Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
The effectiveness of progressive and traditional coaching strategies to improve iprint and jump performance across varying levels of maturation within a general youth population [PDF]
, 2019 Literature pertaining to youth development has identified the importance of understanding the physical, intellectual and emotional needs of adolescent youth.
Maulder, Peter S, Standing, Regan J
core +3 more sources
Optimal body size with respect to maximal speed for the yellow-spotted monitor lizard (Varanus panoptes; varanidae) [PDF]
, 2012 Studies of locomotor performance often link variation in morphology with ecology. While maximum sprint speed is a commonly used performance variable, the absolute limits for this performance trait are not completely understood. Absolute maximal speed has
Clemente, Christofer J. +2 more
core +2 more sources
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Applied SciencesBackground: Change of direction (COD) and sprint abilities are essential in soccer, while reactive agility involves decision-making and perceptual/cognitive skills.
Mohamed Amine Ltifi +3 more
doaj +1 more source
The effect of low volume sprint interval training in patients with non-alcoholic fatty liver disease [PDF]
, 2017 Objectives: Exercise is an important part of disease management in patients with non-alcoholic fatty liver disease (NAFLD), but adherence to current exercise recommendations is poor.
Babraj, John A. +3 more
core +5 more sources
The Relationship Between Acute: Chronic Workload Ratios and Injury Risk in Sports: A Systematic Review [PDF]
, 2020 Purpose: Low injury rates have previously been correlated with sporting team success, highlighting the importance of injury prevention programs. Recent methods, such as acute:chronic workload ratios (ACWR) have been developed in an attempt to predict and
Canetti, Elisa +3 more
core +1 more source
Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum
FEBS Open Bio, EarlyView.Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik +6 more
wiley +1 more source
Body size, skeletal maturity and functional characteristics of elite academy soccer players on entry between 1992 and 2003 [PDF]
, 2012 Secular changes in body size, estimated fatness, skeletal maturation, and functional characteristics of youth soccer players on entry into an elite academy between 1992 and 2003 were compared.
Carling, C., Le Gall, F.,, Malina, R.M.
core +1 more source
FEBS Open Bio, EarlyView.Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young +7 more
wiley +1 more source