Results 91 to 100 of about 1,368 (215)

Longitudinal Single‐Axon‐Resolution Imaging of Peripheral Nerve Injury Response in Mice Using an Optical Window Implant

Advanced Science, EarlyView.
A minimally invasive optical window enables stable, long‐term imaging of peripheral nerves in vivo at single‐axon resolution. Dynamic processes of degeneration, regeneration, and cellular remodeling are visualized across multiple time scales within the same nerve region, providing a unique platform to study a variety of anatomical and molecular events ...
Igor D. Luzhansky, Emma Anisman, Ron Perez, Sophia Zhang, Morgan Hoffman, Daniel Hunter, Abigail Cherian, Jevon Bonner, Emilia Feria, Ahmed Ahmed, Muneeb Malik, Junwei Du, Leland C. Sudlow, David M. Brogan, Matthew D. Wood, Mikhail Y. Berezin   +15 more
wiley   +1 more source

LRRC4 Orchestrates AP2A1‐Containing Clathrin‐Coated Vesicles to Disrupt Mitochondrial Cristae and Restrict Glioblastoma Progression

Advanced Science, EarlyView.
LRRC4 suppresses GBM by regulating AP2A1‐containing Golgi‐derived clathrin‐coated vesicles. Low LRRC4 allows cytoplasmic AP2A1 to maintain mitochondrial fission‐fusion, MICOS integrity, and OXPHOS, promoting proliferation and invasion. High LRRC4 redirects AP2A1‐containing Golgi‐derived clathrin‐coated vesicles to mitochondria, disrupts MICOS, enhances
Yang Li, Cheng Huang, Liangqi Jiang, Zhen Li, Qing Liu, Minghua Wu   +5 more
wiley   +1 more source

Biomimetic Gradient Hydrogels Regulate Osteochondral Regeneration Microenvironment Remodeling via Spatiotemporal Programming Engineering

Advanced Science, EarlyView.
Electric field‑driven continuous gradient hydrogel GHZF4 achieves spatiotemporal growth factor release, modulates immune microenvironment, angiogenesis and osteochondral differentiation. It realizes cell‑free functional osteochondral repair in rat/rabbit models, offering a promising biomimetic strategy for gradient tissue regeneration.
Xiaolian Niu, Shengzhao Xiao, Di Huang, Xuesong Wang, Yanwei Cao, Xiaodan Sun, Nicholas Dunne, Xiaoming Li   +7 more
wiley   +1 more source

scTIDE: Deciphering Critical Transitions Through Cell‐Perturbed Manifold Graphs and Optimal Transport Conditional Flow Matching

Advanced Science, EarlyView.
scTIDE identifies single‐cell tipping points by combining manifold‐based graph representations with optimal‐transport conditional flow matching, which preserves intrinsic topology and models distributional dynamics. It supports critical‐transition detection at individual‐cell resolution, prediction of unseen cells, and dimensionality reduction and ...
Jiayuan Zhong, Bowen Niu, Yongbo Yu, Shiyang Nie, Xuerong Gu, Pei Chen, Rui Liu   +6 more
wiley   +1 more source

Systemic Nanomechanical Single‐Cell Profiling Reveals Mechanophenotype Transitions Under Therapeutic Perturbation

Advanced Science, EarlyView.
Single‐cell mechanomics demonstrates that pharmacological perturbation changes cytoskeletal and cortical structure, suppressing cancer cell invasiveness. By integrating atomic force microscopy (AFM)‐based cortical measurements with stimulated emission depletion (STED)‐resolved adhesion and cytoskeletal organization, this approach identifies nanoscale ...
Minhee Ku, Jinwon Kwon, Nara Yoon, Hyung Kwon Byeon, Jaemoon Yang   +4 more
wiley   +1 more source

Ultrasound‐Activated Piezoelectric Neuroimmune Hydrogel Orchestrates Neurogenesis‐Macrophage Crosstalk in Diabetic Wound Healing

Advanced Science, EarlyView.
In this work, we design an ultrasound‐activated piezoelectric hydrogel platform (AOEF@AP5+US) that reprograms neurogenesis‐macrophage crosstalk as an integrated strategy to overcome the intertwined barriers of chronic inflammation, peripheral neuropathy, and impaired regeneration in diabetic wounds.
Kai Wang, Shaowen Zhuo, Binyu Song, Jinan Chen, Xin Zhao, Sijia Li, Shuang You, Dong Jiang, Yuedong Chen, Juanli Dang, Tong Wang, Baolin Guo, Zhou Yu, Baoqiang Song   +13 more
wiley   +1 more source

Human Foetal Neuroblasts Exhibit BK Channel‐Dependent Membrane Voltage Oscillations upon Depolarization

Advanced Science, EarlyView.
Primary cultures of neuroblasts isolated from the nucleus basalis of Meynert of 12‐weeks‐old human foetuses were prepared. Whole‐cell patch‐clamp recordings were performed by injecting a depolarizing stimulus current (+500 pA; 500 ms), and the membrane voltage recorded; this stimulus current evoked periodic‐like oscillations in membrane voltage ...
Elisabetta Coppi, Federica Cherchi, Martina Venturini, Federico Tommasi, Sandro Gonzi, Chiara Capacci, Giulia Guarnieri, Pasquale Gallina, Annamaria Morelli, Anna Maria Pugliese   +9 more
wiley   +1 more source

Genetic Ablation and Multi‐Omics Profiling Reveal CEP55 as a Key Driver of Tumorigenesis in Diverse Cancer Models

Advanced Science, EarlyView.
Genetic ablation of Cep55 in Pten‐deficient mouse models delays tumorigenesis. Integrated multi‐omics analyses (proteomics, phosphoproteomics, and spatial transcriptomics) reveal that CEP55 regulates oncogenic signaling (RAS/ERK, PI3K/AKT), integrin/FAK‐mediated adhesion, extracellular matrix (ECM) remodeling, and endocytosis.
Behnam Rashidieh, Yuanhao Yang, Amanda Louise Bain, Phillip Theron, Catharina Vosloo, Sahar Keshvari, Parinaz Ahangar, Brydie Bowden, Simon M. Tria, Ben B. Wang, Sandra Isenmann, Nicholas J. Westra van Holthe, Zherui Xiong, Hani Vu, Sowmya Sharma, Asmerom Sengal, J. Alejandro Lopez, John Finnie, Antonella Papa, Pascal H.G. Duijf, Quan Nguyen, Pirjo M. Apaja, Kum Kum Khanna   +22 more
wiley   +1 more source

COMPUTER SIMULATION OF VIBRATION SURVEILLANCE OF DISCRETELY IDEALISED MECHANICAL SYSTEMS. PART II: TOOL-WORKPIECE VIBRATION IN A NON-STATIONARY MODEL OF THE CUTTING PROCESS

TASK Quarterly, 2004
The problems of tool-workpiece vibration surveillance during machining have been researched. The object of investigation has been analysed, and cutting dynamics and calculation model creation described.
KRZYSZTOF J. KALIŃSKI
doaj  

In Situ Programmable Modulation of Hydrogel Stiffness for Stage‐Adaptive Bone Regeneration

Advanced Science, EarlyView.
Near‐infrared (NIR) irradiation triggers Ca2+ release from thermoresponsive nanocarriers, enabling in situ stiffening of an adaptive hydrogel from a soft to a stiff state, thereby sequentially supporting mesenchymal stromal cell stemness and osteogenic differentiation for stage‐adaptive bone regeneration.
Yuxin Yang, Fan Yang, Lu Wang, Zongtai Li, Weichang Li, Xinchun Zhang   +5 more
wiley   +1 more source