Samples with low coverage of the spin label were used to investigate the dynamics of the surface-attached labels at different distances from the Au surface. The rotational correlation times of spin labels vary from 10(-10) s to more
Victor, Chechik +6 more
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Thrombosis Research, 1976
Abstract Human platelets were labelled with a variety of lipophilic and thiol reagent spin probes. Aggregation was not affected by the lipid probes but was blocked by the covalent protein-directed probes. ESR spectra before and after thrombin treatment indicated that lipid structure of the platelet membrane was not affected to any measurable extent ...
F, Sachs, R D, Feinman
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Abstract Human platelets were labelled with a variety of lipophilic and thiol reagent spin probes. Aggregation was not affected by the lipid probes but was blocked by the covalent protein-directed probes. ESR spectra before and after thrombin treatment indicated that lipid structure of the platelet membrane was not affected to any measurable extent ...
F, Sachs, R D, Feinman
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Quarterly Reviews of Biophysics, 1981
The spin label method developed by McConnell 15 years ago is now widely used in studies of the structure and dynamic properties of a variety of the biological systems such as proteins and protein complexes, lipids and membranes, nucleic acids, nucleoproteins, etc.The ESR spectrum of the nitroxide radcal – the spin label – is very sensitive to its ...
S G, Kamzolova, G B, Postnikova
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The spin label method developed by McConnell 15 years ago is now widely used in studies of the structure and dynamic properties of a variety of the biological systems such as proteins and protein complexes, lipids and membranes, nucleic acids, nucleoproteins, etc.The ESR spectrum of the nitroxide radcal – the spin label – is very sensitive to its ...
S G, Kamzolova, G B, Postnikova
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Biochemical and Biophysical Research Communications, 1972
Summary The facultative yeast cells have been spin-labeled biosynthetically with the 12-nitroxide stearic acid label under aerobic as well as anaerobic conditions. ESR spectra of the aerobic cells were different from those of the anaerobic cells. The signal intensity of the aerobic cells decreased upon removal of oxygen from the culture.
M, Nakamura, S I, Onishi
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Summary The facultative yeast cells have been spin-labeled biosynthetically with the 12-nitroxide stearic acid label under aerobic as well as anaerobic conditions. ESR spectra of the aerobic cells were different from those of the anaerobic cells. The signal intensity of the aerobic cells decreased upon removal of oxygen from the culture.
M, Nakamura, S I, Onishi
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Electron Spin Resonance: Spin Labels
1981In general biological membranes possess no intrinsic paramagnetism and hence in the unlabeled state do not give rise to an electron spin resonance (ESR) spectrum. The introduction of a stable free radical (“spin label”) thus enables one to use ESR spectroscopy to study specific environments within the membrane.
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Biosynthesis of spin-labeled peptidoglycan: spin-spin interactions
Biochemistry, 1977Membrane preparations from Gaffkya homari catalyzed the in vitro biosynthesis of soluble uncross-linked spin-labeled peptidoglycan, a uniformly labeled polynitroxide, from the spin-labeled nucleotide UDP-MurNAc-Ala-DGlu-Lys(Nepsilon-2,2,5,5-tetramethyl-1-pyrrolin-1-oxyl-3-carbonyl)-DAla-DAla (I) and UDP-GlcNAc.
L S, Johnston, F C, Neuhaus
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