Results 211 to 220 of about 53,752 (247)
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Crystallization and Preliminary X-Ray Crystallographic Studies on the Adenovirus ssDNA Binding Protein in Complex with ssDNA

Journal of Structural Biology, 1995
Crystals of the C-terminal domain of the adenovirus single-stranded DNA binding protein (DBP) in complex with the single-stranded oligonucleotide (dT)16 have been obtained by a batch method from material obtained by chymotryptic digest of full-length DBP. The colorless crystals grow as hexagonal prisms to a maximal size of approximately 0.85 x 0.55 x 0.
P N, Kanellopoulos   +4 more
openaire   +2 more sources

Selection and Application of ssDNA Aptamers against Clenbuterol Hydrochloride Based on ssDNA Library Immobilized SELEX

Journal of Agricultural and Food Chemistry, 2017
Clenbuterol hydrochloride (CLB) is often abused as additive feed for livestock to decrease adipose tissue deposition and to increase growth rate. It raises a potential risk to human health through the consumption of animal product. In this study, aptamers with higher affinity and specificity were screened through 16 selection rounds based on the ssDNA ...
Nuo Duan   +3 more
openaire   +2 more sources

Reaction of Cd(II)–Morin with dsDNA for biosensing of ssDNA oligomers with complementary, GCE-immobilized ssDNA

Bioelectrochemistry, 2008
In this work, the complex cadmium(II)-morin was synthesized and its interaction with double-stranded salmon sperm DNA was studied by electrochemical methods on glassy carbon electrode (GCE). It was shown that Cd(II)-Morin with high electrochemical activity can intercalate into the double-helix DNA, and the binding stoichiometry and equilibrium ...
Shuyan, Niu   +3 more
openaire   +2 more sources

Colorimetric detection of ssDNA in a solution

Current Applied Physics, 2009
Abstract A solution containing ssDNA was detected by using colloidal gold nanoparticles upon a color change of solution. First of all, we prepared colloidal gold nanoparticles with a size of less than 20 nm by means of citrate reduction of HAuCl 4 . Since colloidal gold nanoparticles modified with citrate anions have a negative charge, they are very ...
Sangchul Rho   +5 more
openaire   +1 more source

Counting ssDNA on a single nanoparticle

2008 IEEE Sensors, 2008
This paper describes the immobilization and quantification of 15base ssDNA on 250 nm silica nanoparticles. 1 up to 2400 amine functionalized ssDNA are coupled to a carboxyl functionalized nanoparticle using EDC/NHS chemistry. The amount of ssDNA on the nanoparticles is quantified by bulk fluorescence measurements in a microtiterplate reader on both the
F. Delport   +6 more
openaire   +1 more source

Functionalization, re-functionalization and rejuvenation of ssDNA nanotemplates

Chemical Communications, 2012
Single-stranded DNA (ssDNA) with repetitive sequence was demonstrated to be a versatile nanotemplate for introducing biological activity in a self-assembled manner. Re-functionalization and rejuvenation of the ssDNA nanotemplate were achieved under mild biological conditions without using high temperature and strong alkaline treatment to denature DNA.
Lin, Ming-Yu   +4 more
openaire   +3 more sources

The smallest ssDNA phage infecting a marine bacterium

Environmental Microbiology, 2018
Summary In the marine environment, only a few lytic single‐stranded DNA (ssDNA) phages have been isolated and characterized, despite the fact that diverse ssDNA bacteriophages have been discovered via metagenomic studies.
Yuanchao Zhan, Feng Chen
openaire   +3 more sources

ssDNA

2008
John B. Heppner   +104 more
openaire   +2 more sources

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