Results 121 to 130 of about 52,719 (293)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Help protect yourself from getting and spreading pink eye (conjuntivitis) [PDF]

open access: yes
Tips on how to help protect yourself from getting and spreading pink eye.CS260196-APublication date from document properties.protect-yourself ...

core  

The Origin and Evolution of Active Spreading Segments in the Northern Lau Basin

open access: yes
Extension at oceanic spreading centers ranges from ultra-slow (dominantly tectonic) to ultra-fast spreading (dominantly magmatic). This variation is reflected in the morphology of the spreading ridge segments and the magmatic productivity observed on the
Ryan, Michael
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

open access: yesFEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe   +5 more
wiley   +1 more source

Abrupt axial variations along the slow to ultra-slow spreading centers of the northern North Fiji Basin (SW Pacific): evidence for short wave heterogeneities in a back-arc mantle

open access: yes, 2003
This study presents results of surveys conducted along the slow to ultra-slow spreading axis of the Northern North Fiji Basin (NNFB), including the Hazel Holmes, Tripartite and South Pandora Ridges, and the newly discovered Futuna and North Cikobia ...
Pelletier, B.   +2 more
core   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

The Role of Libraries and Information Centers in Spreading Religious, Ethical, Social, Political, and Economic Awareness Reaching to Knowledge Society

open access: yes, 2007
This study aims at showing and reforming the role of libraries and information centers in men rooting and spreading out the religious and political doctrines as customs the tradition in side communities , and its role in facilitating dealing with recent ...
Ibrahem Nazmy Mohsen, Raed Soliman
core  

Home - About - Disclaimer - Privacy