Results 51 to 60 of about 2,427,104 (377)

Spin Labeling of RNA Using “Click” Chemistry for Coarse-grained Structure Determination via Pulsed Electron-electron Double Resonance Spectroscopy

Bio-Protocol, 2021
Understanding the function of oligonucleotides on a molecular level requires methods for studying their structure, conformational changes, and internal dynamics.
Maria Vicino, Tobias Hett, Olav Schiemann   +2 more
doaj   +1 more source

A Limited Symmetry Found by Comparing Calculated Magnetic Dipole Spin and Orbital Strengths in $^4\mbox{He}$

, 1994
Allowing for $2$$\hbar \omega$ admixtures in $^4\mbox{He}$ we find that the summed magnetic dipole isovector orbital and spin strengths are equal. This indicates a symmetry which is associated with interchanging the labels of the spin with those of the ...
A. Richter, B. A. Brown, B. Desplanques, D. C. Zheng, E. Lipparini, G. Orlandini, K. A. Snover, L. Zamick, L. Zamick, M. S. Fayache, W. Gross, W. Steffen   +11 more
core   +2 more sources

Site Selective and Efficient Spin Labeling of Proteins with a Maleimide-Functionalized Trityl Radical for Pulsed Dipolar EPR Spectroscopy

Molecules, 2019
Pulsed dipolar electron paramagnetic resonance spectroscopy (PDS) in combination with site-directed spin labeling (SDSL) of proteins and oligonucleotides is a powerful tool in structural biology.
J. Jacques Jassoy, Caspar A. Heubach, Tobias Hett, Frédéric Bernhard, Florian R. Haege, Gregor Hagelueken, Olav Schiemann   +6 more
doaj   +1 more source

An efficient EPR spin-labeling method enables insights into conformational changes in DNA

Biophysical Reports
Electron paramagnetic resonance (EPR) is a powerful tool for elucidating both static and dynamic conformational alterations in macromolecules. However, to effectively utilize EPR for such investigations, the presence of paramagnetic centers, known as ...
Melanie Hirsch, Lukas Hofmann, Idan Yakobov, Shirin Kahremany, Hila Sameach, Yulia Shenberger, Lada Gevorkyan-Airapetov, Sharon Ruthstein   +7 more
doaj   +1 more source

Effects of a high-fat diet on superoxide anion generation and membrane fluidity in liver mitochondria in rats

Journal of the International Society of Sports Nutrition, 2018
Background Obesity is a primary factor of lifestyle-related diseases, and the age of its onset has decreased. The reactive oxygen species (ROS), the superoxide anion, is generated in the mitochondrial electron transport chain and the damage it induces in
M Togo, N Konari, M Tsukamoto, R Kimoto, T Yamaguchi, H Takeda, I Kambayashi   +6 more
doaj   +1 more source

Detection of molecular transitions with nitrogen-vacancy centers and electron-spin labels

npj Quantum Information, 2022
We present a protocol that detects molecular conformational changes with two nitroxide electron-spin labels and a nitrogen-vacancy (NV) center in diamond.
C. Munuera-Javaloy, R. Puebla, B. D’Anjou, M. B. Plenio, J. Casanova   +4 more
doaj   +1 more source

A Computational Journey across Nitroxide Radicals: From Structure to Spectroscopic Properties and Beyond

Molecules, 2021
Nitroxide radicals are characterized by a long-lived open-shell electronic ground state and are strongly sensitive to the chemical environment, thus representing ideal spin probes and spin labels for paramagnetic biomolecules and materials.
Vincenzo Barone, Marco Fusè, Sandra Mónica Vieira Pinto, Nicola Tasinato   +3 more
doaj   +1 more source

Alamethicin self-assembling in lipid membranes: concentration dependence from pulsed EPR of spin labels [PDF]

, 2018
The antimicrobial action of the peptide antibiotic alamethicin (Alm) is commonly related to peptide self-assembling resulting in the formation of voltage-dependent channels in bacterial membranes, which induces ion permeation.
De Zotti, Marta, Dzuba, Sergei A, Formaggio, Fernando, Syryamina, Victoria N, Toniolo, Claudio   +4 more
core   +1 more source

A rigid disulfide-linked nitroxide side chain simplifies the quantitative analysis of PRE data [PDF]

, 2011
The measurement of 1H transverse paramagnetic relaxation enhancement (PRE) has been used in biomolecular systems to determine long-range distance restraints and to visualize sparsely-populated transient states.
Anthis, Nicholas J., Clore, Marius, Fawzi, Nicolas L., Fleissner, Mark R., Hideg, Kálmán, Hubbell, Wayne L., Kálai, Tamás   +6 more
core   +1 more source

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source