A fluorescence lifetime separation approach for FLIM live‐cell imaging
Abstract Fluorescence lifetime imaging microscopy (FLIM) translates the duration of excited states of fluorophores into lifetime information as an additional source of contrast in images of biological samples. This offers the possibility to separate fluorophores particularly beneficial in case of similar excitation spectra.
Cornelia Wetzker +8 more
wiley +1 more source
Lithium chloride at environmental concentrations impairs microtubule function and promotes genotoxicity in Allium cepa. [PDF]
Lima GG +10 more
europepmc +1 more source
Diploid origins and early genome stabilization in the allotetraploid Arabidopsis suecica
Summary Polyploidization, followed by genome downsizing, is a recurrent evolutionary cycle that dramatically reshapes genome structure. Newly formed polyploids must quickly adjust their cell division machinery to maintain stable chromosome inheritance, while long‐term stabilization involves rediploidization, returning the genome to a diploid‐like state.
Robin Burns +6 more
wiley +1 more source
Safeguarding genome integrity: Polo-like kinase Cdc5 and phosphatase Cdc14 orchestrate Topoisomerase II-mediated catenane resolution in mitosis. [PDF]
Massari LF +5 more
europepmc +1 more source
Epidemiological Insights Into Water and Soil‐Mediated Transmission of Tomato Mottle Mosaic Virus
Water and growing substrate can serve as reservoirs for tomato mottle mosaic virus (ToMMV) inoculum. ToMMV can survive in water for up to 27 weeks. ToMMV infects bait plants through roots within 5–17 weeks, depending on the severity of root damage. A rapid, field‐deployable RPA test—requiring no RNA extraction—offers sensitivity comparable to RT‐qPCR ...
Ana Vučurović +4 more
wiley +1 more source
Chromosomal Roadblocks in Male Fertility: Mechanisms, Risk Factors and Syndromes. [PDF]
Mitrakas AG +6 more
europepmc +1 more source
The multitalented TIP60 chromatin remodeling complex: wearing many hats in epigenetic regulation, cell division and diseases. [PDF]
Santopietro MV +4 more
europepmc +1 more source
Identification of locally activated spindle-associated proteins in oocytes uncovers a phosphatase-driven mechanism. [PDF]
Wan X +5 more
europepmc +1 more source
Comparative evaluation of dry, wet, and minimum quantity lubrication (MQL) cooling strategies in the machining of GTD-450 martensitic stainless steel. [PDF]
Dastgerdi HM +5 more
europepmc +1 more source

