Results 31 to 40 of about 37,070 (307)

Spin Labeling of RNA Using “Click” Chemistry for Coarse-grained Structure Determination via Pulsed Electron-electron Double Resonance Spectroscopy

Bio-Protocol, 2021
Understanding the function of oligonucleotides on a molecular level requires methods for studying their structure, conformational changes, and internal dynamics.
Maria Vicino, Tobias Hett, Olav Schiemann   +2 more
doaj   +1 more source

Perturbation of membrane dynamics in nerve cells as an early event during bilirubin-induced apoptosis

Journal of Lipid Research, 2002
Increased levels of unconjugated bilirubin, the end product of heme catabolism, impair crucial aspects of nerve cell function. In previous studies, we demonstrated that bilirubin toxicity may be due to cell death by apoptosis.
Cecília M.P. Rodrigues, Susana Solá, Rui E. Castro, Pedro A. Laires, Dora Brites, José J.G. Moura   +5 more
doaj   +1 more source

An efficient EPR spin-labeling method enables insights into conformational changes in DNA

Biophysical Reports
Electron paramagnetic resonance (EPR) is a powerful tool for elucidating both static and dynamic conformational alterations in macromolecules. However, to effectively utilize EPR for such investigations, the presence of paramagnetic centers, known as ...
Melanie Hirsch, Lukas Hofmann, Idan Yakobov, Shirin Kahremany, Hila Sameach, Yulia Shenberger, Lada Gevorkyan-Airapetov, Sharon Ruthstein   +7 more
doaj   +1 more source

Detection of molecular transitions with nitrogen-vacancy centers and electron-spin labels

npj Quantum Information, 2022
We present a protocol that detects molecular conformational changes with two nitroxide electron-spin labels and a nitrogen-vacancy (NV) center in diamond.
C. Munuera-Javaloy, R. Puebla, B. D’Anjou, M. B. Plenio, J. Casanova   +4 more
doaj   +1 more source

A Computational Journey across Nitroxide Radicals: From Structure to Spectroscopic Properties and Beyond

Molecules, 2021
Nitroxide radicals are characterized by a long-lived open-shell electronic ground state and are strongly sensitive to the chemical environment, thus representing ideal spin probes and spin labels for paramagnetic biomolecules and materials.
Vincenzo Barone, Marco Fusè, Sandra Mónica Vieira Pinto, Nicola Tasinato   +3 more
doaj   +1 more source

Effects of a high-fat diet on superoxide anion generation and membrane fluidity in liver mitochondria in rats

Journal of the International Society of Sports Nutrition, 2018
Background Obesity is a primary factor of lifestyle-related diseases, and the age of its onset has decreased. The reactive oxygen species (ROS), the superoxide anion, is generated in the mitochondrial electron transport chain and the damage it induces in
M Togo, N Konari, M Tsukamoto, R Kimoto, T Yamaguchi, H Takeda, I Kambayashi   +6 more
doaj   +1 more source

Time after time – circadian clocks through the lens of oscillator theory

FEBS Letters, EarlyView.
Oscillator theory bridges physics and circadian biology. Damped oscillators require external drivers, while limit cycles emerge from delayed feedback and nonlinearities. Coupling enables tissue‐level coherence, and entrainment aligns internal clocks with environmental cues.
Marta del Olmo, Carolin Ector, Hanspeter Herzel   +2 more
wiley   +1 more source

Site Selective and Efficient Spin Labeling of Proteins with a Maleimide-Functionalized Trityl Radical for Pulsed Dipolar EPR Spectroscopy

Molecules, 2019
Pulsed dipolar electron paramagnetic resonance spectroscopy (PDS) in combination with site-directed spin labeling (SDSL) of proteins and oligonucleotides is a powerful tool in structural biology.
J. Jacques Jassoy, Caspar A. Heubach, Tobias Hett, Frédéric Bernhard, Florian R. Haege, Gregor Hagelueken, Olav Schiemann   +6 more
doaj   +1 more source

Probing Small-Angle Molecular Motions with EPR Spectroscopy: Dynamical Transition and Molecular Packing in Disordered Solids

Magnetochemistry, 2022
Disordered molecular solids present a rather broad class of substances of different origin—amorphous polymers, materials for photonics and optoelectronics, amorphous pharmaceutics, simple molecular glass formers, and others.
Sergei A. Dzuba
doaj   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source