Results 191 to 200 of about 53,634 (224)
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Crystallization and Preliminary X-Ray Crystallographic Studies on the Adenovirus ssDNA Binding Protein in Complex with ssDNA

Journal of Structural Biology, 1995
Crystals of the C-terminal domain of the adenovirus single-stranded DNA binding protein (DBP) in complex with the single-stranded oligonucleotide (dT)16 have been obtained by a batch method from material obtained by chymotryptic digest of full-length DBP. The colorless crystals grow as hexagonal prisms to a maximal size of approximately 0.85 x 0.55 x 0.
P N, Kanellopoulos   +4 more
openaire   +2 more sources

Reaction of Cd(II)–Morin with dsDNA for biosensing of ssDNA oligomers with complementary, GCE-immobilized ssDNA

Bioelectrochemistry, 2008
In this work, the complex cadmium(II)-morin was synthesized and its interaction with double-stranded salmon sperm DNA was studied by electrochemical methods on glassy carbon electrode (GCE). It was shown that Cd(II)-Morin with high electrochemical activity can intercalate into the double-helix DNA, and the binding stoichiometry and equilibrium ...
Shuyan, Niu   +3 more
openaire   +2 more sources

Colorimetric detection of ssDNA in a solution

Current Applied Physics, 2009
Abstract A solution containing ssDNA was detected by using colloidal gold nanoparticles upon a color change of solution. First of all, we prepared colloidal gold nanoparticles with a size of less than 20 nm by means of citrate reduction of HAuCl 4 . Since colloidal gold nanoparticles modified with citrate anions have a negative charge, they are very ...
Sangchul Rho   +5 more
openaire   +1 more source

Counting ssDNA on a single nanoparticle

2008 IEEE Sensors, 2008
This paper describes the immobilization and quantification of 15base ssDNA on 250 nm silica nanoparticles. 1 up to 2400 amine functionalized ssDNA are coupled to a carboxyl functionalized nanoparticle using EDC/NHS chemistry. The amount of ssDNA on the nanoparticles is quantified by bulk fluorescence measurements in a microtiterplate reader on both the
F. Delport   +6 more
openaire   +1 more source

Functionalization, re-functionalization and rejuvenation of ssDNA nanotemplates

Chemical Communications, 2012
Single-stranded DNA (ssDNA) with repetitive sequence was demonstrated to be a versatile nanotemplate for introducing biological activity in a self-assembled manner. Re-functionalization and rejuvenation of the ssDNA nanotemplate were achieved under mild biological conditions without using high temperature and strong alkaline treatment to denature DNA.
Lin, Ming-Yu   +4 more
openaire   +3 more sources

The smallest ssDNA phage infecting a marine bacterium

Environmental Microbiology, 2018
Summary In the marine environment, only a few lytic single‐stranded DNA (ssDNA) phages have been isolated and characterized, despite the fact that diverse ssDNA bacteriophages have been discovered via metagenomic studies.
Yuanchao Zhan, Feng Chen
openaire   +3 more sources

Selection and identification of ssDNA aptamers recognizing zearalenone

Analytical and Bioanalytical Chemistry, 2013
Zearalenone (ZEN) is a nonsteroidal estrogenic mycotoxin produced by Fusarium graminearum on maize and barley. Because most current methods of ZEN detection rely on the use of low-stability antibodies or expensive equipment, we sought to develop a rapid, low-cost determination method using aptamers instead of antibodies as the specific recognition ...
Xiujuan, Chen   +8 more
openaire   +2 more sources

ssDNA

2008
John B. Heppner   +104 more
openaire   +2 more sources

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